A kind of microbial mixed flora and fermentation method for fermenting and producing lactic acid

A fermentation method and technology of mixed flora, applied in the field of bioengineering, can solve the problems of reduced production concentration and strength, reduced raw material utilization rate due to cell growth toxicity, and difficulty in utilization, etc., to achieve easy operation, reduced production labor costs, and durability Receptive effect

Active Publication Date: 2020-11-06
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the use of cheap raw materials can reduce the cost of raw materials to a certain extent, the impurities contained in them, such as volatile organic acids, colloids, ash, etc. contained in molasses, need to be purified before they can be used in pure culture biological processes
Without purification treatment, it is difficult to use a single bacterium, and its toxicity to cell growth reduces the utilization rate of raw materials, resulting in a decrease in final production concentration and strength

Method used

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  • A kind of microbial mixed flora and fermentation method for fermenting and producing lactic acid
  • A kind of microbial mixed flora and fermentation method for fermenting and producing lactic acid
  • A kind of microbial mixed flora and fermentation method for fermenting and producing lactic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Screening of domesticated lactic acid-producing microbial flora from bovine stomach contents

[0049] The samples were collected from a live animal slaughterhouse in Dalian. The samples of bovine stomach contents were processed in four ways: 1) suspension of bovine stomach contents, anaerobic enrichment; 2) suspension of bovine stomach contents, aerobic enrichment; 3) solid bovine stomach contents, anaerobic enrichment; 4) solid bovine stomach contents, aerobic enrichment. The preparation operation of the bovine stomach contents suspension: take 0.1kg of the fresh contents of bovine stomach in a 100mL triangular flask of physiological saline, filter the solid matter in it with sterile gauze, and take 5mL of the filtrate containing a large number of bacterial cells to inoculate the Erlenmeyer flasks and vials filled with 100mL enrichment medium for aerobic and anaerobic enrichment culture. Preparation operation of solid bovine stomach contents: take 0.1 kg of ...

Embodiment 2

[0053] Embodiment 2 The situation that microbial flora CEE-DL15 utilizes molasses under different operating conditions

[0054] The microbial flora CEE-DL15 obtained in Example 1 was seeded, inoculated into a vial containing 100 mL of MRS medium at a volume ratio of 5%, and fermented and cultured with sugarcane molasses as a substrate. The concentration is about 100g / L. Fermentation operation conditions are divided into three types, 1) sterilization fermentation, the substrate is sugarcane molasses after acid treatment; 2) sterilization fermentation, the substrate is untreated sugarcane molasses; 3) non-sterilization fermentation, the substrate is untreated Cane molasses. After 48 hours of shaking flask fermentation, measure the concentration, transformation rate and production intensity of lactic acid in the fermented liquid such as: figure 2 shown. The results showed that the bacterial group CEE-DL15 grew well without sterilization and using untreated molasses as the sub...

Embodiment 3

[0055] After the mixed bacterial group CEE-DL15 obtained in Example 1 was cultivated in the seed medium, it was inoculated into a vial containing 100 mL of fermentation medium at a volume ratio of 5% for cultivation, and the untreated sugarcane molasses was used as a substrate for fermentation and cultivation. , the initial untreated sugarcane molasses concentration was 250g / L. Fermentation medium is divided into three types: 1) MRS medium; 2) S-MRS medium; 3) CSLP medium; fermentation results such as image 3 shown. The bacterial colony can use the simple and cheap corn steep liquor dry powder medium (CSLP medium) to ferment lactic acid. The yield and conversion rate of lactic acid are similar to those of the other two mediums, and the production intensity is higher than that of the first two mediums. Save 50% to 200%.

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Abstract

The invention provides a microbial mixed flora for producing lactic acid by fermentation and a fermenting method. The microbial mixed flora comprises a clostridium bacterial strain, an escherichia bacterial strain and an enterococcus bacterial strain; the total effective viable count of the clostridium bacterial strain, the escherichia bacterial strain and the enterococcus bacterial strain occupies 94 percent or above of effective viable count in the microbial mixed flora; the ratio of the effective viable counts of the clostridium bacterial strain to the escherichia bacterial strain to the enterococcus bacterial strain is (40-60):(20-40):(2-20). Relative to single bacteria fermentation, the microbial mixed flora is good in fermentation stability, high in biological safety and high in molasses tolerance, can also grow and metabolize in untreated molasses of 500g / L and has high production strength and lactic acid conversion rate. The microbial mixed flora also has the advantages of no sterilization in the production process, no fermentation substrate molasses pretreatment, low production cost and the like.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and particularly relates to a method for producing lactic acid by using molasses as a substrate by fermenting mixed bacteria groups of microorganisms. Background technique [0002] Lactic acid is an important biochemical product, widely used in medicine, food, beverage, chemical industry, leather, cigarette industry and other fields. The most promising application of lactic acid is in the field of biomaterials represented by polylactic acid (PLA). As the most potential biodegradable polymer material, it is expected to replace non-degradable plastics such as polypropylene, polyethylene, and polystyrene, and eliminate "white pollution". It is a bio-environmental material with great application potential. [0003] The production methods of lactic acid include chemical synthesis method, enzymatic method and microbial transformation method. Compared with chemical method and enzymatic method, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/56C12R1/145C12R1/185C12R1/01
CPCC12N1/20C12P7/56
Inventor 孙亚琴徐振振周瑾洁修志龙
Owner DALIAN UNIV OF TECH
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