Method for forming hydrogel and wrapping medicine through cleaving polypeptide with thrombin
A technology of thrombin and hydrogel, which is applied in the field of biomaterials to achieve the effect of good repeatability and simple operation methods
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Embodiment 1
[0036] The method of the present invention adopts the conventional solid-phase Fmoc method, that is, the monomer amino acid protected by Fmoc on the solid-phase resin is deprotected to expose the amino group, and forms a peptide bond with the carboxyl group of the amino acid in the solution through a condensation reaction, thereby linking the amino acid to the On the resin, the peptide chain is extended from the C-terminus to the N-terminus.
[0037] 1. Basic materials:
[0038] (1) Resin and linking molecule: The resin selected by the solid-phase Fmoc method is Rink Amide- resin. This kind of resin has very good swelling property, which can make the condensation reaction between the peptide chains better, and there is enough network space to meet the growing peptide chains. HBTU and HOBt are used as linking molecules to immobilize the polypeptide molecules on the resin.
[0039] (2) Monomer: The monomer used in the synthesis is a chemically modified α-amino acid.
[0040...
Embodiment 2
[0055] For the polypeptide hydrogel obtained by the method in Example 1, absorb 500 microliters of PBS buffer solution with a pH of 7.4 and a salt ion concentration of 0.01M on the upper layer of the hydrogel, then place it in a water bath at 37°C, and take out the release solution after 0.5h , and add 500 microliters of new PBS buffer solution under the same conditions, and replace the buffer solution sequentially after 1h, 2h, 4h.... Then, the absorbance of the release liquid was measured by a UV-visible spectrophotometer, and the amount of doxorubicin hydrochloride was calculated according to Lambert-Beer's law, and the calculated content was added to obtain the total release amount. And draw the total release amount-time graph according to the relationship between the total release amount and time. In addition, the PBS buffer conditions were selected as pH 5.5 0.01M and pH 6.5 0.01M, and the doxorubicin hydrochloride in the polypeptide hydrogel was measured at 37°C The rel...
Embodiment 3
[0057] Select the PBS buffer condition as: pH7.4 0.01M, and measure the relationship between the release amount of doxorubicin hydrochloride and time in the polypeptide hydrogel at 4°C, 37°C and 60°C respectively. The steps are the same as in Example 2, and the results are as follows image 3 .
[0058] It can be seen from the above three examples that the synthesis of the polypeptide hydrogel encapsulating the drug in the present invention is very convenient and its composition is controllable. And the encapsulated drug shows different drug release kinetics with the change of pH and temperature. Both temperature and pH have a certain influence on drug release. The lower the pH, the faster the drug release, and the more the drug is released in the same time. The higher the temperature, the faster the release and the greater the amount. It shows that it can be effectively applied to the controlled release of drugs.
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