Biocontrol bacterium Meyerozyma guilliermondii Y-1 for controlling apple ring rot and biocontrol preparation thereof

A technology for biocontrol fungicides and ring disease, applied in biocides, fungicides, biocides, etc., can solve the problems of high pesticide residues, high cost, and difficulty in fruit ring disease prevention, and achieve environmental protection, The effect of maintaining ecological balance and stabilizing the control effect

Active Publication Date: 2018-04-20
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the problems of difficult prevention and control of fruit ring disease, the main disease of apple production and storage period in current agricultural production, high cost, increasingly prominent resistance to chemical pesticides, high pesticide residues, etc., screens out a high-efficiency biocontrol strain and prepares a biocontrol agent , effective control of apple fruit ring spot

Method used

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  • Biocontrol bacterium Meyerozyma guilliermondii Y-1 for controlling apple ring rot and biocontrol preparation thereof
  • Biocontrol bacterium Meyerozyma guilliermondii Y-1 for controlling apple ring rot and biocontrol preparation thereof
  • Biocontrol bacterium Meyerozyma guilliermondii Y-1 for controlling apple ring rot and biocontrol preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Isolation and strain identification of Pichia pastoris (Meyerozymaguilliermondii) Y-1 described in Example 1

[0026] 1.1 Isolation and screening of strains

[0027] Mature healthy grapes were collected from commercialized vineyards in Yantai, Shandong, and the strains were isolated from the surface of the grapes, using the conventional gradient dilution coating separation method, using PDA medium (containing streptomycin sulfate) and YPD agar medium (containing streptomycin sulfate) They were cultured at 28°C respectively, and the colonies with obvious differences in colony morphology were picked, purified and preserved on YPD agar medium, and the primary screening and multiple re-screening of antagonistic bacteria were carried out with Apple ringworm as the target pathogen, and finally a An antagonistic yeast strain with strong activity was named Y-1.

[0028] The above-mentioned PDA medium used has the following components and formula: weigh 200g after peeling the p...

Embodiment 2

[0035] Example 2 The inhibitory effect of Pichia pastoris (Meyerozyma guilliermondii) Y-1 on various plant pathogenic fungi

[0036] Different concentrations of Pichia guilliermondii (Meyerozyma guilliermondii) Y-1 cells had significant inhibitory effects on apple ringworm, and PDA was added with 10 7 cfu mL -1 The most significant inhibitory effect was strain Y-1 ( image 3 ). In addition, the Pichia pastoris Y-1 strains are effective against fruit and vegetable gray mold, fruit and vegetable penicillium, apple tree rot, apple anthracnose, apple mold core, apple fruit spot, peach brown rot, grape white The main fruit and vegetable pathogenic fungi such as rot fungus and grape anthracnose fungus all had significant inhibitory effect and showed good broad-spectrum antibacterial properties. The results are shown in Table 1.

[0037] Table 1 Inhibitory effect of Pichia guilliermondii Y-1 on the main pathogenic fungi of fruits and vegetables

[0038]

[0039]

[0040] N...

Embodiment 3

[0042] Example 3: Control effect of Pichia pastoris (Meyerozyma guilliermondii) Y-1 cell culture fluid on apple ring spot

[0043] Inoculate the Y-1 seed fermentation liquid of Pichia guilliermondii into YPD liquid medium, and culture it with constant temperature and shaking at 30°C for 48 hours to obtain the cell culture liquid of the Y-1 strain, and adjust the concentration to 10 7 cfu mL -1 . Apple ringworm was activated and cultivated on PDA for 3 days, and a bacterium cake with a diameter of 5 mm was taken from the edge of the colony for subsequent use. Choose healthy Fuji apples of uniform size, disinfect the surface with 75% alcohol, and dry them at room temperature. At the equator of the apples, use a sterile punch to make 3 wounds, each with a diameter of 3 mm and a depth of 1 mm. Inoculate 20 μL of Pichia mongolica Y-1 cell culture solution into each wound, inoculate the above-mentioned activated ringworm bacteria cake after drying at room temperature, and inoculat...

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Abstract

The invention relates to a biocontrol strain Y-1 for controlling apple ring rot and application thereof to control of the apple ring rot, and belongs to the technical field of plant disease and pest biocontrol. The strain is Meyerozyma guilliermondii Y-1, and the culture collection number is CCTCC NO: M2015814. The Meyerozyma guilliermondii Y-1 is used for preparing a biocontrol preparation for controlling the apple ring rot, and the biocontrol preparation has a significant effect of controlling a variety of pathogenic fungi. An active component, namely the Meyerozyma guilliermondii Y-1, of the biocontrol preparation provided by the invention has the characteristics of a stable control effect, a long persistent effect, a wide disease control spectrum, environment friendliness and the like.The biocontrol strain Y-1 breeding speed is high, a culture condition is simple, collection and industrial production are easy, and development and application prospects are good.

Description

technical field [0001] The invention relates to the technical field of biological control of plant diseases, in particular to a biocontrol fungus Pichia mongolica Y-1 for preventing and treating apple fruit ring disease and a biocontrol agent thereof. Background technique [0002] Apples are rich in carbohydrates, vitamins and inorganic salts, and are an important source of nutrition and material basis for people. As an important economic crop and export fruit in my country, apples have the largest planting area and output in the world. However, the export rate of apples is less than 3% of the total output, and the market share in the world is extremely disproportionate to my country's status as a major apple producing country. , The disease during storage is an important factor causing this phenomenon, and the disease caused by fungal infection in the post-harvest link of apples has brought huge losses to people. [0003] Apple fruit ring disease caused by Botryosphaeria do...

Claims

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Application Information

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IPC IPC(8): C12N1/16A01N63/04A01P3/00C12R1/645
CPCA01N63/30C12N1/16C12N1/145C12R2001/645
Inventor 王彩霞孟璐璐张清明练森李保华
Owner QINGDAO AGRI UNIV
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