Unlock instant, AI-driven research and patent intelligence for your innovation.

Ferromagnetic nano carrier material/gene complex and preparation method and application thereof

A nanocarrier and ferromagnetic technology, which is applied in the field of carrier/gene complex preparation to achieve the effects of high reproducibility, strong popularization and low cost

Active Publication Date: 2017-12-26
ZHEJIANG UNIV
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of iron oxide nanocubes as gene transfection carriers at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ferromagnetic nano carrier material/gene complex and preparation method and application thereof
  • Ferromagnetic nano carrier material/gene complex and preparation method and application thereof
  • Ferromagnetic nano carrier material/gene complex and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1: the synthesis of ferric oxide nanocube (FION)

[0055] Accurately weigh 0.706g of iron acetylacetonate, 0.4g of biphenyl-4-carboxylic acid and 1.27g of oleic acid, then add 10.4g of high boiling point solvent dibenzyl ether, and mix evenly under stirring. The mixed solution was evacuated at room temperature for 1 hour, and then the temperature was raised to about 290°C at a rate of 20°C / min using a temperature controller, and then kept heating at this temperature for 0.5h, and then rapidly cooled to room temperature, and the product was washed with ethanol Resuspended in chloroform and stored, the resulting nanocubes had a size of 22-25 nm.

Embodiment 2

[0056] Embodiment 2: the synthesis of ferric oxide nanocube (FION)

[0057] Accurately weigh 0.706g of iron acetylacetonate, 0.4g of biphenyl-4-carboxylic acid and 1.27g of oleic acid, then add 10.4g of high boiling point solvent dibenzyl ether, and mix evenly under stirring. The mixed solution was evacuated at room temperature for 1 hour, then the temperature was raised to about 300°C at a rate of 20°C / min using a temperature controller, and then kept heating at this temperature for 1 hour, and then rapidly cooled to room temperature, and the product was washed with ethanol and reassembled. Suspended in chloroform for preservation, the resulting nanocube size is 30-35nm.

Embodiment 3

[0058] Embodiment 3: the synthesis of ferromagnetic nano-carrier material

[0059] 1) The iron ferric oxide nano-cube (FION), polycaprolactone (PCL) and oleic acid (OA) synthesized in Example 1 are added successively a mixed solvent of 4mL chloroform and tetrahydrofuran in a mass ratio of 10:2:1 (vol / vol=1), stirring at 400 rpm for 2 h under mechanical stirring.

[0060] 2) Weigh the excess PEI, after it is fully dissolved in the mixed solvent of 4mL chloroform and THF (vol / vol=1), add it dropwise to the mixed solution in the above 1) under stirring, and continue to stir at 400 rpm for 2h .

[0061] 3) Add 10 mL of double-distilled water to the mixed solution in the above 2), and ultrasonicate the probe for 5 minutes at a power of 500 W to obtain a uniform brown emulsion. Evaporate under reduced pressure on a rotary evaporator to remove the organic solvent, and obtain iron that is stably dispersed in the aqueous phase system. Magnetic Nanomaterials. After centrifuging the o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention relates to a ferromagnetic nano carrier material / gene complex including a ferromagnetic nano carrier material, and a target gene and a second cationic polymer which are successively adsorbed on the ferromagnetic nano carrier material through electrostatic attractive interaction; the ferromagnetic nano carrier material includes an inner core which is a ferroferric oxide nano cube, a middle layer modified by polycaprolactone and oleic acid, and an outer layer modified with a first cationic polymer by electrostatic attractive interaction; the middle layer consists of two layers of oleic acid and polycaprolactone connected with hydrophobic ends of the two layers of oleic acid. The invention also relates to a preparation method and an application of the ferromagnetic nano carrier material / gene complex. The ferromagnetic nano carrier material / gene complex can rely on self good ferromagnetism to achieve efficient gene transfection without depending on an external magnetic field.

Description

technical field [0001] The invention relates to the field of carrier / gene complex preparation, in particular to a ferromagnetic nano-carrier material / gene complex and its preparation method and application. Background technique [0002] Stem cell therapy is a new type of disease treatment that has emerged in recent years. Stem cells have the potential to differentiate into a variety of specific cells, and can secrete a variety of cytokines to participate in the repair of damaged tissues. Therefore, it has been widely used in the treatment of diseases such as tissue regeneration, diabetes, myocardial infarction and liver injury. Clinically, stem cell therapy has also been used for regenerative treatment of bone and cartilage damage, etc. [0003] However, stem cell therapy at this stage still faces many challenges, one of the important bottlenecks is how to efficiently and safely genetically recombine stem cells to further promote its therapeutic efficacy. [0004] In rece...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/87
CPCC12N15/87
Inventor 凌代舜张添源高建青
Owner ZHEJIANG UNIV