Method for synthesizing polyvinyl alcohol embolization microspheres capable of loading chemotherapeutic drug bleomycin
A technology of polyvinyl alcohol embolization microspheres and bleomycin, which is applied in the field of medical materials, can solve the problems of unreliable effects of cryotherapy and radiofrequency ablation, and many side effects. concise effect
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Embodiment 1
[0019] Add 150 g of polyvinyl alcohol into a flask filled with pure water, stir and disperse evenly. Heat up to 96°C, after the polyvinyl alcohol is completely dissolved, cool down to below 25°C, add 3.0g of intermediate acrylamidoalkyldialkoxy acetal, N-acrylamidodimethoxy Ethyl acetal 3.0g, after stirring for 10 minutes, add 100ml of concentrated hydrochloric acid dropwise to the solution, continue to stir for 6 hours after the dropwise addition, then collect the crude product, wash and dry to obtain the desired functionalized macromolecular hydrogel (referred to as Calli-B).
Embodiment 2
[0021] Add 12 g of sodium 2-acrylamide-2-methylpropanesulfonate and 10 g of potassium persulfate into water in sequence, dissolve and mix evenly, then add 100 g of the functionalized macromolecular gel intermediate (Calli-B) prepared in Example 1 and stir evenly to obtain a polymer monomer solution. Add 10 g of butyl acetate and 5 g of cellulose acetate into the reaction vessel, and feed N at the same time. 2 Gas, stirring, heating, and then sequentially add the above-mentioned polymer monomer solution and tetramethylethylenediamine to form an oil-water mixed reaction system, heating and stirring for 3 hours. In this reaction, the stirring paddle is an axial-flow stirring paddle, the stirring speed is controlled at 600 rpm, and the temperature of the reaction system is controlled at 60-70° C. when the polymer monomer solution is added. After the reaction is finished, the reaction mixture is filtered to collect the microspheres, washed with butyl acetate, ethyl acetate and ace...
Embodiment 3
[0023] Dissolve 10 mg of bleomycin in 2.0 ml of pure water, take the microsphere embolism synthesized in Example 2, accurately weigh 0.25 g, put it into a 20 ml vial, add 2 ml of the above-mentioned bleomycin solution, and make The microspheres were soaked in the bleomycin solution, and the mixture was shaken occasionally. Use a micro-syringe to regularly sample 20 μL at the 5th, 10th, 20th, 40th, and 60th minute, add 5.0ml of pure water to dilute, measure the absorbance at 485nm, and substitute the absorbance into the standard curve equation to calculate the concentration of the drug contained in the sample. The drug loading amount of the ball to bleomycin: drug loading=(drug content in the solution before drug loading-drug content in the solution after drug loading) / microsphere weight. The measured amount of bleomycin loaded on the microspheres was 25 mg / gram of microspheres.
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