Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for determining chitosan content by resonance Rayleigh scattering method sensitized by sodium dodecyl sulfate

A technology of sodium dodecyl sulfate and resonance Rayleigh scattering is applied in the field of macromolecular detection, which can solve the problems of complicated operation technology and enhanced resonance scattering intensity.

Inactive Publication Date: 2018-06-19
GUANGDONG PHARMA UNIV
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the technical deficiency that the content of chitosan in the finished product is difficult to quantify in the prior art, and operation technology is loaded down with trivial details, the present invention provides a kind of method of measuring chitosan content by the resonant Rayleigh scattering method of lauryl sodium sulfate sensitization Method, the present invention utilizes under certain conditions, chitosan can combine with amaranth to form an ion association, so that the resonance scattering intensity is significantly enhanced, adding sodium lauryl sulfate as a sensitizer to improve its sensitivity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining chitosan content by resonance Rayleigh scattering method sensitized by sodium dodecyl sulfate
  • Method for determining chitosan content by resonance Rayleigh scattering method sensitized by sodium dodecyl sulfate
  • Method for determining chitosan content by resonance Rayleigh scattering method sensitized by sodium dodecyl sulfate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0069] A method for measuring chitosan content by the resonance Rayleigh scattering method of sodium lauryl sulfate sensitization, it may further comprise the steps:

[0070] 1) Draw the standard curve of the chitosan concentration of ΔI and different molecular weights

[0071] Add 1.0mL chitosan standard solution with a certain concentration gradient, 1.0mL citric acid-sodium citrate buffer solution with pH=5.5, 1.0mL sodium dodecyl sulfate anion surface with a concentration of 0.086mol / L in the 10mL colorimetric tube Active agent solution, and 1.0 mL concentration of 1.0 x 10 -4 For the mol / L amaranth solution, use three times distilled water to make up to the mark, shake well, place in a water bath at 80°C for 10 minutes, cool to room temperature, take 1ml of the mixed solution and add it to a quartz cuvette, and put it in a F-2500 fluorescence spectrometer Photometer, with λ ex =λ em Carry out synchronous scanning, record respectively the resonance scattering intensity ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for determining chitosan content by a resonance Rayleigh scattering method sensitized by sodium dodecyl sulfate, and belongs to the field of macromolecule detection.Under certain conditions, chitosan can be combined with red amaranth to form an ionic associate to remarkably enhance resonance scattering intensity, and the sodium dodecyl sulfate is added as a sensitizer to improve the sensitivity. In a certain concentration range, the resonance Rayleigh scattering intensity and the chitosan content keep a linear relation in the certain concentration range, andthe resonance Rayleigh scattering intensity and the chitosan content keeping the linear relation are taken as a quantitative basis of the chitosan for establishment of the resonance Rayleigh scattering method of the chitosan. The method for determining the chitosan content is influenced by the molecular weight and the degree of deacetylation of the chitosan, and has the advantages of low cost, high sensitivity, good reproducibility and simple operation, and the like.

Description

technical field [0001] The invention relates to a method for measuring chitosan content through a resonance Rayleigh scattering method sensitized by sodium dodecyl sulfate, and belongs to the field of macromolecule detection. Background technique [0002] Chitosan (CTS), the chemical name is polyglucosamine (1,4)-2-amino-2-deoxy-D-glucan, is the main component of the exoskeleton of crustaceans (shrimp, crab) and insects , Chitosan is widely distributed in nature and belongs to the polymer green material. In recent years, as the research on chitosan in various countries in the world has gradually deepened, people's understanding and application of chitosan have been repositioned. Chitosan is natural, non-toxic, has good human affinity, can be used as pharmaceutical excipients, has excellent bactericidal properties and can be directly used as medicine and water treatment. In addition, chitosan also has important biomedical significance. It has the functions of reducing weigh...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/31G01N21/47G01N1/28G01N1/34
CPCG01N21/643G01N1/28G01N1/34G01N21/31G01N21/47G01N2021/4702
Inventor 白研苏政权
Owner GUANGDONG PHARMA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com