Method for extracting zeaxanthin dipalmitate from liquid fructus lycii material

Abstract

The invention discloses a method for extracting zeaxanthin dipalmitate from a liquid fructus lycii material. Fresh fructus lycii and rehydrated fructus lycii are pulped, fructus lycii pulp is obtained, enzymolysis, liquid extraction and vacuum desolution are performed, a zeaxanthin dipalmitate extract is obtained, and different content of zeaxanthin dipalmitate micro-capsule powder is prepared andprocessed from the zeaxanthin dipalmitate extract. Dietary fiber, protein and syrup of fructus lycii are obtained from residues obtained after extraction of zeaxanthin dipalmitate respectively, and other fructus lycii functional ingredients such as flavone, polysaccharide, alkaloid and the like of fructus lycii are obtained through extraction. The extraction cost of fructus lycii functional ingredients is reduced, the raw material utilization rate of fructus lycii is increased, raw materials are utilized to the greatest extent, and the additional value of the raw materials is increased.

Description

Technical field:

[0001] The invention belongs to the field of biological extraction, and in particular relates to a method for extracting zeaxanthin dipalmitate from liquid wolfberry raw materials. technical background:

[0002] Goji berries are rich in carotenoids, goji berry flavonoids, goji berry polysaccharides, betaine, monosaccharides, disaccharides, riboflavin, taurine, unsaturated fatty acids, 18 kinds of amino acids and 19 kinds of essential trace elements. Carotenoids include zeaxanthin and its esters, phyllocarbin, cryptoxanthin, and β-carotene, among which zeaxanthin, which has the function of improving eyesight, is the highest among all fruits.

[0003] Regarding the deep processing and comprehensive utilization of Lycium barbarum, the disclosed technology is "Supercritical Lycium barbarum dregs extract and its production method" (CN 100400133C), the technical scheme adopts supercritical extraction technology, and the main component of the extract is Lycium barb...

Examples

Embodiment 1

[0052] The steps of extracting zeaxanthin dipalmitate from liquid wolfberry raw materials are as follows:

[0053] 1) Dried wolfberry or wolfberry residue is rehydrated by adding 5 times of water to beating, removing wolfberry seeds to obtain wolfberry pulp, adding pectinase and cellulase to the pulp for enzymatic hydrolysis, and then homogenizing under high pressure.

[0054] 2) Put the enzymatically hydrolyzed wolfberry pulp into the extraction system, add the extractant, seal the extraction, and keep the extraction pressure at 0.4-0.5Mpa. The extraction system can be set to reflux spray or stir; when the balance is reached, the extractant is recycled into the Decompression tank, decompression to precipitate the extract, recover the extractant, collect the zeaxanthin dipalmitate extract, after several stages of extraction, the wolfberry pulp that has been extracted is released from the bottom of the extraction tank. The extractants used are propane, butane, isobutane (R600a)...

Embodiment 2

[0070] The steps of extracting zeaxanthin dipalmitate from liquid wolfberry raw materials are as follows:

[0071] 2) Add 3.5 times of water to dry wolfberry or wolfberry residue for rehydration and beating, remove wolfberry seeds to obtain wolfberry pulp, add pectinase and cellulase to the pulp for enzymatic hydrolysis, and then homogenize under high pressure.

[0072] 2) Add the enzymatically hydrolyzed wolfberry pulp to the first extraction tank of the multi-stage countercurrent extraction system, add the extractant from the next stage (fresh extractant), turn on the reflux and stir for closed extraction, and keep the extraction pressure at 0.4—0.5Mpa, when the balance is reached, the extractant is recovered into the analysis tank, the extract is decompressed to precipitate, the extractant is recovered, the zeaxanthin dipalmitate extract is collected, and the pulp enters the next-level extraction tank, adding (Fresh extractant) extractant is extracted, and when the equilibr...

Embodiment 3

[0088] The steps of extracting zeaxanthin dipalmitate from liquid wolfberry raw materials are as follows:

[0089] 1) Lycium barbarum raw material pretreatment, fresh wolfberry was washed and beaten to remove wolfberry seeds, 30% whole wolfberry powder was added and dissolved to obtain wolfberry pulp, and pectinase and cellulase were added for enzymatic hydrolysis.

