A method for rapid screening of high-yield lovastatin Monascus

Abstract

The invention discloses a method for quickly screening high-yield lovastatin monascus, using micro-plate high-throughput culture technology (solid and liquid) to cultivate monascus, and at the same time using a dual-wavelength method (△A=A 238 -A 247 ) Rapid detection of lovastatin content in fermentation broth. Compared with conventional plate culture and shake flask fermentation, the microporous plate high-throughput culture technology described in the present invention has a small sample size, many types of samples that can be processed at one time, saves space, and has a high utilization rate of the incubator; for high-yield For the rapid screening of lovastatin strains, compared with liquid chromatography, the experiment period of the present invention is greatly shortened; compared with the colorimetric method (single wavelength or dual wavelength) reported in the literature, the present invention can more eliminate interference from other substances. In a word, the whole process can quickly cultivate and screen hundreds of Monascus strains at the same time. The whole process has a small workload, fast and convenient operation, and is easy to implement.

Description

technical field [0001] The invention belongs to the technical field of monascus screening, in particular to a method for rapidly screening high-yield lovastatin monascus. Background technique [0002] Monascus is a yellow wine brewing fungus isolated and purified from red yeast rice. Monascus can produce many beneficial metabolites. Lovastatin is one of them, which has the effects of inhibiting cholesterol synthesis and lowering blood lipids. The purpose of screening Monascus with high yield of lovastatin is mainly to prepare functional Monascus powder, so that the pigment of Monascus is rich in lovastatin with a higher content. [0003] In laboratory research, the screening of Monascus generally requires plate activation and shake flask fermentation. With the recent rise of micro-plate fermentation technology, micro-plate gradually replaces shake flask fermentation as a high-throughput culture and screening technology, but the early expansion of Monascus still stays on the...

AI Technical Summary

Problems solved by technology

With the recent rise of micro-plate fermentation technology, micro-plate gradually replaces shake flask fermentation as a high-throughput culture and screening technology, but the early expansion of Monascus still stays on the old technology of plate activation

On the other hand, after the high-throughput culture technology, a new rapid detection technology has yet to be established. Most of the rapid screening methods for lovastatin-producing bacteria in the literature are based on liquid chromatography detection technology. Although liquid chromatography is accurate, it is relatively expensive. time, and with the increase of the detection time, the samples to be tested in the back will inevitably be denatured; some scholars use the colorimetric method to quickly screen lovastatin, but the single-wavelength method (A 238 ) interference is too strong, it is difficult to explain the substance at this wavelength is lovastatin, and some scholars have invented a dual-wavelength method (△A=A 246 -A 254 ) for rapid detection of lovastatin, but this method only excludes the interference of red yeast rice pigment and does not eliminate the interference of other substances in the fermentation broth. In fact, in the red yeast fermentation broth without lovastatin, △A= A 246 -A 254 It is difficult to approach 0, so that the use of this method is limited

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