Superhigh-temperature aerobic fermentation microbial agent prepared from municipal sludge and preparation method of superhigh-temperature aerobic fermentation microbial agent

A technology for municipal sludge and aerobic fermentation, applied in microorganism-based methods, biochemical equipment and methods, sewage/sludge fertilizers, etc. and other problems, to achieve the effect of improving the survival rate and preservation period, protecting and improving the ecological environment, and solving the difficulties of fermentation production

Pending Publication Date: 2018-01-19
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, extreme thermophilic bacteria have harsh growth conditions and are difficult to preserve. Moreover, the nutrient content in municipal sludge is limited and the composition is unstable. If sludge is directly used as the only raw materia

Method used

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  • Superhigh-temperature aerobic fermentation microbial agent prepared from municipal sludge and preparation method of superhigh-temperature aerobic fermentation microbial agent
  • Superhigh-temperature aerobic fermentation microbial agent prepared from municipal sludge and preparation method of superhigh-temperature aerobic fermentation microbial agent
  • Superhigh-temperature aerobic fermentation microbial agent prepared from municipal sludge and preparation method of superhigh-temperature aerobic fermentation microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example 1 Production of Calditerricolasatsumensis super-temperature aerobic fermentation inoculum

[0023] (1) Municipal sludge samples

[0024] Take the concentrated sludge from a sewage treatment plant in Beijing. The basic properties of the sludge are shown in Table 1.

[0025] Table 1. Basic properties of tested sludge

[0026]

[0027] (2) Configuration of sludge fermentation medium

[0028] Add water to adjust the solid content of the sludge to 2%, add 0.2% foam enemy, 1g / L yeast powder, 0.5g / L ammonium chloride, 0.3g / L sucrose, 0.2g / L soluble starch, 0.3g / L phosphoric acid Potassium dihydrogen, adjust the pH to 7.0, add 50g / L biochar / wheat bran (w / w=2 / 1) composite carrier, stir well, sterilize at 100°C for 45 minutes, and cool to 70-80°C for use.

[0029] (3) Shake flask culture Pick colonies from the normally preserved Calditerricolasatsumensis FAFU012 plate medium, inoculate them into a 300 mL Erlenmeyer flask containing 100 mL CYS liquid medium, and culture for 12 hours...

Example Embodiment

[0038] Example 2 Production of Calditerricolayamamurae ultra-high temperature aerobic fermentation inoculum

[0039] (1) Municipal sludge samples

[0040] Take the concentrated sludge from Zhengzhou City, Henan Province. The basic properties of the sludge are shown in Table 2.

[0041] Table 2 Basic properties of test sludge

[0042]

[0043] (2) Configuration of sludge fermentation medium

[0044] Add water to adjust the solid content of the sludge to 3%, add 0.2% foam enemy, 1.5g / L yeast powder, 1.0g / L ammonium chloride, 0.5g / L sucrose, 0.5g / L soluble starch, 0.5g / L Potassium dihydrogen phosphate, adjust the pH to 7.0, add 80g / L biochar / wheat bran (w / w=1.5 / 1) composite carrier, stir well, sterilize at 100°C for 45 minutes, and cool to 70-80°C for later use.

[0045] (3) Shake flask culture Pick colonies from the normally preserved Calditerricolayamamurae UTM801 plate medium, inoculate them into a 300 mL Erlenmeyer flask containing 100 mL of CYS liquid medium, and culture for 12 hours ...

Example Embodiment

[0054] Example 3 Production of Thermusthermophilus ultra-high temperature aerobic fermentation inoculum

[0055] (1) Municipal sludge samples

[0056] Take the concentrated sludge from a sewage treatment plant in Fuzhou City, Fujian Province. The basic properties of the sludge are shown in Table 3.

[0057] Table 3. Basic properties of tested sludge

[0058]

[0059] (2) Configuration of sludge fermentation medium

[0060] Add water to adjust the solid content of the sludge to 3%, add 0.2% foam enemy, 1.5g / L yeast powder, 0.5g / L ammonium chloride, 0.5g / L sucrose, 0.5g / L soluble starch, 1g / L phosphoric acid Potassium dihydrogen, adjust the pH to 7.0, add 100g / L biochar / wheat bran (1.5 / 1) composite carrier, stir evenly, sterilize at 100°C for 45 minutes, and cool to 70-80°C for later use.

[0061] (3) Shake flask culture Pick colonies from the normal preserved Thermusthermophilus HB8 plate medium, inoculate them into a 300 mL Erlenmeyer flask containing 100 mL of CYS liquid medium, and cu...

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Abstract

The invention discloses a preparation method of a superhigh-temperature aerobic fermentation microbial agent prepared from municipal sludge. The preparation method comprises steps as follows: superhigh-temperature aerobic bacteria are activated and then fermented, a supernatant is removed from fermentation broth, a protective agent is added and is stirred and mixed uniformly, the mixture is driedand ground by a pulverizer, and a product is screened and subpackaged. The scheme has the advantages as follows: firstly, the low-cost municipal sludge is taken as a main fermentation raw material forregulation and preparation of a fermentation medium to realize fermentation production of the microbial agent, one idea is provided for solving the problem of pollution caused by the municipal sludge, protection and improvement of the ecological environment are facilitated, fermentation cost of the superhigh-temperature aerobic bacteria is reduced, and fermentation efficiency is improved; secondly, compared with a fermentation microbial agent prepared with a conventional method, survival rate and storage period of the superhigh-temperature aerobic fermentation microbial agent can be effectively increased with addition of a composite support and the protective agent, and the microbial agent is suitable for long-term storage; finally, the application cost of the superhigh-temperature aerobic bacteria is reduced, and the microbial agent and the preparation method are suitable for large-scale industrial production and application.

Description

technical field [0001] The invention relates to the field of microorganism cultivation and application, in particular to a preparation method of an ultra-high temperature aerobic fermentation bacterial agent prepared by utilizing municipal sludge and a preparation method thereof. Background technique [0002] High-temperature composting is an important means of harmless treatment and resource utilization of organic solid waste. Its essence is the process of catabolism of organic matter under the action of microorganisms to generate heat energy, which promotes the transformation of organic matter into stable humus. High temperatures are a double-edged sword for aerobic composting. On the one hand, the rapid decomposing and hygienic harmlessness of materials are inseparable from high temperature, which is conducive to the formation of humus and the killing of pathogenic bacteria; on the other hand, important components of microbial organisms such as proteins and nucleic acids ...

Claims

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Application Information

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IPC IPC(8): C12N1/20
CPCC12N1/04C12N1/20C05F7/00C12N1/205C12R2001/01C12N2500/10C12N2500/34
Inventor 周顺桂陈志廖汉鹏
Owner FUJIAN AGRI & FORESTRY UNIV
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