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A kind of bioactive polypeptide avpitptlnreq and its preparation method and application

A bioactive polypeptide and drug technology, applied in the field of protein, can solve problems such as human body risks, and achieve the effects of reducing body aging, improving quality, and good antioxidant activity

Active Publication Date: 2020-04-28
ZHEJIANG PANDA DAIRY GRP COMPANY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Some early synthetic antioxidants such as butyl hydroxyanisole (BHA) and 2,6-di-tert-butyl-4-methylphenol (BHT) were used in food as lipid antioxidants, but these artificial Synthetic additives are potentially dangerous to the human body

Method used

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  • A kind of bioactive polypeptide avpitptlnreq and its preparation method and application
  • A kind of bioactive polypeptide avpitptlnreq and its preparation method and application
  • A kind of bioactive polypeptide avpitptlnreq and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The artificial synthesis of embodiment 1 active peptide AVPITPTLNREQ

[0039] 1. Synthesis of Bioactive Peptides

[0040] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak in 50ml of dichloromethane (DCM).

[0041] After 2.2 hours, the resin was washed with nitrogen-dimethylformamide (DMF) 3 times the volume of the resin, and then drained. This was repeated four times, and the resin was drained for use.

[0042] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.

[0043] 4. Take a small amount of resin and use ninhydrin (Nine Wells Ninhydrin) method to test it (Two drops each of Test A and Test B, react at 100°C for 1 min), if the resin is colored, it means that the deprotection is successful.

[0044]...

Embodiment 2

[0080] Antioxidant activity experiment of embodiment 2 bioactive peptides

[0081] 1. Determination of in vitro antioxidant activity of biologically active peptide AVPITPTLNREQ by [DPPH·] method

[0082] 1. Experimental reagents and instruments:

[0083] Reagent: 1,1-diphenyl-2-trinitrophenylhydrazine (1,1-Diphenyl-2-picrylhydrazyl[DPPH·]), produced by Wako Company of Japan; Methanol, provided by Shanghai Sinopharm Company; obtained in Example 1 Milk-derived bioactive polypeptide AVPITPTLNREQ.

[0084] Main instruments: Sunrise microplate reader, product of Tecan Company, Austria; 96-well cell culture plate, manufactured by Millipore Company of the United States; analytical balance, product of Meitelei-tolido Company.

[0085] 2. Experimental method:

[0086] (1) 1mmol / L [DPPH·]methanol solution

[0087]Weigh 0.349 mg [DPPH·] with an analytical balance and dissolve it in 1 mL of methanol solution to prepare a 1 mmol / L [DPPH·] methanol solution, store it in the dark on tin ...

Embodiment 3

[0122] Anti-aging activity experiment of embodiment 3 bioactive peptides

[0123] 1. Experiment on the effect of biologically active polypeptide AVPITPTLNREQ on the reproductive ability of Caenorhabditis elegans

[0124] 1. Experimental reagents and instruments:

[0125] Reagents: Caenorhabditis elegans, Institute of Integrating Chinese and Western Medicine affiliated to Fudan University; Escherichia coli strain E.coli OP50, Institute of Integrating Chinese and Western Medicine affiliated to Fudan University; Agar powder, Sinopharm Chemical Reagent Co., Ltd.; Yeast powder, Sinopharm Chemical Reagent Co., Ltd. Company; the milk-derived biologically active polypeptide AVPITPTLNREQ obtained in Example 1.

[0126] Instruments and equipment: Likang RO15 pure water system, Likang Biomedical Technology Co., Ltd.; G136T Zealway intelligent high-temperature sterilization pot, Xiamen Zhiwei Instrument Technology Co., Ltd.; THZ-32 desktop constant temperature oscillator, Shanghai Precis...

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Abstract

The present invention relates to the field of proteins, and in particular relates to biologically active polypeptide AVPITPTLNREQ and a preparation method and application thereof, and the amino acid sequence of the biologically active polypeptide AVPITPTLNREQ is Ala-Val-Pro-Ile-Thr-Pro-Thr-Leu-Asn-Arg-Glu-Gln. In-vitro antioxidant experiments and in-vivo anti-aging experiments verify the biologically active polypeptide AVPITPTLNREQ has good antioxidant biological activity and anti-aging activity, on the one hand, the biologically active polypeptide AVPITPTLNREQ has good antioxidant activity, can remove free radicals in the body and improves the quality of life; on the other hand, the biologically active polypeptide AVPITPTLNREQ can enhance in-vivo anti-peroxidase system vitality, enhancesthe exogenous stimulus-resistant function of the body so as to reduce the probability of aging, senility and illness of the body, and has very important significance for development of food, health care products and drugs with anti-aging and antioxidant functions.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a biologically active polypeptide AVPITPTLNREQ and its preparation method and application. Background technique [0002] During the fermentation process of milk by lactic acid bacteria, a part of protein in milk is metabolized and utilized by lactic acid bacteria, and a series of physiological and biochemical reactions occur, making the protein into polypeptides or free amino acids, which are digested and absorbed by the human body or absorbed and transported by small intestinal epithelial cells directly into the blood circulation of the human body. Among these peptides, some have special physiological functions and are called "bioactive peptides". [0003] It is especially important to find safe bioactive peptides in natural food sources. In recent years, it has been found that some food-derived polypeptides have good biological activity, such as corn short peptides, soybean peptides, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C12N15/12A23L33/18A61K38/17A61K8/64A61P39/06A61Q19/08
CPCA23L33/18A23V2002/00A61K8/64A61K38/00A61Q19/08C07K14/4732A23V2250/55A23V2200/302
Inventor 张少辉李锡安林学海梁世排占东升
Owner ZHEJIANG PANDA DAIRY GRP COMPANY
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