Hollow platinum-copper-cobalt ternary alloy nanoparticle mimic enzyme and preparation and application thereof

A nanoparticle and cobalt alloy technology, which is applied in the field of catalytic materials and analytical chemistry, can solve the problems of complex synthesis and purification steps, limit the application of natural enzymes, inactivation and denaturation, etc., and achieve high chemical stability, high repeatability, and non-toxic reagents Effect

Active Publication Date: 2018-04-10
XUCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most natural enzymes are proteins, and their activity is easily inactivated and denatured by many factors such as temperature and chemical

Method used

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  • Hollow platinum-copper-cobalt ternary alloy nanoparticle mimic enzyme and preparation and application thereof
  • Hollow platinum-copper-cobalt ternary alloy nanoparticle mimic enzyme and preparation and application thereof
  • Hollow platinum-copper-cobalt ternary alloy nanoparticle mimic enzyme and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Preparation of platinum-copper-cobalt alloy nanoparticle mimic enzyme:

[0036] co 3 o 4 Preparation of granules: Put 0.6g cobalt acetate, 2.2g CTAB, 60ml ethylene glycol, and 11ml water in a 100ml beaker, stir the mixture for 20 minutes, then transfer the mixture to a 100ml polytetrafluoroethylene reactor for 48 hours at 180°C . The obtained black precipitate was washed twice with water and ethanol respectively, and then vacuum-dried at 60°C to obtain a black solid powder, which was calcined at 320°C for 10 minutes to obtain black Co 3 o 4 particles.

[0037] Take PVP, Glycine, g Co 3 o 4 、K 2 PtCl 4 , CuCl 2 、NiCl 2 , mixed with deionized water and ultrasonically dispersed for 5 minutes, and the mixed solution was transferred to a 20mL reactor at 200°C for 6 hours. After the reaction, the product was washed twice with ethanol and water respectively, and centrifuged at 10,000 rpm / min for 15-20 min. and finally dispersing the obtained alloy nanoparticles int...

Embodiment 2

[0046] The method for detecting glucose concentration, the process is as follows:

[0047] 1) Mix the platinum-copper-cobalt alloy nanoparticle mimetic enzyme prepared in Example 1 with the TMB solution, and record the absorbance of the characteristic absorption peak at 650 nm using the absorption curve of the solution with an ultraviolet-visible absorption spectrometer.

[0048] 2) Take 3.0ml of deionized water, add 200μl of 1mg / ml glucose oxidase (GOX), 0-400μl of 0.1M glucose aqueous solution to it in sequence, and then add 20μl of 20mM 3,3',5, 5'-Tetramethylbenzidine (TMB), 5 μl 1.3mM platinum-copper-cobalt alloy nanoparticles simulated enzyme solution, then mix the above solution evenly, place it at room temperature (25°C) for 15 minutes, and use a UV-visible spectrophotometer to detect Absorbance values. Draw a working curve by plotting the detected absorbance value at 650nm with the added glucose concentration.

[0049] Colorimetric detection of glucose based on plati...

Embodiment 3

[0053] The method for detecting the concentration of the hanging white block is as follows:

[0054] 1) Mix the platinum-copper-cobalt alloy nanoparticle imitative enzyme prepared in Example 2 with hydrogen peroxide and TMB solution, and use a UV-visible absorption spectrometer to scan the kinetic mode to record the absorption curve of the solution every 2 minutes, and record the characteristic absorption at 650nm The absorbance of the peak is used to monitor and calculate the oxidation rate of TMB through the change of absorbance with time.

[0055] 2) After mixing the platinum-copper-cobalt alloy nanoparticle mimic enzyme prepared in Example 2 with hydrogen peroxide and TMB solution, add 0.3, 0.5, 1.0, 2.0, 5.0, 10.0, 15.0, 20.0, 30.0, 50.0, 60.0 , 80.0, and 100 μM of the hanging white block solution, use the UV-visible absorption spectrometer to scan the kinetic mode to record the absorption curve of the solution every 2 minutes, record the absorbance of the characteristic ...

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Abstract

The invention discloses a hollow platinum-copper-cobalt ternary alloy nanoparticle mimic enzyme and a preparation and application thereof. In the mimic enzyme, nano-particles are of a hollow structure, the Pt/Cu/Co molar ratio in alloy composition is 1.33:1:1, and the average particle size is 32.2+/-5.1 nm. The preparation method includes the steps that aqueous solutions of potassium chloroplatinate, copper chloride and nickel chloride are mixed, then polyvinylpyrrolidone, glycine and Co3O4 are added, and stirring and ultrasonic treatment are carried out to make the powder fully dispersed; thetemperature is increased to 180-210 DEG C, a constant-temperature reaction is carried out for 6-10 hours, after the reaction is finished, centrifugal separation and washing are carried out to obtainthe alloy nanoparticles, and the alloy nanoparticles are dispersed into water to obtain a platinum-copper-cobalt alloy nanoparticle mimic enzyme solution. The obtained platinum-copper-cobalt alloy nanoparticle mimic enzyme has very high chemical stability under strong acid, strong base, high salinity and high temperature conditions, and has potential application value in the fields of immunoassay,biological detection, clinical diagnosis and the like as a novel mimic enzyme.

Description

technical field [0001] The invention belongs to the field of catalytic materials and analytical chemistry, and in particular relates to a platinum-copper-cobalt alloy nanoparticle imitation enzyme and its preparation method and application. Background technique [0002] Enzymes are a class of biopolymer substances with biocatalytic functions. With their high catalytic selectivity and high activity, they play an important role in biochemistry, food, chemical industry, agriculture, etc. However, most natural enzymes are proteins, and their activity is easily inactivated and denatured by interference from many factors such as temperature and chemical environment (such as pH value). In addition, complex synthesis and purification steps and high prices also limit the application of natural enzymes. Compared with natural enzymes, artificial simulated enzymes have simpler structures, stable chemical properties, catalytic functions similar to enzymes, and are cheap and easy to obta...

Claims

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Application Information

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IPC IPC(8): B01J23/89G01N21/31B22F9/24
CPCB01J23/8926B01J35/0013B01J35/006B22F9/24G01N21/31
Inventor 何伟伟蔡军辉张丽霞毛远洋贾会敏
Owner XUCHANG UNIV
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