Biotransformation method for L-glufosinate

A technology of biotransformation and glufosinate-ammonium, applied in the field of biotransformation of L-glufosinate-ammonium, can solve the problems of harsh processing and manufacturing process, difficult to degrade D-type glufosinate-ammonium, difficult to meet production requirements, etc., and achieve catalytic effect Good, the effect of shortening the conversion time and increasing the yield

Inactive Publication Date: 2018-01-26
武汉茵茂特生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The synthesis process is at 500-600°C. If the control is unstable, it is easy to produce yellow phosphorus and phosphine, which are very easy to be natural, which is very dangerous.
In addition, the material is highly corrosive, and there are strict requirements on the material selection and manufacturing process of the reaction equipment. The current domestic processing and manufacturing level is diff...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation process of L-glufosinate-ammonium crystal comprises the steps:

[0035] The biotransformation method of L-glufosinate-ammonium, the reaction is carried out in a 1L shake flask, and the reaction system is controlled as 300mL, with 2-carbonyl-4-(hydroxymethylphosphono)butanoic acid of 30g as substrate, and MOPS buffer solution (with 3-morpholinopropanesulfonic acid and Na 2 PO 4 The normal saline solution that is the buffer pair) is solvent, with the glufosinate-ammonium dehydrogenase that derives from Saccharomyces cerevisiae of 22g / L, the whole cell of the genetically engineered bacteria that derives from the co-expression of formate dehydrogenase of Candida boidinii is catalyst, add 60g ammonium formate, an appropriate amount of coenzyme NADP+ and 0.165g additive for conversion reaction, adding to obtain a conversion solution containing L-glufosinate-ammonium. Wherein, the additive is a mixture of 0.1238g of apple extract powder and 0.0412g of spiruli...

Embodiment 2

[0038] The preparation process of L-glufosinate-ammonium crystal comprises the steps:

[0039] The biotransformation method of L-glufosinate-ammonium, the reaction is carried out in a 1L shake flask, and the reaction system is controlled as 300mL, with 30g of 2-carbonyl-4-(hydroxymethylphosphono)butanoic acid as substrate, and carbonate The buffer solution is used as a solvent, with 25g / L of glufosinate-ammonium dehydrogenase derived from Saccharomyces cerevisiae and the whole cell of genetically engineered bacteria co-expressed with formate dehydrogenase derived from Candida boidinii as a catalyst, 60g of ammonium formate is added, and an appropriate amount of coenzyme NADP+ and 0.375g of additives were converted into a conversion solution containing L-glufosinate-ammonium. Wherein, the additive is a mixture of 0.3g of apple extract powder and 0.075g of spirulina powder. The preparation method of the apple extract powder is as follows: crushing fresh apples, squeezing the ju...

Embodiment 3

[0042] The preparation process of L-glufosinate-ammonium crystal comprises the steps:

[0043] The biotransformation method of L-glufosinate-ammonium, the reaction is carried out in a 1L shake flask, and the reaction system is controlled as 300mL, with 30g of 2-carbonyl-4-(hydroxymethylphosphono)butanoic acid as substrate, buffered with phosphate The solution is used as a solvent, and 20 g / L of glufosinate-ammonium dehydrogenase derived from Saccharomyces cerevisiae and whole cells of genetically engineered bacteria co-expressed with formate dehydrogenase derived from Candida boidinii are used as catalysts, and 60 g of ammonium formate is added, and an appropriate amount of coenzyme NADP+ Perform transformation reaction with 0.18g additive to obtain transformation liquid containing L-glufosinate-ammonium. Wherein, the additive is a mixture of 0.15g of apple extract powder and 0.03g of spirulina powder. The preparation method of the apple extract powder is as follows: crush fr...

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PUM

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Abstract

The invention discloses a biotransformation method for L-glufosinate. The biotransformation method comprises the following steps: composing a reaction system from a solvent and 2-carbonyl-4-(hydroxymethylphosphoryl)butyric acid used as a substrate, and then adding a biocatalyst, coenzyme, an additive and an ammonium salt into the reaction system for a biotransformation reaction so as to obtain a transformed solution containing L-glufosinate, wherein the biocatalyst is the whole cell of a genetically engineered bacterium co-expressed by glufosinate dehydrogenase derived from Saccharomyces cerevisia and formate dehydrogenase derived from Candida boidinii; the coenzyme is NADP+; and the ammonium salt is ammonium formate. The biotransformation method is simple in process flow, free of specialrequirements on equipment and suitable for industrial production. HPLC-MS and HPLC are employed for monitoring until the substrate is fully utilized.

Description

technical field [0001] The invention relates to a preparation method of pesticides, in particular to a biotransformation method of L-glufosinate-ammonium. Background technique [0002] Glufosinate-ammonium, the chemical name is 4-[hydroxy(methyl)phosphono]-DL-homoalanine, which was developed and produced by Hearst (now Bayer, Germany) in the 1980s, and belongs to phosphonic acid Herbicides are glutamine synthesis inhibitors, non-selective contact herbicides, which were registered for use as herbicides in 1984. In 2004, my country only had the registration of glufosinate-ammonium technical, and in 2005, my country had product registration. [0003] Since glyphosate was widely used, glyphosate-resistant weeds have been increasing, and the harm has gradually increased. Paraquat is a strong herbicide that kills weeds and is highly toxic to humans and animals. On July 1, 2014, my country revoked the registration and production license of paraquat water solution, and stopped pr...

Claims

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Application Information

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IPC IPC(8): C12P13/04
Inventor 胡磊徐灿陈迈胡鹏高
Owner 武汉茵茂特生物技术有限公司
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