The invention belongs to the field of polymer materials, and particularly relates to an ultra-low temperature-resistant toughening material for injection molding-grade nylon and a preparation method of the toughening material. Raw materials of the ultra-low temperature-resistant toughening material comprise: in parts by weight, 70-80 parts of ethylene-propylene-diene monomer rubber, 20-30 parts ofethylene-octene copolymer, 5-10 parts of dibenzoyl peroxide, 0.5-2 parts of maleic anhydride, 1-5 parts of KT-1, 1-5 parts of carbon nanotubes, 0.05-0.3 part of solid initiator, 0.05-0.3 part of liquid initiator, 0.1-0.5 part of thermal stabilizer, 0.1-0.3 part of antioxidant and 10-20 parts of nylon 6. The prepared nylon toughening agent belongs to injection molding-grade toughening agents, anda step of reaction-extrusion of nylon toughened by a toughening agent is omitted, so that the production cost is saved effectively; secondly, the toughening material can be used in an environment withan ambient temperature of 50 DEG C below zero, so that low-temperature resistance of the toughened nylon is improved effectively, and the notched impact strength of the toughened nylon is improved.