Clean extraction method of tea saponin

Abstract

The invention provides a clean extraction method of tea saponin. The clean extraction method comprises the following steps: (1) crushing a cake of camellia oleifera, sieving, wherein the fineness is 200 to 400 meshes; (2) adding normal hexane into the cake powder of camellia oleifera according to a material-to-liquid ratio of 1:(2 to 3), heating to 60 to 70 DEG C, refluxing for 1 to 3 h, degreasing, and drying; (3) adding ethanol into the cake powder of camellia oleifera according to the material-to-liquid ratio of 1:(15 to 25), heating to 50 to 70 DEG C, and performing ultrasonic-microwave synergistic extraction; (4) suction filtering and centrifuging the extracted solution, and obtaining tea saponin extract solution; (5) drying the extract solution, and obtaining a tea saponin crude product; and (6) dissolving the tea saponin crude product, successively filtering by virtue of a hyperfiltration membrane and an organic nano filtration membrane, drying, and preparing high-purity tea saponin. By adopting the clean extraction method, the utilization rate of raw materials is increased, the extraction rate of the tea saponin can be maximally increased, the extraction time is shortened,and compared with the traditional separation process, the clean extraction method has the advantages of low energy consumption, single-level separation efficiency, simple device, no phase change, no pollution and the like.

Description

technical field [0001] The invention belongs to the field of chemical and pharmaceutical raw materials with multiple uses, and in particular relates to a method for cleaning and extracting tea saponin. Background technique [0002] my country is the country with the highest yield of camellia oleifera in the world, the largest cultivation area and the richest varieties, mainly distributed in Hunan, Hubei, Guangdong, Guangxi, Fujian, Jiangxi and other provinces. The area of ​​camellia oleifera in my country is about 40,000km2. According to incomplete statistics, the country produces about 650kt of tea seeds every year, and the tea seed cake after oil extraction is more than 400kt. In the past, it was mostly used as fuel or fertilizer, and was also discarded as waste. The main components of tea seed cake are 5%-8% of residual tea oil, about 10.6% of tea saponin, and about 10.2% of protein. Tea saponin, also known as tea saponin, is a natural non-ionic surfactant, a pentacyclic...

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Problems solved by technology

At present, my country's industrialized production of tea saponin mainly adopts organic solvent extraction or water extraction. Although water extraction is low in cost and simple in operation, it takes a long time to extract, easily hydrolyzes tea saponin, and is relatively difficult to concentrate. The obtained tea saponin product is darker in color, There are many impurities, and subsequent purification is more difficult; the product obtained by alcohol extraction has higher quality, lighter color, and higher yield, but there are safety problems and the extraction time is relatively long

In terms of tea saponin purification, membrane separation and macroporous resin are commonly used tea saponin purification methods. Although these two methods obtain high product quality, the product yield is low and the investment in equipment and raw materials is large. Membrane and resin utilization The extraction rate is low, it is easy to cause blockage, and the follow-up work is relatively complicated; the purification process of the extraction method is long, consumes a lot of organic reagents, and the yield is low; the purity of the tea saponin product obtained by silica gel column chromatography is relatively high, but the yield is very low, so it is only suitable for Made in small quantities in the laboratory

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