The production process of abamectin

A production process and seed technology, which is applied in the field of abamectin fermentation production, can solve the problems of poor oxygen dissolution in the medium, high wastewater COD, low starch utilization rate, etc., achieve significant production advantages, reduce wastewater COD, improve dissolution The effect of oxygen

Active Publication Date: 2021-08-10
TIANJUSHI ENG TECH GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the viscous starch feed liquid, the effect of dissolved oxygen in the medium will be poor. Generally, under the requirement of an average level of 6g / L for the production tank unit, the tank release coefficient is generally lower than 75%; and the starch can only be converted into glucose first. It can be used by microorganisms, but other sugars will be produced during the fermentation process, which not only makes the utilization rate of starch low, but also needs to add toluene to remove various sugars produced by starch metabolism in the extraction process after fermentation, so that the fermentation produces The wastewater COD is very high, which puts pressure on environmental protection

Method used

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  • The production process of abamectin

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Embodiment 1

[0019] Present embodiment relates to a kind of production technology of Abamectin.

Embodiment 11

[0021] The production of Abamectin comprises the following steps:

[0022] a, shaking flask seed culture: by weight percentage with glucose 1.5%, corn steep liquor 0.3%, yeast extract 0.5%, calcium carbonate 0.1%, the remainder is tap water, and adjust pH7.0 configuration seed culture medium, after sterilizing and cooling , inoculate the grown Streptomyces avermitilis spores in the seed medium under aseptic conditions, and culture and grow the shake flask at 28±1°C for 24 hours to obtain the shake flask seed liquid;

[0023] B, seed tank cultivation: inoculate the shake flask seed liquid of step a into the seed tank, by weight percentage with glucose 2%, corn steep liquor 0.8%, yeast extract 0.3%, calcium carbonate 0.2%, surplus is tap water, and adjusts pH7 .2 Configure the seed medium in the seed tank, the inoculation amount is 0.1% of the volume of the seed medium, cultivate and grow at 28±1°C for 24-28 hours to obtain the seed liquid;

[0024] C, fermentation tank culture...

Embodiment 12

[0028] The production of Abamectin comprises the following steps:

[0029] a, shaking flask seed culture: by weight percentage with glucose 2%, corn steep liquor 0.5%, yeast extract 0.5%, calcium carbonate 0.1%, the remainder is tap water, and adjust pH7.0 configuration seed culture medium, after sterilizing and cooling , inoculate the grown Streptomyces avermitilis spores in the seed medium under aseptic conditions, and culture and grow the shake flask at 28±1°C for 28 hours to obtain the shake flask seed liquid;

[0030] B, seed tank cultivation: inoculate the shake flask seed solution of step a into the seed tank, use glucose 2% by weight percentage, corn steep liquor 0.5%, yeast extract 0.5%, calcium carbonate 0.2%, surplus is tap water, and adjust pH7 .2 Configure the seed medium in the seed tank, the inoculation amount is 0.1% of the volume of the seed medium, and cultivate and grow at 28±1°C for 24-28 hours to obtain the seed liquid;

[0031] C, fermentation tank cultu...

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Abstract

The invention discloses a production process of abamectin, comprising the steps of shaking flask seed cultivation, seed tank cultivation, and fermenter tank cultivation, seed culture medium is used in the shaking flask seed cultivation and seed tank cultivation steps, and the The carbon source of the seed culture medium is glucose; the fermented medium is used in the fermentor culture step, and the carbon source of the fermented medium is glucose and sorbitol; the carbon source is supplemented in the fed-batch mode in the fermentor culture step , the carbon source is glucose and sorbitol. The obtained production process can effectively improve the dissolved oxygen in the fermentation process, reduce the power cost of production; and avoid the feedback inhibition of microbial metabolism caused by adding too much glucose, so as to ensure the improvement of the fermentation unit when putting the tank; it can also reduce the post-fermentation The sugar removal step in the extraction stage significantly reduces the COD of the wastewater produced by fermentation.

Description

technical field [0001] The invention relates to the technical field of abamectin fermentation production, in particular to a production process of abamectin. Background technique [0002] Avermectins are a group of 16-membered macrolide antibiotics with insecticidal, acaricidal and nematicidal activities, which are fermented by Streptomyces avermitilis in Streptomyces griseus. Natural abamectin contains 8 homologue components with similar structures, the main 4 species are A1a, A2a, B1a and B2a, and their total content is ≥80%; the corresponding 4 homologues with smaller proportions are A1b, A2b, B1b and B2b, the total content of which is ≤20%. Abamectin is widely used in agriculture and is a common pesticide preparation. It is used as an insecticide to control Brassicaceae diamondback moth, cotton bollworm, mites, cabbage caterpillar, etc. [0003] At present, the basal medium used for fermentation and production of abamectin uses high-concentration starch as the carbon s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/62C12N1/20C12R1/465
CPCC12N1/20C12P19/623
Inventor 赵少欣杨玉淮陈平马志民张欣巧田林生李兰芳剧盼盼孟翔凯
Owner TIANJUSHI ENG TECH GROUP
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