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Method for extending shelf life of fishery liquid lactobacillus plantarum product

A plant lactobacillus, shelf life technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of large packaging containers, inconvenient transportation, cumbersome fixing methods, and expensive products, so as to ensure the liveness of the bacteria liquid Bacterial count, beneficial to storage and transportation, and easy to implement

Active Publication Date: 2018-06-01
GUANGXI ACADEMY OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to promote the application of plant Lactobacillus, experts and scholars have done a lot of research on immobilization technology. Although the immobilization technology solves the shortcomings of large packaging containers and inconvenient transportation, the immobilization method is cumbersome, has high requirements for instruments and equipment, and has low cell viability. Weak and easily polluted, the product is expensive and the cost of use is high

Method used

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  • Method for extending shelf life of fishery liquid lactobacillus plantarum product
  • Method for extending shelf life of fishery liquid lactobacillus plantarum product
  • Method for extending shelf life of fishery liquid lactobacillus plantarum product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Preparation of Lactobacillus Plantarum Liquid for Fishery

[0040] Source of bacteria: obtained by enrichment, isolation and cultivation from the bottom mud of high-yield shrimp ponds by Guangxi Academy of Fishery Sciences, and identified as Lactobacillus plantarum.

[0041] Fermentation medium: molasses 30.0g, CH 3 COONa 6.0g, NaCl 6.0g, KH 2 PO 4 2.5g, K 2 HPO 4 2.0g, MgSO 4 ·7H 2 O 0.60g, yeast extract 1.5g, peptone 2.0g, CaCl 2 50.0mg, MnSO 4 25.0mg, FeSO 4 5.0mg, adjust the pH value to 6.0, aerate 1000ml of tap water.

[0042] Expanded cultivation: use 100L seed tanks for fermentation and cultivation, insert the prepared seed bacterial solution into the sterilized fermentation medium with an inoculum volume of 5% by volume, the filling coefficient is 90% (v / v), and stir evenly Afterwards, culture at a constant temperature and airtight at 40°C for 48 hours, and put them in tanks to end the culture.

[0043] According to the above process, 2 batches of ...

Embodiment 2

[0048] Effect of the initial pH value of the bacterial solution on the preservation effect of Lactobacillus plantarum in fish liquid

[0049] The batch number 1 Lactobacillus plantarum bacterial liquid prepared in Example 1 was adjusted to the initial pH value of the bacterial liquid with 2% dipotassium hydrogen phosphate buffer solution, and a total of 5 gradients were adjusted, which were 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5 each gradient set 2 repetitions, then divided into 2L polyethylene plastic bottles, a total of 14 bottles, placed in the room under natural conditions for 6 months and 12 months, after sampling to determine the bacterial concentration of the bacterial liquid And calculate the survival rate, the test results are shown in Table 2.

[0050] Table 2 The survival rate of Lactobacillus plantarum for fishery at different pH values ​​for 6 months and 12 months

[0051]

[0052]

[0053] From the measurement data in Table 2, it can be seen that Lactobacillus ...

Embodiment 3

[0055] Effect of Vitamin C on Preservation Effect of Lactobacillus Plantarum in Fishing Liquid

[0056] With the batch number 2 fishery Lactobacillus plantarum bacterium liquid prepared in Example 1, add the vitamin C of 0.0g / L, 0.25g / L, 0.5g / L, 0.75g / L and 1.0g / L in the bacterium liquid, adopt 2% dipotassium hydrogen phosphate buffer solution to adjust the pH value of the bacterial solution to 6.0, and then divide it into 2L polyethylene plastic bottles, set 2 repetitions for each gradient, a total of 12 bottles, and store them indoors under natural conditions for 6 months And after 12 months, take a sample to measure the thalline concentration of the bacterium liquid and calculate the survival rate, and the test results are shown in Table 3.

[0057] Table 3 The survival rate of Lactobacillus plantarum for fishery at different concentrations of vitamin C for 6 months and 12 months

[0058]

[0059] The results in Table 3 show that adding 0.25-1.0 g / L vitamin C to the fis...

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Abstract

Relating to the technical field of fishery microbial preparations, the invention provides a method for extending the shelf life of a fishery liquid lactobacillus plantarum product. The method includes: adding vitamin C into a fishery lactobacillus plantarum bacterial liquid to obtain a bacterial liquid containing 0.25-1.0g / L vitamin C; using a phosphate buffer solution to adjust the pH value of the obtained bacterial liquid to 4.5-7.0; sealing and packing the well adjusted lactobacillus plantarum, and conducting preservation at room temperature. The method provided by the invention has the advantages of simple operation, low cost, easy implementation and simple requirements for preservation conditions, can prolong the shelf life of the fishery liquid lactobacillus plantarum product and ensure the bacterial concentration, the survival rate of bacterial liquid cells preserved for 6 months can reach 89.36%, and the survival rate of bacterial liquid cells preserved for 12 months can reach80.85%, therefore the fishery liquid lactobacillus plantarum product preservation method provided by the invention is worthy of vigorous popularization and application.

Description

technical field [0001] The invention belongs to the technical field of microbial preparations for fishery, and in particular relates to a method for extending the shelf life of liquid plant lactobacillus products for fishery. Background technique [0002] Lactic acid bacteria (LAB) is a general term for bacteria that can produce large amounts of lactic acid from fermentable carbohydrates. These bacteria are widely distributed in nature and have rich species diversity. Due to the irrational use of antibiotics and hormone drugs in aquatic products, the problem of pesticide residues has posed a huge threat to human health. In the field of aquaculture, people are increasingly advocating ecological farming techniques. There are nearly 30 kinds of lactic acid bacteria among the more than 40 kinds of feed microorganisms stipulated by the FDA (1989) in the United States. The microbial varieties approved by the Ministry of Agriculture of my country in 1994 include Bacillus cereus,...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C12R1/25
CPCC12N1/04C12N1/20
Inventor 黎建斌李大列黄黎明杨学明吕敏陈福艳罗帮
Owner GUANGXI ACADEMY OF FISHERY SCI
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