[0090] 2) Add the enzymatically hydrolyzed wolfberry pulp to the first extraction tank of the multi-stage countercurrent extraction system, add the extractant from the next stage (fresh extractant), turn on the reflux and stir for closed extraction, and keep the extraction pressure at 0.4—0.5Mpa, when the balance is reached, the extractant is recovered into the analysis tank, the extract is decompressed to precipitate, the extractant is recovered, the zeaxanthin dipalmitate extract is collected, and the pulp enters the next-level extraction tank, adding (Fresh extractant) extractant is extracted, and when the equi...

Technical field:

[0001] The invention belongs to the field of biological extraction, and in particular relates to a method for extracting zeaxanthin dipalmitate from liquid wolfberry raw materials. technical background:

[0002] Goji berries are rich in carotenoids, goji berry flavonoids, goji berry polysaccharides, betaine, monosaccharides, disaccharides, riboflavin, taurine, unsaturated fatty acids, 18 kinds of amino acids and 19 kinds of essential trace elements. Carotenoids include zeaxanthin and its esters, phyllocarbin, cryptoxanthin, and β-carotene, among which zeaxanthin, which has the function of improving eyesight, is the highest among all fruits.

[0003] Regarding the deep processing and comprehensive utilization of Lycium barbarum, the disclosed technology is "Supercritical Lycium barbarum dregs extract and its production method" (CN 100400133C), the technical scheme adopts supercritical extraction technology, and the main component of the extract is Lycium barb...

Examples

Embodiment 1

[0052] The steps of extracting zeaxanthin dipalmitate from liquid wolfberry raw materials are as follows:

[0053] 1) Dried wolfberry or wolfberry residue is rehydrated by adding 5 times of water to beating, removing wolfberry seeds to obtain wolfberry pulp, adding pectinase and cellulase to the pulp for enzymatic hydrolysis, and then homogenizing under high pressure.

[0054] 2) Put the enzymatically hydrolyzed wolfberry pulp into the extraction system, add the extractant, seal the extraction, and keep the extraction pressure at 0.4-0.5Mpa. The extraction system can be set to reflux spray or stir; when the balance is reached, the extractant is recycled into the Decompression tank, decompression to precipitate the extract, recover the extractant, collect the zeaxanthin dipalmitate extract, after several stages of extraction, the wolfberry pulp that has been extracted is released from the bottom of the extraction tank. The extractants used are propane, butane, isobutane (R600a)...

Embodiment 2

[0070] The steps of extracting zeaxanthin dipalmitate from liquid wolfberry raw materials are as follows:

[0071] 2) Add 3.5 times of water to dry wolfberry or wolfberry residue for rehydration and beating, remove wolfberry seeds to obtain wolfberry pulp, add pectinase and cellulase to the pulp for enzymatic hydrolysis, and then homogenize under high pressure.

[0072] 2) Add the enzymatically hydrolyzed wolfberry pulp to the first extraction tank of the multi-stage countercurrent extraction system, add the extractant from the next stage (fresh extractant), turn on the reflux and stir for closed extraction, and keep the extraction pressure at 0.4—0.5Mpa, when the balance is reached, the extractant is recovered into the analysis tank, the extract is decompressed to precipitate, the extractant is recovered, the zeaxanthin dipalmitate extract is collected, and the pulp enters the next-level extraction tank, adding (Fresh extractant) extractant is extracted, and when the equilibr...

Embodiment 3

[0088] The steps of extracting zeaxanthin dipalmitate from liquid wolfberry raw materials are as follows:

[0089] 1) Lycium barbarum raw material pretreatment, fresh wolfberry was washed and beaten to remove wolfberry seeds, 30% whole wolfberry powder was added and dissolved to obtain wolfberry pulp, and pectinase and cellulase were added for enzymatic hydrolysis.

[0090] 2) Add the enzymatically hydrolyzed wolfberry pulp to the first extraction tank of the multi-stage countercurrent extraction system, add the extractant from the next stage (fresh extractant), turn on the reflux and stir for closed extraction, and keep the extraction pressure at 0.4—0.5Mpa, when the balance is reached, the extractant is recovered into the analysis tank, the extract is decompressed to precipitate, the extractant is recovered, the zeaxanthin dipalmitate extract is collected, and the pulp enters the next-level extraction tank, adding (Fresh extractant) extractant is extracted, and when the equi...