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Culture method for improving fucoxanthin accumulation of phaeodactylum tricornutum bohlin

A technology of Phaeodactylum tricornutum fucoxanthin and a culturing method is applied in the cultivation field of improving the accumulation of Phaeodactylum tricornutum fucoxanthin, and can solve the problem that the accumulation of Phaeodactylum tricornutum fucoxanthin is not mentioned, and it is difficult to ensure the The absorption and utilization of Phaeodactylum algae, insoluble in water, etc., achieve the effect of facilitating large-scale operation, reducing the cost of aquaculture and processing, and avoiding multi-factor interference.

Active Publication Date: 2018-07-03
云南爱尔发生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Patent CN103898174 A discloses a culture method for improving the lipid accumulation of Phaeodactylum tricornutum. First, photoautotrophic culture is followed by dark stress, so as to provide an efficient, simple and pollution-free method for improving the lipid accumulation of Phaeodactylum tricornutum. culture method, but failed to mention the accumulation of fucoxanthin in Phaeodactylum tricornutum
[0008] Patents CN104531601 A, CN104531602 A, CN1045316013A, CN104531604 A, and CN105132352 A respectively disclose the use of arachidonic acid, ceric ammonium sulfate, acetylsalicylic acid, methyl jasmonic acid, and brassinosteroids to improve Phaeodactylum tricornutum fucoxanthin The method of element, but the substances used are insoluble in water, it is difficult to ensure the absorption and utilization of Phaeodactylum tricornutum

Method used

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  • Culture method for improving fucoxanthin accumulation of phaeodactylum tricornutum bohlin
  • Culture method for improving fucoxanthin accumulation of phaeodactylum tricornutum bohlin
  • Culture method for improving fucoxanthin accumulation of phaeodactylum tricornutum bohlin

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Experimental program
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Effect test

Embodiment 1

[0052] Take 4000L of Phaeodactylum tricornutum algae liquid for high-density culture in fully enclosed outdoors, OD 680 The value is 4.77, thickened by a high-speed disc centrifuge, and 1600L of dense algae liquid is collected, and the concentration ratio is 2.5:1. Prepared mine salt brine 400L, containing 27.7g / L sodium chloride, 5.20g / L magnesium chloride, 7.13g / L magnesium sulfate, 0.745g / L potassium chloride, 1.14g / L calcium chloride, and 0.837g sodium bromide / L, sodium bicarbonate 0.210g / L, add Pluvialls pluvialis exopolysaccharide 600g, equivalent to 1.5g / L, obtain heterotrophic culture fluid. Transfer the dense algae liquid and heterotrophic culture liquid into an inflatable mechanically stirred fermenter, stir, pass in a mixture of carbon dioxide and air, and cultivate for 100 hours at a temperature of 25°C to 28°C and a pH of 8.0 to 8.3, and harvest the triangles Phaeodactylum fresh algae, quick-frozen, refrigerated. At the same time, the samples submitted for insp...

Embodiment 2

[0054] Take 4000L of Phaeodactylum tricornutum algae liquid for high-density culture in fully enclosed outdoors, OD 680 The value is 4.12, thickened by a high-speed disc centrifuge, and 1600L of dense algae liquid is collected, and the concentration ratio is 2.5:1. Prepared mine salt brine 400L, containing 27.7g / L sodium chloride, 5.20g / L magnesium chloride, 7.13g / L magnesium sulfate, 0.745g / L potassium chloride, 1.14g / L calcium chloride, and 0.837g sodium bromide / L, sodium bicarbonate 0.210g / L, add Pluvialls pluvialis exopolysaccharide 600g, equivalent to 1.5g / L, obtain heterotrophic culture fluid. Transfer the dense algae liquid and heterotrophic culture liquid into an inflatable mechanically stirred fermenter, stir, pass in a mixture of carbon dioxide and air, and cultivate for 100 hours at a temperature of 25°C to 28°C and a pH of 8.0 to 8.3, and harvest the triangles Phaeodactylum fresh algae, quick-frozen, refrigerated. At the same time, the samples submitted for insp...

Embodiment 3

[0056] Take 4000L of Phaeodactylum tricornutum algae liquid for high-density culture in fully enclosed outdoors, OD 680 The value is 4.50, thickened by a high-speed disc centrifuge, and 1600L of dense algae liquid is collected, and the concentration ratio is 2.5:1. Prepared mine salt brine 400L, containing 27.7g / L sodium chloride, 5.20g / L magnesium chloride, 7.13g / L magnesium sulfate, 0.745g / L potassium chloride, 1.14g / L calcium chloride, and 0.837g sodium bromide / L, sodium bicarbonate 0.210g / L, add Pluvialls pluvialls exopolysaccharide 600g, equivalent to 2.0g / L, to obtain heterotrophic culture solution. Transfer the dense algae liquid and heterotrophic culture liquid into an inflatable mechanically stirred fermenter, stir, pass in a mixture of carbon dioxide and air, and cultivate for 100 hours at a temperature of 25°C to 28°C and a pH of 8.0 to 8.3, and harvest the triangles Phaeodactylum fresh algae, quick-frozen, refrigerated. At the same time, the samples submitted fo...

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Abstract

The invention discloses a culture method for improving fucoxanthin accumulation of phaeodactylum tricornutum bohlin. The culture method comprises the following steps: 1) culturing a dense phaeodactylum tricornutum bohlin solution; 2) preparing a culture solution from well and rock salt bittern and Haematococcus pluvialis Exopolysaccharides; and 3) carrying out heterotrophic culture on the concentrated algae solution and a heterotrophic culture solution in a fermentation tank to accumulate fucoxanthin. The accumulation of the fucoxanthin of the phaeodactylum tricornutum bohlin is improved. Thecontent of the fucoxanthin of the phaeodactylum tricornutum bohlin can be increased greatly, the breeding and processing cost can be reduced to a great extent, the economical benefit is improved and applications of the fucoxanthin are promoted.

Description

technical field [0001] The invention belongs to the technical field of microalgae cultivation, and in particular relates to a cultivation method for improving the accumulation of fucoxanthin in Phaeodactylum tricornutum. Background technique [0002] Fucoxanthin, also known as fucoidin, fucoxanthin, molecular formula C 42 h 58 o 6 , the structural formula is as figure 1 , molecular weight 658.91, density 1.09, melting point 166-168°C, CAS registration number 3351-86-8, belongs to allene-type carotenoids, and is an important carotenoid contained in brown algae, diatoms, golden algae and yellow-green algae Pigment is the brown substance of brown algae, and it is also an indicator pigment of diatom plants. [0003] Fucoxanthin has a unique structure and shows potential value advantages with promising application prospects in a variety of pharmacological activities, including anti-inflammatory, anti-tumor, anti-obesity, anti-oxidation, anti-acne, anti-diabetes, anti-malarial...

Claims

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Application Information

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IPC IPC(8): C12P17/02C12R1/89
CPCC12P17/02
Inventor 张吉和吴澄郑学军王兴勇张勇
Owner 云南爱尔发生物技术股份有限公司
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