Lactic acid bacteria microbial inoculant applicable to myriophyllum quaticum silage, and preparation method and application of lactic acid bacterium inoculant

The technology of green foxtail algae and green foxtail algae is applied in the field of lactic acid bacteria inoculum and its preparation, and can solve the problems of low silage protein content, low utilization rate of silage raw materials, non-degradation of cellulose, etc., so as to improve the protein content. , Inhibit the growth of fungi such as mold, and reduce the effect of cellulose content

Active Publication Date: 2018-04-20
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the large-scale production of silage that uses crop straw as silage raw material is mainly to add yeast to ferment the silage raw material. This fermentation method takes a long time, usually takes 2-3 months, and the protein con

Method used

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  • Lactic acid bacteria microbial inoculant applicable to myriophyllum quaticum silage, and preparation method and application of lactic acid bacterium inoculant
  • Lactic acid bacteria microbial inoculant applicable to myriophyllum quaticum silage, and preparation method and application of lactic acid bacterium inoculant
  • Lactic acid bacteria microbial inoculant applicable to myriophyllum quaticum silage, and preparation method and application of lactic acid bacterium inoculant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation of embodiment 1 Lactococcus lactis inoculum

[0031] In the present embodiment, the preparation method of lactic acid bacteria inoculum comprises the following steps:

[0032] (1) Expanded culture of bacteria

[0033] Inoculate Lactococcus lactis in MRS liquid medium and culture at 37°C for 30 hours to obtain Lactococcus lactis liquid, then inoculate Lactococcus lactis liquid into new MRS liquid medium at a ratio of 1%, and inoculate at 37°C Expand the culture for 20 hours to obtain Lactococcus lactis culture fluid;

[0034] The composition of the MRS liquid medium is: 10.0g peptone, 10.0g beef extract, 5.0g yeast powder, 20.0g glucose, 5.0g sodium acetate, 1.0g Tween-80, 0.1g magnesium sulfate, 0.05g sulfate Manganese, 2.0g diammonium hydrogen citrate, 2.0g dipotassium hydrogen phosphate, 1000mL, sterilized for later use.

[0035] (2) Preparation of Lactococcus lactis bacterial agent

[0036] The obtained Lactococcus lactis culture solution was inoc...

Embodiment 2

[0038] The preparation of embodiment 2 Lactococcus lactis inoculum

[0039] In the present embodiment, the preparation method of lactic acid bacteria inoculum comprises the following steps:

[0040] (1) Expanded culture of bacteria

[0041] Inoculate Lactococcus lactis in MRS liquid medium and culture at 37°C for 26 hours to obtain Lactococcus lactis bacteria liquid, then inoculate Lactococcus lactis bacteria liquid into new MRS liquid medium at a ratio of 1.5%, at 37°C Expand the culture for 24 hours to obtain Lactococcus lactis culture fluid;

[0042] The composition of described MRS liquid medium is: 11.0g peptone, 11.0g beef extract, 6.0g yeast powder, 21.0g glucose, 6.0g sodium acetate, 1.5g Tween-80, 0.15g magnesium sulfate, 0.08g sulfuric acid Manganese, 3.0g diammonium hydrogen citrate, 3.0g dipotassium hydrogen phosphate, 1000mL, sterilized for later use.

[0043] (2) Preparation of Lactococcus lactis bacterial agent

[0044] The obtained Lactococcus lactis cultur...

Embodiment 3

[0046] The preparation of embodiment 3 Lactobacillus plantarum inoculum

[0047] In the present embodiment, the preparation method of Lactobacillus plantarum inoculum comprises the following steps:

[0048] (1) Expanded culture of bacteria

[0049] Inoculate Lactobacillus plantarum in MRS liquid medium and culture at 37°C for 30 hours to obtain Lactobacillus plantarum liquid, then inoculate the Lactobacillus plantarum liquid into new MRS liquid medium at a ratio of 1%, and incubate at 37°C Expand the culture for 20 hours to obtain the culture solution of Lactobacillus plantarum;

[0050] The composition of the MRS liquid medium is: 10.0g peptone, 10.0g beef extract, 5.0g yeast powder, 20.0g glucose, 5.0g sodium acetate, 1.0g Tween-80, 0.1g magnesium sulfate, 0.05g sulfate Manganese, 2.0g diammonium hydrogen citrate, 2.0g dipotassium hydrogen phosphate, 1000mL, sterilized for later use.

[0051] (2) Preparation of Lactobacillus plantarum inoculum

[0052] The obtained Lacto...

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Abstract

The invention discloses a lactic acid bacteria microbial inoculant applicable to myriophyllum quaticum silage, and a preparation method and application of the lactic acid bacteria microbial inoculant.The lactic acid bacteria microbial inoculant is prepared from lactic acid bacteria and myriophyllum quaticum juice, wherein the content of lactic acid bacteria in the lactic acid bacteria microbial inoculant is 1.7*10<7>-8.4*10<7>CFU/g, and the lactic acid bacteria are selected from a group comprising lactococcus lactis, lactobacillus plantarum and a composite microbial inoculant obtained by mixing lactococcus lactis and lactobacillus plantarum. According to the preparation method, the lactic acid bacteria are cultured and domesticated by utilizing juice of myriophyllum quaticum, and the lactic acid bacteria microbial inoculant is used for fermenting the myriophyllum quaticum silage so as to prepare the myriophyllum quaticum silage. By adopting the lactic acid bacteria microbial inoculant, the pH value, the content of butyric acid and the content of neutral detergent fibers of the myriophyllum quaticum silage can be reduced, while the content of crude proteins and the content of generated lactic acid are increased, so that the fermentation quality and nutritional value of the myriophyllum quaticum silage can be improved, and data reference is provided to resource utilization of the myriophyllum quaticum and popularization and application of the myriophyllum quaticum in culture production.

Description

technical field [0001] The invention relates to the technical field of silage processing, in particular to a lactic acid bacteria agent suitable for silage silage and its preparation method and application. Background technique [0002] In recent years, my country's aquaculture industry has developed rapidly, and there is a current situation of "three shortages and one deficiency" in feed resources, especially the serious shortage of protein feed raw materials. By 2025, my country's feed consumption will reach 250 million tons, accounting for more than 42% of the total grain consumption . But at present, my country's protein feed self-sufficiency rate is less than 50%, of which 70-80% of fish meal and soybeans are dependent on imports, and the import of feed corn is also increasing year by year [Wang Shouwei. The development status and trend of my country's meat industry [J]. North Animal Husbandry Industry, 2017(10): 10-10; Wu Shiqiao, Zhang Shiyuan, Qi Ruxin. Research progr...

Claims

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Application Information

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IPC IPC(8): A23K30/18C12N1/20C12R1/46C12R1/25
CPCA23K30/18C12N1/20A23V2400/231A23V2400/169
Inventor 徐圣君庄绪亮孙茜白志辉庄国强
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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