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DNA immunoadsorbent, and preparation method thereof

An immunoadsorbent and pore adsorption technology, which is applied in the field of DNA immunoadsorbent and its preparation, can solve the problems of affecting the adsorption performance of the adsorbent, the decrease of DNA immobilization capacity, and insufficient DNA embedding, so as to improve the safety of use, The effect of reducing the amount of preflush and reducing particle shedding

Active Publication Date: 2018-07-06
JAFRON BIOMEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether it is activated carbon or carbonized resin, there is the problem that carbon particles are easy to fall off. Even if it is coated with collodion, it still cannot solve the problem of particle fall-off. During the treatment, the particles enter the human body with the blood circulation and cause serious adverse reactions such as thrombosis, allergies, and irritations; in addition, there are two disadvantages in the use of collodion embedding for DNA immobilization: (1) Some DNA will be damaged due to insufficient embedding. Sufficient, it may fall off in the subsequent production process, resulting in a decrease in the amount of DNA immobilized; (2) Part of the DNA that is embedded too deeply cannot contact the antibody; whether it is a decrease in the amount of DNA immobilized or embedded too deeply, it will affect the adsorption The adsorption performance of the agent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Preparation of polystyrene-based macroporous adsorption resin containing aldehyde groups:

[0043] In a 1000mL three-necked flask, add 500mL of aqueous solution containing 1.5wt% gelatin, add 7.5g of 4-vinylbenzaldehyde, 10.5g of ethyl styrene, 42g of divinylbenzene, 120g of cyclohexanol, and 0.6g of benzene peroxide For the mixed organic phase of formyl, under mechanical stirring, heat up to 75°C for polymerization reaction for 3 hours, heat up to 80°C for 7 hours, and then heat up to 85°C for 5 hours. Extract for 20 hours, wash with water until there is no smell of acetone, and filter with suction to obtain a polystyrene-based macroporous adsorption resin containing aldehyde groups, and screen out the resin with a particle size of 0.5-1.2 mm for future use.

[0044] The specific surface area, pore structure and other data were tested by nitrogen adsorption method, and it was known that the specific surface area of ​​the obtained resin was 760m 2 / g; the pore volu...

Embodiment 2

[0050] (1) Preparation of polystyrene-based macroporous adsorption resin containing aldehyde groups:

[0051] In a 1000mL three-necked flask, add 500mL of aqueous solution containing 1wt% polyvinyl alcohol, then add 15g of 3-vinylbenzaldehyde, 9g of ethyl styrene, 36g of divinylbenzene, 120g of toluene, and 0.6g of benzoyl peroxide. Mix the organic phase, heat up to 75°C for 3 hours under mechanical stirring, heat up to 80°C for 7 hours to solidify, then heat up to 85°C for 5 hours, after the reaction is over, evaporate the porogen toluene, wash with water until the washing solution is clear Finally, extract with acetone for 12 hours, wash with water until there is no smell of acetone, and filter with suction to obtain a polystyrene-based macroporous adsorption resin containing aldehyde groups. The resin with a particle size of 0.5-1.2 mm is selected for use.

[0052] The specific surface area, pore structure and other data were tested by nitrogen adsorption method, and it was...

Embodiment 3

[0058] (1) Preparation of polystyrene-based macroporous adsorption resin containing aldehyde groups:

[0059] Add 500mL of aqueous solution containing 0.1wt% carboxymethylcellulose to a 1000mL three-necked flask, add 20g 4-vinylbenzaldehyde, 8g ethylstyrene, 32g divinylbenzene, 90g toluene, 60g liquid paraffin, 0.6g For the mixed organic phase of benzoyl peroxide, under mechanical stirring, heat up to 75°C for polymerization reaction for 5 hours, heat up to 80°C for 6 hours, then heat up to 85°C for 5 hours, after the reaction, steam the porogen After soaking in toluene, wash with water until the washing solution is clear, extract with acetone for 12 hours, then wash with water until there is no acetone smell, and filter with suction to obtain a polystyrene-based macroporous adsorption resin containing aldehyde groups, with a particle size of 0.5-1.2mm The resin is spare.

[0060] The specific surface area, pore structure and other data were tested by nitrogen adsorption meth...

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PUM

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Abstract

The invention discloses a DNA immunoadsorbent and a preparation method thereof. The preparation method comprises the following steps: 1) preparation of polystyrene-based macroporous adsorption resin containing aldehyde groups; and 2) grafting and immobilization of DNA ligand. The DNA immunosorbent prepared by using the method directly adopts the macroporous adsorption resin as a carrier and replaces activated carbon or carbonized resin with the macroporous adsorption resin; and the toughness of a resin skeleton is retained and the risk of falling of particles is greatly reduced due to the omitting of carbonization and activation procedures during production. In addition, a chemical grafting method is employed to directly immobilize the DNA ligand to the macroporous adsorption resin, and the DNA ligand linked by a chemical bond is firmly immobilized and hardly falls off; moreover, without embedding and covering of coating materials like collodion, DNAs immobilized on the surface of theresin and inside the pores of the resin can exert maximum adsorption effect.

Description

technical field [0001] The invention relates to the field of blood purification, in particular to a DNA immunoadsorbent capable of adsorbing pathogenic substances in patients with systemic lupus erythematosus and a preparation method thereof. Background technique [0002] Systemic lupus erythematosus (SLE) is a relatively common autoimmune disease involving multiple systems and organs. Due to the tissue damage caused by a large number of pathogenic autoantibodies and immune complexes in the body, various system and organ damage may appear clinically, such as skin, joints, serosa, heart, kidney, central nervous system, blood system, etc. etc., the disease occurs all over the world, with a prevalence rate of 4 / 100,000 to 25 / 100,000, and a higher prevalence rate in Asia and black people. The prevalence rate in my country is 70 / 100,000 to 75 / 100,000 . The incidence of females is significantly more than that of males, about 10:1. Women of childbearing age are the peak incidence, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/30A61M1/36
CPCA61M1/3621A61M2210/12A61M1/362A61M1/3689B01J20/26
Inventor 董凡邵柯
Owner JAFRON BIOMEDICAL
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