Ginkgo fruit and ganoderma lucidum concentrated solution and preparation method thereof
A technology of Ganoderma lucidum concentrate and Ganoderma lucidum liquid, applied in the field of food science, can solve the problems of easy loss of nutrients and poor health care effect, and achieve the effects of enhancing health care function, nutrition and functionality
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Embodiment 1
[0036] Commercially available ganoderma lucidum fruit bodies were crushed through a 40-mesh sieve, and ganoderma lucidum was enzymatically hydrolyzed with cellulase (commercially available, SIGMA reagent, C1184, derived from Aspergillus niger), and the enzymatic hydrolysis conditions were changed to obtain an enzymatic hydrolysis solution. Centrifuge the enzymatic hydrolyzate at a speed of 2000r / min for 10min, and retain the supernatant. The soluble total sugar content of the enzymatic hydrolyzed supernatant of Ganoderma lucidum under different conditions was determined by sulfuric acid-phenol method, and the scavenging rate of DPPH free radicals by Ganoderma lucidum liquid indicated the antioxidant activity of Ganoderma lucidum hydrolyzate.
[0037] 1. Determination of soluble polysaccharide content by sulfuric acid-phenol method
[0038] (1) Drawing of standard curve
[0039] Preparation of 1mg / mL glucose standard solution: Accurately weigh 100mg of glucose, put it in a 100...
Embodiment 2
[0061] Commercially available ganoderma lucidum fruit bodies were crushed through a 40-mesh sieve, and ganoderma lucidum was enzymatically hydrolyzed with cellulase (commercially available, SIGMA reagent, C1184, derived from Aspergillus niger), and the enzymatic hydrolysis conditions were changed to obtain an enzymatic hydrolysis solution. Centrifuge the enzymatic hydrolyzate at a speed of 2000r / min for 10min, and retain the supernatant. The soluble total sugar content of the enzymatic hydrolyzed supernatant of Ganoderma lucidum under different conditions was determined by sulfuric acid-phenol method, and the scavenging rate of DPPH free radicals by Ganoderma lucidum liquid indicated the antioxidant activity of Ganoderma lucidum hydrolyzate.
[0062] Analytical methods such as figure 1 with figure 2 Described.
[0063] image 3 with Figure 4 The results showed that: with the increase of enzymatic hydrolysis time, the total soluble sugar content gradually increased and th...
Embodiment 3
[0065] After the commercially available ginkgo is peeled and germ removed, steamed and beaten, the amylase and pectinase compound enzymes are added to enzymatically hydrolyze under the conditions of different enzyme amounts (0.5%, 1%, 1.5%, 2%) , changing the conditions of enzymatic hydrolysis to obtain enzymatic hydrolysis solution. Centrifuge the enzymatic hydrolyzate at a speed of 2000r / min for 10min, and retain the supernatant. The soluble total sugar content of the ganoderma lucidum enzymatic hydrolysis supernatant was determined at 490 nm by the sulfuric acid-phenol method (see the description in Example 1 for the method).
[0066] Analysis method of phenolic acids in Ginkgo biloba extract:
[0067] Dissolve the weighed ginkgolic acid reference substance with anhydrous methanol, and constant volume to obtain a ginkgolic acid reference substance solution. Take an appropriate amount of this solution in a 1cm quartz cuvette, and measure the absorbance at 235nm with methan...
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