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Myohemoglobin monoclonal antibody-enzyme composite compound and myohemoglobin content detection kit containing same

A monoclonal antibody, myoglobin technology, applied in the field of myoglobin measurement, can solve the problems of high environmental requirements, poor sensitivity, high price, etc., and achieve the effects of high calibration sensitivity, low resolution sensitivity, and improved sensitivity

Inactive Publication Date: 2018-03-09
ANHUI DAQIAN BIO ENG LIMITED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of the colloidal gold method is that it cannot be accurately quantified and the difference between batches is large, and it cannot continuously monitor the level of myoglobin in patients; the immunofluorescence method has the disadvantages of poor response time, high environmental requirements, and is easily affected by air dust 96 servings / box, the use of rare earth markers and other shortcomings also make this immune method unable to be used on a large scale; while most of the chemiluminescence immunoassays are imported reagents, which are expensive and are not conducive to popularization in primary hospitals
[0005] Immunoturbidimetry has become the preferred method for clinical determination because of its wide detection linear range, simple operation, easy operation, and high degree of automation. Therefore, this area needs a kind of myoglobin detection kit that can detect convenience, high sensitivity, be easy to popularize etc.

Method used

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  • Myohemoglobin monoclonal antibody-enzyme composite compound and myohemoglobin content detection kit containing same
  • Myohemoglobin monoclonal antibody-enzyme composite compound and myohemoglobin content detection kit containing same
  • Myohemoglobin monoclonal antibody-enzyme composite compound and myohemoglobin content detection kit containing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A kit for detecting myoglobin content by latex-enhanced immunoturbidimetric method, comprising R1 reagent, R2 reagent and myoglobin calibrator;

[0035] The R1 reagent consists of the following components:

[0036]

[0037] The R2 reagent consists of the following components:

[0038]

[0039] Described myoglobin calibrator is made up of the myoglobin solution that concentration is respectively 0ug / L, 100ug / L, 200ug / L, 400ug / L, 800ug / L, and solvent is the Tris-HCL solution of 100mM, and Tris-HCL The solution contains 0.5wt% bovine serum albumin, 0.3wt% PC-300 and 30mM disodium edetate;

[0040] The myoglobin monoclonal antibody-enzyme binding complex is prepared according to the following method steps:

[0041] a) cholinesterase is dissolved in a glutaraldehyde solution with a concentration of 5wt% by the addition of 10 mg / ml, left to stand at room temperature for 8 hours, and after dialysis with normal saline, add myoglobin monoclonal antibody, choline The mas...

Embodiment 2

[0044] A kit for detecting myoglobin content by latex-enhanced immunoturbidimetric method, comprising R1 reagent, R2 reagent and myoglobin calibrator;

[0045] The R1 reagent consists of the following components:

[0046]

[0047] The R2 reagent consists of the following components:

[0048]

[0049] Described myoglobin calibrator is made up of the myoglobin solution that concentration is respectively 0ug / L, 100ug / L, 200ug / L, 400ug / L, 800ug / L, and solvent is the Tris-HCL solution of 0mM, and Tris-HCL The solution contains 0.1wt% bovine serum albumin, 0.05% PC-300 and 5mM disodium edetate;

[0050] The myoglobin monoclonal antibody-enzyme binding complex is prepared according to the following method steps:

[0051] a) cholinesterase was dissolved in a glutaraldehyde solution with a concentration of 10wt% in an amount of 2 mg / ml, left to stand at room temperature for 8 hours, and after dialysis with normal saline, myoglobin monoclonal antibody and choline were added The...

Embodiment 3

[0054] A kit for detecting myoglobin content by latex-enhanced immunoturbidimetric method, comprising R1 reagent, R2 reagent and myoglobin calibrator;

[0055] The R1 reagent consists of the following components:

[0056]

[0057] The R2 reagent consists of the following components:

[0058]

[0059]

[0060] Described myoglobin calibrator is made up of the myoglobin solution that concentration is respectively 0ug / L, 100ug / L, 200ug / L, 400ug / L, 800ug / L, and solvent is the Tris-HCL solution of 200mM, and Tris-HCL The solution contains 1 wt% bovine serum albumin, 0.5 wt% PC-300 and 50 mM disodium edetate;

[0061] The myoglobin monoclonal antibody-enzyme binding complex is prepared according to the following method steps:

[0062] a) cholinesterase is dissolved in a glutaraldehyde solution with a concentration of 2wt% by the addition of 20 mg / ml, left to stand at room temperature for 8 hours, and after dialysis with normal saline, add myoglobin monoclonal antibody, cho...

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Abstract

The invention discloses a myohemoglobin monoclonal antibody-enzyme composite compound. The myohemoglobin monoclonal antibody-enzyme composite compound is obtained by cross-linking reaction between myohemoglobin monoclonal antibodies and cholinesterase. The invention further discloses a kit for detecting myohemoglobin content through latex enhanced immunoturbidimetry, and comprises a reagent R1 anda reagent R2. The reagent R2 comprises the myohemoglobin monoclonal antibody-enzyme composite compound. The problems of low sensitivity of common immunoturbidimetry and high price of chemiluminiscence immunoassay are solved, and the myohemoglobin monoclonal antibody-enzyme composite compound and the kit can be popularized widely.

Description

technical field [0001] The invention relates to the technical field of myoglobin measurement, in particular to a myoglobin monoclonal antibody-enzyme binding complex and a kit containing it for detecting myoglobin content. Background technique [0002] Myoglobin (MYO) is an oxygen-binding heme protein that stores oxygen in muscle, and its oxygen saturation curve is hyperbolic. It is mainly distributed in cardiac muscle and skeletal muscle tissue, accounting for about 0.1-0.2% of the total muscle mass. [0003] Determination of serum myoglobin can be used as an important indicator for early diagnosis of acute myocardial infarction (AMI). If Myo is negative, myocardial infarction is basically ruled out. Re-infarction or infarct extension. In addition Myo increased, also seen in the early stage of acute myocardial infarction, acute muscle injury, muscular dystrophy, muscular atrophy, polymyositis, acute or chronic renal failure, severe congestive heart failure and long-term s...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577
CPCG01N33/68G01N33/577G01N2333/805
Inventor 高帆
Owner ANHUI DAQIAN BIO ENG LIMITED
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