Biological softening method of areca seeds

A betel nut and biological technology, which is applied in the field of betel nut softening and biological softening of betel nut, can solve the problems of high production cost of betel nut, unsatisfactory softening effect, and slag of end products, and achieve controllable cellulose hydrolysis, prolonged time consumption, and hydrolysis controllable effect

Active Publication Date: 2018-03-16
长沙市一诺生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the use of different enzymes or compound enzyme preparations to soften betel nuts has disa

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kind of biological softening method of betel nut of the present invention, comprises the following steps:

[0030] (1) Preparation of fermentation broth containing microorganisms

[0031] Take 2 rings from the slope of the activated S. coronoidis strain and insert them into 50mL liquid medium, put them into a shaker incubator for cultivation, the rotation speed is 150r / min, the cultivation temperature is 25°C, and the cultivation time is 40h. Obtain a culture fluid, expand and cultivate step by step to the required fermentation broth amount by the inoculum size of 1% (v / v), obtain the fermentation fluid containing microorganism, the final concentration of microorganism in the fermentation fluid is 1.0 * 10 8 cfu / mL~9.0×10 8 cfu / mL.

[0032] The above liquid medium is mainly composed of sucrose, wort, yeast extract and water, the raw material concentration is: 20g / L sucrose, 10g / L wort, 2g / L yeast extract, natural pH, sterilized at 121°C for 15min.

[0033] (2) Biol...

Embodiment 2

[0037] A kind of biological softening method of betel nut of the present invention, comprises the following steps:

[0038] (1) Preparation of fermentation broth containing microorganisms

[0039] Take 2 rings from the slope of the activated S. coronoides strain and put them into 100mL liquid culture medium, carry out shaker culture, the rotation speed is 120r / min, the culture temperature is 22°C, and the culture time is 50h to obtain a primary culture solution According to the inoculum size of 2% (v / v), expand and cultivate step by step to the required amount of fermented liquid, obtain the fermented liquid containing microorganisms, and the final concentration of microorganisms in the fermented liquid is 1.0 × 10 9 cfu / mL~9.0×10 9 cfu / mL.

[0040] The above liquid medium is mainly composed of sucrose, wort, yeast extract and water, the raw material concentrations are as follows: 50g / L sucrose, 12g / L wort, 2g / L yeast extract, natural pH, sterilized at 121°C for 15min.

[004...

Embodiment 3

[0045] A kind of biological softening method of betel nut of the present invention, comprises the following steps:

[0046] (1) Preparation of fermentation broth containing microorganisms

[0047] per 1cm 2 Add 1mL of peptone solution with a mass fraction of 0.3% to the inclined surface of the test tube to elute and collect the strains on the inclined surface of the test tube of the activated S. 2 Add the eluate of the test tube strain on the surface of the solid medium, cultivate at 24°C, and cultivate for 65 hours. According to the amount of fermentation broth containing microorganisms required for subsequent production, expand the culture: according to the amount per 1cm 2 Add 0.4mL of peptone solution with a mass fraction of 0.3% to elute and collect the bacteria on the surface of the solid medium cultured last time to obtain the bacteria eluate, and then press 0.002mL / cm 2 Add the collected bacterial eluate on the surface of the newly prepared solid medium, cultivate at...

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Abstract

The invention discloses a biological softening method of areca seeds. The biological softening method comprises the following steps of deeply processing the areca seeds, placing the areca seeds afterfermentation and cleaning in fermentation liquid containing microorganisms, performing stirring and fermentation, and softening the areca seeds with complex enzymes generated by the microorganisms, sothat softened areca seeds are obtained, wherein the microorganisms are eurotium cristatum bacterial strains, the mass ratio of the areca seeds after fermentation and cleaning to the fermentation liquid containing the microorganisms is (1.0-25) to 100, the stirring speed is 10rpm-80rpm, the fermentation temperature is 25-40 DEG C, and the fermentation time is 12-48h. The biological softening method has the advantages of being high in softening effect, high in safety, capable of improving the biological activity of polyphenols of the areca seeds, high in eating quality and the like.

Description

technical field [0001] The invention belongs to the field of agricultural product processing, and relates to a method for softening areca nuts, in particular to a biological softening method for areca nuts. Background technique [0002] Betel nut is known as the top of southern medicine. It has a history of thousands of years of cultivation in my country, and it is also used as a special product for local residents to eat fresh. Edible betel nut dried fruit originated in Hunan and has a history of nearly 300 years. In 2016, the annual output value of edible dried betel nut processing industry reached 20 billion yuan, and it is developing rapidly at an annual growth rate of 15-20%. Due to the low water content and high hardness of the dried betel nut product, its fibers will cause a certain degree of damage to the oral mucosa and teeth during chewing, which may lead to oral mucosal fibrosis and oral cancer. In order to improve the quality of edible dried betel nuts and impro...

Claims

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Application Information

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IPC IPC(8): A23L19/00A23L29/00C12N1/14C12R1/645
CPCA23L19/00A23L29/065C12N1/14
Inventor 李宗军李珂
Owner 长沙市一诺生物科技有限责任公司
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