A kind of bacterial cellulose/lactoferrin antibacterial dressing and preparation method thereof
A technology of bacterial cellulose and bacterial cellulose film, which is applied in bandages, absorbent pads, medical science, etc., can solve the problems of large protein solution volume, affecting BC performance, and unfavorable wound recovery, etc., and achieves a simple and easy preparation method , obvious advantages, short time-consuming effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0025] (1) Mix 50mL of 0.1M Tris solution with 42mL of 0.1M HCl solution, set the volume to 100mL (pH 7.4), then steam sterilize under the pressure of 0.08-0.1MPa for 20-30min, and obtain the protein buffer by refrigeration;
[0026] (2) Acetobacter xylinum was used as the strain, after static culture in liquid medium at constant temperature, placed in sodium hydroxide solution, treated at 80°C for 3 hours, taken out, rinsed with deionized water until neutral, the obtained bacteria Cellulose film; Soak in the protein buffer solution of step (1), obtain the bacterial cellulose film after the protein buffer solution soaks;
[0027] (3) Weigh 5 mg of lactoferrin and dissolve it in 10 mL of the protein buffer solution of step (1), fully dissolve it, filter it through a 0.22 μm filter membrane, and refrigerate to obtain a lactoferrin solution with a concentration of 0.5 mg / mL;
[0028] (4) the bacterial cellulose membrane soaked in the protein buffer solution in step (2) and the la...
Embodiment 2
[0031] The protein buffer prepared in step (1) of Example 1 and the bacterial cellulose membrane soaked in the protein buffer prepared in step (2) were used for reaction.
[0032] (1) Weigh 10 mg of lactoferrin and dissolve it in 10 mL of protein buffer solution. After fully dissolving, filter through a 0.22 μm filter membrane and refrigerate to obtain a lactoferrin solution with a concentration of 1 mg / mL;
[0033] (2) The bacterial cellulose membrane soaked in protein buffer solution and the lactoferrin solution in step (1) are placed in the electric field generating device, with the bacterial cellulose lower section ferritin solution as the negative electrode, and the upper section lactoferrin solution After being treated with 80V DC voltage, lactoferrin actively and directionally diffuses into the inside of bacterial cellulose from the cross section of bacterial cellulose, and the composite film is taken out from the electric field generator, rinsed with deionized water, an...
Embodiment 3
[0035] The protein buffer prepared in step (1) of Example 1 and the bacterial cellulose membrane soaked in the protein buffer prepared in step (2) were used for reaction.
[0036] (1) Weigh 50 mg of lactoferrin and dissolve it in 10 mL of protein buffer solution. After fully dissolving, filter through a 0.22 μm filter membrane and refrigerate to obtain a lactoferrin solution with a concentration of 5 mg / mL;
[0037](2) The bacterial cellulose membrane soaked in protein buffer solution and the lactoferrin solution in step (1) are placed in the electric field generating device, with the bacterial cellulose lower section ferritin solution as the negative electrode, and the upper section lactoferrin solution After being treated with 80V DC voltage, lactoferrin actively and directionally diffuses into the inside of bacterial cellulose from the cross section of bacterial cellulose, and the composite film is taken out from the electric field generator, rinsed with deionized water, and...
PUM
| Property | Measurement | Unit |
|---|---|---|
| concentration | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More