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Carboxyl functionalized polyurethane coated magnetic microsphere and preparation method thereof

A technology of carboxyl-functionalized magnetic microspheres, which is applied in the preparation of microspheres, microcapsule preparations, coatings, etc., can solve problems such as poor product monodispersity, uneven coating, and coating hydrophobicity that limit biomedical applications. Achieve strong biocompatibility, reduce the amount of protein, and reduce the effect of steric hindrance

Inactive Publication Date: 2018-06-22
SUZHOU ENRICHING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In CN201010145642.2, a superhydrophobic polyurethane / oxide nanoparticle hybrid coating material and its preparation method are proposed. Polyurethane is used as the wall material to prepare NdFeB powder microcapsules with a particle size of 1-5 μm, but due to its coating Hydrophobicity of layers limits applications in biomedicine
CN201510069423.3 discloses a preparation method of nano-silica polishing sheet by hydrolysis sol-gel method. Firstly, the surface of hydrophilic silica microspheres is coated with a layer of polyurethane coating, but it is a pity that there is no further Modification of functional groups makes it difficult to apply to the field of biomedicine
Patent CN201310352022.X has some changes in the synthesis method. First, polyurethane micelles are synthesized, and then the magnetic particles are coated. However, this method is likely to cause uneven coating and poor monodispersity of the product.

Method used

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  • Carboxyl functionalized polyurethane coated magnetic microsphere and preparation method thereof
  • Carboxyl functionalized polyurethane coated magnetic microsphere and preparation method thereof
  • Carboxyl functionalized polyurethane coated magnetic microsphere and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Preparation of monodisperse cross-linked polystyrene microspheres of 3 μm

[0043] Measure 320mL of absolute ethanol and 40mL of purified water, pour into a beaker and mix well. Accurately weigh 20g of PVP K30 and add it to the beaker, pour the above mixed solution into the flask, turn on the nitrogen gas to remove oxygen, and stir mechanically. Accurately weigh 1.0g of AIBN and add it to the beaker, then measure 72mL of styrene and pour it into the beaker, mix the two evenly and pour it into the flask, stir mechanically for 1 hour, then heat up to 50°C, pre-initiate for 30min, then heat up to 65°C mechanically After stirring for 4 hours, 20 mL of divinylbenzene and 50 mL of styrene were dissolved in 20 mL of ethanol and added dropwise to the flask, then the temperature was raised to 75° C., and the mixture was reacted for 16 hours. After the reaction, centrifuge and wash with ethanol and water three times respectively, and then dry and store the monocompone...

Embodiment 2

[0044] Example 2: Preparation of monodisperse cross-linked polystyrene microspheres of 0.8 μm

[0045] Measure 300mL of absolute ethanol and 20mL of purified water, pour into a beaker and mix well. Accurately weigh 12g of PVP K30 and add it to the beaker, pour the above mixed solution into the flask, turn on the nitrogen gas to remove oxygen, and stir mechanically. Accurately weigh 2g of AIBN and add it to the beaker, then measure 60mL of styrene and pour it into the beaker, mix the two evenly and pour it into the flask, stir mechanically for 1 hour, then heat up to 50°C, pre-initiate for 30min, then heat up to 65°C and stir mechanically After 4 hours, 40 mL of divinylbenzene and 58 mL of styrene were dissolved in 50 mL of ethanol and added dropwise to the flask, then the temperature was raised to 90° C., and the reaction was carried out for 20 hours. After the reaction, centrifuge and wash with ethanol and water three times respectively, and then dry and store the monocompon...

Embodiment 3

[0046] Example 3: Preparation of monodisperse cross-linked polystyrene microspheres of 5 μm

[0047] Measure 200mL of absolute ethanol and pour it into a beaker, accurately weigh 5g of PVP K30 and add it to the beaker, pour the above mixed solution into the beaker, turn on the nitrogen gas to remove oxygen, and stir mechanically. Accurately weigh 0.6g of AIBN and add it to the beaker, then measure 60mL of styrene and pour it into the beaker, mix the two evenly and pour it into the flask, stir mechanically for 1 hour, then heat up to 50°C, pre-initiate for 30min, then heat up to 65°C mechanically After stirring for 4 hours, 25 mL of divinylbenzene and 18 mL of styrene were dissolved in 45 mL of ethanol and added dropwise to the flask, then the temperature was raised to 85° C., and the reaction was carried out for 18 hours. After the reaction, centrifuge and wash with ethanol and water three times respectively, and then dry and store the monocomponent cross-linked polystyrene mi...

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Abstract

The invention relates to carboxyl functionalized polyurethane coated magnetic microspheres and a preparation method thereof. The preparation method comprises the following steps: firstly, preparing cross-linked polystyrene microspheres; secondly, performing surface functionization on the cross-linked polystyrene microspheres, and modifying one of amino, carboxyl, sulfonyl, methoxyl or chloromethyl; magnetizing the microspheres, and coating the magnetized cross-linked polystyrene microspheres by a polyurethane coating; finally performing carboxyl functionalization on the microspheres. The carboxyl functionalized polyurethane coated magnetic microspheres prepared by using the method have the characteristics of single dispersion, high suspension, high biocompatibility and relatively intense magnetic response, and have good practical values and application prospects in enzyme immobilization, immunoassay, cell separation, environment heavy metal detection analysis, and the like.

Description

technical field [0001] The invention relates to the field of nanometer and micron materials, in particular to a carboxyl-functionalized polyurethane-coated magnetic microsphere and a preparation method thereof. Background technique [0002] Magnetic microspheres have a wide range of applications in several medical and biochemical fields, for example, in patient treatment applications, application of a magnetic field can target drug-carrying magnetic microspheres to desired body sites. As another example, in diagnostic assay applications, magnetic separation techniques are utilized where a magnetic field is applied to a sample containing analytes bound to magnetic microspheres, allowing the analytes to be separated without the use of centrifugation or filtration. This technology uses magnetic particles as carriers, coated with biological molecules such as antigens, antibodies, enzymes, or modified functional groups. Separation of target molecules from samples such as biologi...

Claims

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Application Information

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IPC IPC(8): C08F212/08C08F212/36C08F257/02C08F220/06C08F222/14C08J7/14C08J7/12C08J7/04C08G18/32C08G18/76B01J13/14B01J13/22B01J13/20
CPCB01J13/14B01J13/20B01J13/22C08F212/08C08F257/02C08G18/3206C08G18/7671C08J7/0427C08J7/12C08J7/14C08J2325/06C08J2351/00C08J2475/08C08F212/36C08F216/1416C08F220/06
Inventor 李莹张佳斌曲峰尚春庆苏杰安新
Owner SUZHOU ENRICHING BIOTECH CO LTD
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