A method for determining total ginsenosides by gravimetric method

A technology of total ginsenosides, gravimetric method, applied in the direction of removing certain components and weighing, etc., can solve the problems of inability to guarantee the stability of the measurement results, the influence of the accuracy of the results, and the incomplete extraction of saponins, so as to maintain physical health. , easy to operate, simple process effect

Active Publication Date: 2020-08-11
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, through the research on the CAC method, it is found that the method has defects such as poor repeatability and incomplete extraction of saponins: 1. The efficiency of extracting saponins with water-saturated n-butanol is low; The precision of the results is poor; 3. Malonyl ginsenosides are very easy to transform, and the distribution coefficient in the water layer during the extraction process is high, so the stability of the measurement results cannot be guaranteed; 4. Ether is poured out directly after degreasing, which is extremely difficult to operate , which has a great influence on the accuracy of the results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The determination method of total ginsenosides in ginseng rhizomes is as follows:

[0045] 1) Dry the ginseng rhizome first, crush it, pass through a 60-mesh sieve, weigh 5g of the sample, accurate to 1mg, wrap it with filter paper, place the filter paper tube in a Soxhlet extractor, add 100ml of a mixed aqueous solution of methanol-ammonia ( Ammonia water volume percentage content is 3%, methanol volume percentage content is 70%), reflux extraction at 90°C for 8h;

[0046] 2) Purification: Collect the extraction solution into an evaporating dish, evaporate to dryness in a water bath, add 50 mL of water to wash the residue several times, combine the washing liquid into a separatory funnel, extract once with 50 mL of dichloromethane, let stand until the interface is clear, discard Remove the dichloromethane in the lower layer, collect the water layer into an evaporating dish, evaporate to dryness in a water bath, add 15 mL of water to wash the residue several times, comb...

Embodiment 2

[0053] The determination method of total ginsenosides in ginseng rhizomes is as follows:

[0054] 1) Dry the rhizome of ginseng at 45°C, crush it, pass it through a 70-mesh sieve, and weigh 2.5 g of the sample, accurate to 1 mg;

[0055] 2) Preparation of solid-phase extraction column: the solid-phase extraction column is a 2×20cm plastic column or glass column, add a layer of sieve plate at the bottom of the column, pour 8-12mL NM200 reverse-phase polymer, and add a layer of sieve plate on the upper layer plate, rinse the solid phase extraction column with 50-100mL methanol, replace the methanol with 200-300mL water before use, then install a syringe needle on the solid phase extraction column, and wait for the sample to be loaded;

[0056] 3) Purification: Dissolve the ginseng paste in 50mL water several times, combine the solution into a separatory funnel, extract once with 50mL dichloromethane, let stand until the interface is clear, discard the lower dichloromethane, coll...

Embodiment 3

[0063] The determination method of total ginsenosides in ginseng rhizomes is as follows:

[0064] 1) Dry the ginseng rhizome at 55°C, crush it, pass it through a 100-mesh sieve, and weigh 1.5 g of the sample, accurate to 1 mg;

[0065] 2) Preparation of solid-phase extraction column: the solid-phase extraction column is a 2×20cm plastic column or glass column, add a layer of sieve plate at the bottom of the column, pour 8-12mL NM200 reverse-phase polymer, and add a layer of sieve plate on the upper layer plate, rinse the solid phase extraction column with 50-100mL methanol, replace the methanol with 200-300mL water before use, then install a syringe needle on the solid phase extraction column, and wait for the sample to be loaded;

[0066] 3) Purification: After weighing the sample, add 50 mL of water to dissolve the sample several times and combine them in a separatory funnel, extract once with 50 mL of dichloromethane, let stand until the interface is clear, discard the lowe...

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Abstract

The invention provides a method for determining total ginsenosides by gravimetric method, which mainly includes the following steps: (A) drying and pulverizing the ginseng rhizome, and using a mixed aqueous solution of methanol-ammonia water to process and reflux extraction to obtain an extract; (B) Purify the residue after evaporating the solvent and drying the extract, and calculate the mass percentage content of the total saponins. The method for determining total ginsenosides in the embodiment of the present invention has the advantages of simple operation and good repeatability, which can not only ensure the consistency of the measurement results, but also meet the needs of small and medium-sized enterprises lacking equipment purchase funds to carry out ginsenoside detection work, avoiding the use of toxic Larger organic reagents are safe and environmentally friendly, and are more conducive to maintaining the health of operators. The operation time is short and the process is simple, which fills the technical gap of related determination methods, forms a relatively standard operation process, and requires low cost of supporting equipment , which is suitable for wide application and is worth learning from.

Description

technical field [0001] The invention relates to the field of extraction and determination of total ginsenosides in ginseng rhizomes, in particular to a method for determining total ginsenosides using a gravimetric method. Background technique [0002] Ginseng (scientific name: Panax ginseng, also known as Asian ginseng, native name "stick" in Northeast China) is a fleshy root that can be used medicinally. Ginseng belongs to the Araliaceae family and grows mainly in East Asia, especially in cold regions. Ginseng is a common medicinal material in Asia, and American ginseng is also commonly used in North and Central America. Many herbal shops and supermarkets can find a variety of ginseng decoction pieces and extract health products, which are used for recovery after recovery, enhance physical strength, regulate hormones, lower blood sugar and control blood pressure , control liver index and liver function health care, etc. The saponin contained in the root of ginseng is its ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N5/04
CPCG01N5/04
Inventor 逄世峰王英平王佳肖盛元
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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