Preparation of microelectrochemical sensor for detection of dopamine concentration in animal brain

A technology for concentration detection and dopamine is applied in the field of electrochemical analysis and detection of neurotransmitter dopamine, and achieves the effects of high sensitivity, small volume and simple preparation steps

Inactive Publication Date: 2018-07-06
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, according to the data search conducted by the applicant, in the existing electrochemical analysis and detection technology or patent literature of dopamine, there is no preparation of silicon-based microelectrode arrays modified by porous gold platinum nanoparticles and perfluorosulfonic acid (Nafion) membranes. A report on a miniature electrochemical sensor that can be used to detect the concentration of dopamine in the animal brain

Method used

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  • Preparation of microelectrochemical sensor for detection of dopamine concentration in animal brain
  • Preparation of microelectrochemical sensor for detection of dopamine concentration in animal brain
  • Preparation of microelectrochemical sensor for detection of dopamine concentration in animal brain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Preparation of a Micro Electrochemical Sensor for Detection of Dopamine Concentration in Animal Brains

[0023] Step 1: The schematic diagram of the structure of the silicon-based microelectrode array used in this example is shown in figure 1 As shown, including the pad area and the electrode site area of ​​different sizes, each electrode in the electrode site area of ​​different sizes is connected to each connection point on the pad area by electrode leads, and the total length of the silicon-based microelectrode array is 15mm ( That is, the total length from the front tip of the electrode site area of ​​different sizes to the distal edge of the pad area is 15 mm).

[0024] The silicon-based microelectrode array is 300μm-500μm wide, 100-200μm thick, and 8-9mm long. The electrode sites on the surface of the silicon-based microelectrode array are circular gold sites with a diameter of 50 μm, and the circular gold sites are used as electrodes to be modified lo...

Embodiment 2

[0026] Example 2: Working curve drawing for dopamine miniature electrochemical sensor in animal brain

[0027] Step 1: use the dopamine concentration detection miniature electrochemical sensor prepared in Example 1 as the working electrode, the platinum wire electrode as the counter electrode, and the Ag / AgCl electrode as the reference electrode, and put it into an electrolytic cell containing 10 mL of pH=7.4 phosphate buffer In the three-electrode system, and connected to the electrochemical workstation;

[0028] Step 2: Using constant voltage current voltammetry, apply a constant voltage of +0.3V between the working electrode and the reference electrode, and then dropwise add 0.05μM, 0.1μM, 0.2μM, 0.7μM in the case of uniform stirring of the electrolytic cell , 1 μM, 2 μM, 2 μM, 2 μM, 2 μM, 2 μM dopamine standard solution, obtained as figure 2 The dopamine oxidation current step curve is shown.

[0029] According to the relationship between the obtained dopamine oxidation...

Embodiment 3

[0032] Embodiment 3: Anti-disturbance test of miniature electrochemical sensor for dopamine concentration detection in animal brain

[0033] Step 1: The micro-electrochemical sensor for detecting dopamine concentration in the animal brain prepared in Example 1 was used as the working electrode, the platinum wire electrode was used as the counter electrode, and the Ag / AgCl electrode was used as the reference electrode, and 10 mL of pH=7.4 phosphoric acid In the electrolytic cell of the buffer solution, a three-electrode system is formed and connected to the electrochemical workstation;

[0034] Step 2: Using constant voltage current voltammetry, apply a constant voltage of +0.3V between the working electrode and the reference electrode, and then add 1 μM dopamine, 12 μM dihydroxyphenylacetic acid, and 12 μM ascorbic acid dropwise in the case of uniform stirring in the electrolytic cell , 0.5 μM dopamine, 1 μM dopamine solution, wherein dihydroxyphenylacetic acid and ascorbic ac...

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Abstract

The invention discloses a preparation method of a microelectrochemical sensor for detection of dopamine concentration in an animal brain, wherein a silicon-based microelectrode array is used as an electrode to be modified; firstly, an electrodeposition method and a chemical etching method are used to deposit a layer of porous gold-platinum nanoparticles on the electrode sites of the silicon-basedmicroelectrode array, then an electropolymerization is used to deposit a layer of perfluorosulfonic acid (Nafion) film on the electrode sites modified with the gold-platinum nanoparticles to obtain the microelectrochemical sensor for detection of dopamine concentration. The prepared microelectrochemical sensor for detection of dopamine concentration in the animal brain is implanted into striatum nuclei of the animal brain, and is connected as a working electrode to an electrochemical workstation to realize detection of dopamine concentration in the animal brain by detecting of a dopamine electrochemical signal, and the microelectrochemical sensor is simple in preparation, high in sensitivity, strong in selectivity, and small in volume.

Description

technical field [0001] The invention belongs to the technical field of electrochemical analysis and detection of neurotransmitter dopamine, in particular to a preparation method of a micro-electrochemical sensor for detecting dopamine concentration in animal brains. Background technique [0002] As an important neurotransmitter in mammals, dopamine plays an important role in the nervous system, cardiovascular system and immune system; in addition, dopamine, as a catecholamine neurotransmitter, is closely related to the emotion, spirit and body of animals. Exercise, regulation of muscle tension, and endocrine activity are closely linked. Abnormal changes in the concentration of dopamine in the nervous system may lead to neurological diseases such as Parkinson's disease, schizophrenia, depression and epilepsy. Therefore, accurate detection of dopamine concentration in the nervous system is of great significance for the study of the pathogenesis and prevention and treatment of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 赵宗亚于毅袁庆丽李中伟任武王昌
Owner XINXIANG MEDICAL UNIV
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