Preparation method of surface-enhanced Raman substrate and application of the substrate to detection of animal viruses

A surface-enhanced Raman and Raman substrate technology, applied in the field of Raman spectroscopy and nanomaterials, can solve the problems of different SERS substrate performance, irregular surface, poor signal enhancement effect and reproducibility, etc., to achieve enhanced Raman Effective, low cost, good reproducibility

Active Publication Date: 2020-05-26
HUAZHONG AGRI UNIV
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Problems solved by technology

[0004] Different SERS substrates have different properties, and the enhancement effect caused by the action of the analyte is also different. Currently, commonly used solid-phase SERS substrates (such as electrochemical rough electrode SERS substrates, noble metal sol SERS substrates, metal island film SERS substrates) , chemically deposited metal nanoparticle SERS substrate, nanoparticle assembly SERS substrate, etc.), the surface is irregular, which makes the signal enhancement effect and reproducibility poor

Method used

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  • Preparation method of surface-enhanced Raman substrate and application of the substrate to detection of animal viruses
  • Preparation method of surface-enhanced Raman substrate and application of the substrate to detection of animal viruses
  • Preparation method of surface-enhanced Raman substrate and application of the substrate to detection of animal viruses

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preparation example Construction

[0029] see figure 1 As shown, the embodiment of the present invention provides a method for preparing a surface-enhanced Raman substrate, comprising the following steps:

[0030] S1. Cut the ultra-pure aluminum into 2cm×2cm sheets, flatten it with a tablet machine, anneal it under the protection of high-purity nitrogen at a temperature of 500°C for 3-5 hours, and then put it into acetone for ultrasonic degreasing After 20 minutes, soak in NaOH solution with a concentration of 1M for a while, take it out, rinse it with ultrapure water, and dry it. (In this step, the size of the ultrapure aluminum sheet, the concentration of NaOH, the annealing temperature and the time can all be selected according to actual needs.)

[0031] S2. Use ordinary aluminum sheet as cathode and pretreated ultra-pure aluminum sheet as anode, seal one side of ordinary aluminum sheet and ultra-pure aluminum sheet and put it into the electrolytic cell, use magnetic stirring, and the oxidation voltage is 1...

Embodiment 2

[0054] S201. Use ordinary aluminum sheets as cathodes and pretreated ultra-pure aluminum sheets as anodes, seal one side of ordinary aluminum sheets and ultra-pure aluminum sheets and put them into the electrolytic cell, use magnetic stirring, and use oxalic acid with a concentration of 0.1M The solution is used as an electrolyte, oxidized for 6 hours under the conditions of oxidation voltage of 30V, oxidation current of 20mA, and temperature of 5°C, and then placed in a temperature of 40°C, containing CrO at a concentration of 3wt%. 3 and a concentration of 6% H 3 PO 4 The oxide layer was removed by immersing in the mixed solution for 10 minutes to obtain a preliminary porous structure template.

[0055] S202. Use the preliminary porous structure template as an anode, put it into an oxalic acid solution with a concentration of 0.1M, oxidize it for 20 minutes under the conditions of an oxidation voltage of 30V and a temperature of -5°C, and put it into H at a temperature of 2...

Embodiment 3

[0059] S301. Use ordinary aluminum sheets as cathodes and pretreated ultra-pure aluminum sheets as anodes, seal one side of ordinary aluminum sheets and ultra-pure aluminum sheets and put them into the electrolytic cell, use magnetic stirring, and use sulfuric acid with a concentration of 0.5M The solution is used as an electrolyte, oxidized for 3 hours under the conditions of oxidation voltage of 190V, oxidation current of 3mA, and temperature of 0°C, and then placed in a temperature of 70°C, containing CrO with a concentration of 1wt%. 3 and a concentration of 6% H 3 PO 4 soaked in the mixed solution for 40 minutes to remove the oxide layer and obtain a preliminary porous structure template.

[0060] S302. Use the preliminary porous structure template as an anode, put it into a sulfuric acid solution with a concentration of 0.5M, oxidize it for 20 minutes under the conditions of an oxidation voltage of 150V and a temperature of 10°C, and put it into H at a temperature of 20...

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Abstract

The invention discloses a preparation method of a surface-enhanced Raman substrate and the substrate for detecting animal virus, and relates to the technical field of Raman spectrum technologies and nanomaterials. The preparation method comprises the following steps: ultrapure aluminum is used as an anode, and general aluminum is used as a cathode for at least once of anodic oxidation to obtain porous anodic oxidation aluminum; silanization is performed on the porous anodic oxidation aluminum after surface hydrophilization to obtain a flexible composite film; and noble metal X is deposited onthe surface of the flexible composite film to obtain the surface-enhanced Raman substrate. The method is simpler, lower in cost, better in stability and excellent in repeatability, and can realize large-scale continuous preparation; and when the substrate is used for Raman detection, enhancement factors are high, reproducibility is excellent, stability is high, and a prominent surface-enhanced Raman effect is achieved on analyte with different concentrations.

Description

technical field [0001] The invention relates to the technical fields of Raman spectroscopy and nanomaterials, in particular to a method for preparing a surface-enhanced Raman substrate and the substrate is used for detecting animal viruses. Background technique [0002] Surface-enhanced Raman spectroscopy (SERS) is a highly sensitive analysis and detection technology that can provide molecular vibrational fingerprints and Raman signals that are positively correlated with the number of scattered molecules. Small, non-destructive in situ and other advantages, it has been widely used in public safety, food safety, environmental monitoring and other fields. [0003] When SERS is used for detection, the analyte is usually adsorbed on the SERS substrate, and the analyte is electromagnetically enhanced due to the excitation of surface localized plasmons. At the same time, the atomic clusters on the SERS substrate and the molecules adsorbed on it form a pull Man-enhanced active sit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C25D11/12C25D11/24C23C14/34C23C14/18G01N21/65G01N33/569
CPCC23C14/185C23C14/34C25D11/045C25D11/12C25D11/24G01N21/65G01N21/658G01N33/56983G01N2021/655G01N2333/17
Inventor 韩鹤友李晋杰吴龙殷文敏吕志成
Owner HUAZHONG AGRI UNIV
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