Aureomycin fermentation culture medium capable of reducing aureomycin loss in filtrate

Abstract

The invention relates to an aureomycin fermentation culture medium capable of reducing aureomycin loss in filtrate. Each liter of the aureomycin fermentation culture medium aqueous solution contains 20 to 25g of peanut cake powder, 6 to 15g of soybean cake powder, 100 to 120g of corn starch, 1 to 5g of yeast powder, 0.1 to 0.5g of amylase, 2 to 3g of sodium chloride, 5 to 8g of ammonium sulfate, 4to 6g of calcium carbonate, 2 to 4g of calcium lactate, 0.1 to 0.3g of magnesium sulfate, and 0.1 to 0.2g of soya-bean oil. According to the aureomycin fermentation culture medium, calcium lactate isadopted to replace a part of calcium carbonate in an original formula, so that effective chelating of free aureomycin secreted by bacterial strains in fermentation process is realized, inhibition ofextracellular aureomycin on bacterial strain growth is relieved, and bacterial strain aureomycin production capacity is improved; and at the same time, a calcium aureomycin insoluble substance is generated from calcium lactate and extracellular free aureomycin, so that loss of aureomycin in subsequent frame and plate filtration is reduced, on one hand aureomycin recovery rate is increased, and onthe other hand aureomycin residue in filtrate is reduced.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a chlortetracycline fermentation medium for reducing the loss of chlortetracycline in filtrate. Background technique [0002] The aureomycin premix is ​​mainly prepared by fermenting and cultivating Streptomyces aureus, adding calcium carbonate to the fermented liquid for mixing after the fermentation, plate and frame filtration to obtain a wet filter cake, drying and pulverizing the wet filter cake. [0003] Aureomycin is mainly an intracellular product of Streptomyces aureus. However, during the culture process, aureomycin strains will secrete a part of aureomycin products extracellularly. Under normal circumstances, aureomycin strains have a certain range of tolerance to extracellular aureomycin. Aureomycin will inhibit the growth of the strain and the synthesis of aureomycin. Therefore, the extracellularly secreted aureomycin, on the one hand, wil...

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Problems solved by technology

Zhumadian Huazhong Chia Tai Co., Ltd. Li Shuzhi et al.’s patent on chlortetracycline fermentation medium and the production method of aureomycin fermentation using the medium (201310504063.6); Jinhe Biotechnology Co., Ltd. Xie Changxian et al. Patent for breeding medium and strain selection method (201510485721.0); Pucheng Zhengda Biochemical Co., Ltd.’s patent for a method of aureomycin fermentation using refined protein powder (201610694105.0); Ningxia Tairui Pharmaceutical Co., Ltd. Ren Yong et al. on a new culture medium and culture method for aureomycin fermented by Streptomyces aureus (201410057535.2), Zhumadian Huazhong Chia Tai Co., Ltd. Li Dapan et al. Patent (201611183084.2) for the application in Hebei Shengxue Dacheng Pharmaceutical Co., Ltd. Zhao Lihong and others on a chlortetracycline fermentation medium (201510687867.3); Zhumadian Huazhong Chia Tai Co., Ltd. Liu Juan and others on a chlortetracycline The patent of the fermentation medium and the fermentation production method of aureomycin using the medium (201611199651.3); the patent of Jinhe Biotechnology Co., Ltd. Xie Changxian and others on the decomposition and application of the aureomycin fermentation medium (201510295435.8); these patents Both described containing the only calcium salt - calcium carbonate CaCO 3 None of these patents involves the research and application of another calcium salt, calcium lactate

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