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A thermophilic Pb ore-forming bacterium and its method for passivating Pb in sludge high-temperature composting

A technology for high-temperature composting and Thermus thermophilus, which is applied to sewage/sludge fertilizers, microorganism-based methods, biochemical equipment and methods, etc. Temperature, the effect of shortening the fermentation cycle

Active Publication Date: 2021-02-02
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently reported lead ore-forming bacteria are all normal temperature bacteria (optimum growth temperature <40°C), which cannot grow and reproduce in high temperature environments such as composting or ultra-high temperature composting.
So far, there is no research report on the synergistic promotion of Pb passivation in sludge high-temperature composting by thermophilic lead ore-forming bacteria and the mineralization of thermophilic microorganisms

Method used

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  • A thermophilic Pb ore-forming bacterium and its method for passivating Pb in sludge high-temperature composting
  • A thermophilic Pb ore-forming bacterium and its method for passivating Pb in sludge high-temperature composting
  • A thermophilic Pb ore-forming bacterium and its method for passivating Pb in sludge high-temperature composting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 FAFU013 strain screening and identification

[0024] (1) Screening of FAFU013 strain

[0025] Weigh 5g of sludge compost samples in the high temperature period (<70°C) into a conical flask filled with 15 glass beads and 50mL of sterile water, place on a shaker and vibrate at 180rpm for 1 hour to fully disperse the samples, and then place them at 75°C Next, let stand for 3 hours.

[0026] Pipette 500 μL of high-temperature enrichment solution and low-fold dilution solution, and spread evenly on the improved YTPG solid medium. Put the evenly coated plate upside down into a sealed bag, and place a petri dish filled with distilled water at the same time, seal it and place it in a 75°C incubator for cultivation. After the colonies grow, carefully observe the characteristics of the colonies to determine the different types of strains, and separate and purify by streaking on the good YTPG solid plate medium until the pure colonies of FAFU013 are obtained. Transfer...

Embodiment 2

[0032] Example 2 Tolerance of FAFU013 strain to lead and its mediated mineralization of lead

[0033] (1) Pick colonies from the well-preserved Thermus thermophilus FAFU013 plate medium, inoculate into DSMZ Medium 74 liquid medium, and activate and cultivate for 16 hours at 75°C and 180 rpm. The activated Thermus thermophilus FAFU013 was inoculated to contain 50mg / L, 100mg / L, 200mg / L, 300mg / L, 400mg / L Pb(II) (Pb(NO 3 ) 2 ) in DSMZ Medium 74 liquid medium, cultured at 75°C and 180 rpm. By detecting the OD600 value of the bacterial solution, the influence of different lead ion concentrations on the growth of Thermus thermophilus FAFU013 was determined. The result is as figure 1 As shown, the results show that 400mg / L Pb(II) has no effect on the growth of Thermus thermophilus FAFU013.

[0034](2) After culturing for 48 hours, centrifuge at 12,000 rpm for 10 minutes, and take the supernatant to measure the remaining lead content. The cells obtained after centrifugation were w...

Embodiment 3

[0035] Embodiment 3 FAFU013 mediates the mineralization of lead ions in lead-containing wastewater

[0036] (1) Take lead-containing wastewater from an enterprise in Fujian (the basic properties of the water sample are shown in Table 1), and adjust the pH of the wastewater to 5.5-6.5.

[0037] Table 1. Basic properties of lead-containing wastewater tested

[0038]

[0039] (2) The activated Thermus thermophilus FAFU013 was inoculated into DSMZ Medium 74 liquid medium at 1%, cultured at 75° C. and 180 rpm for 48 hours, and the cells were collected by centrifugation.

[0040] (3) According to the dosage of 10g / L (wet weight), add the cultured FAFU013 bacteria to the lead-containing wastewater, and act for 24 hours at 70°C with a stirring speed of 180-220 rpm. (AAS) was used to determine the lead content absorbed by FAFU013 cells, and the transformation of lead-containing minerals mediated by FAFU013 cells was determined by XRD.

[0041] The results showed that Thermus therm...

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Abstract

The invention discloses a thermophilic Pb ore-forming bacterial strain Thermus thermophilus FAFU013, and the preservation number is CGMCC No.14654. The thermophilic Pb mineral-forming bacterium FAFU013 provided by the present invention can induce Pb(II) to be converted into the most stable and intolerant pyrophyllite [Pb 5 (PO 4 ) 3 Cl, Ksp=10 ‑84.4 ], not only can effectively passivate the heavy metal lead in the sludge, reduce the bioavailability of Pb (II), but also maximize the use of the original nutrients of the sludge. In addition, the Pb ore-forming bacteria FAFU013 provided by the present invention can also use the nutrients in the sludge to grow and reproduce at 70-80°C, has good thermal stability, and its metabolic activity is not inhibited by high-temperature composting temperatures, which is beneficial to improving and Maintain the temperature of the high-temperature compost heap, promote the degradation of organic matter and the killing of pathogenic microorganisms, and shorten the fermentation cycle.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a thermophilic Pb ore-forming bacterium and a method for synergistically passivating lead in sludge high-temperature composting. Background technique [0002] Sludge is an inevitable sediment produced in the process of urban domestic sewage treatment. The annual sludge production in my country is increasing at a rate of 10-15% per year. By 2016, the annual production of wet sludge has exceeded 40 million tons. These increasingly large and highly concentrated sludge have long plagued the healthy development of my country's sewage treatment industry, and have also caused great safety hazards, environmental pressure and economic burden. On the other hand, sludge is rich in nutrients such as N and P and organic matter, and is an organic solid waste with great agricultural resource value. Sludge can be used as organic fertilizer after composting to impro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F15/00C05F17/20C12R1/01
CPCC05F7/00C05F17/00C12N1/205C12R2001/01C05F11/08Y02W30/40
Inventor 陈志周顺桂赵子强
Owner FUJIAN AGRI & FORESTRY UNIV
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