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Application of red anise glycoside ether and composition in treatment of gouty arthritis

A technology of gouty arthritis and red fennel, applied in the field of medicine, can solve problems such as unreported

Pending Publication Date: 2018-07-20
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the application of the red anisoside of the present invention and the composition in gouty arthritis.

Method used

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  • Application of red anise glycoside ether and composition in treatment of gouty arthritis
  • Application of red anise glycoside ether and composition in treatment of gouty arthritis
  • Application of red anise glycoside ether and composition in treatment of gouty arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] The preparation of embodiment 1 red anisole

[0069] 1 Instruments and reagents

[0070] Infrared (IR) spectrometer: Nicolet 5700FT-IR spectrometer

[0071] Ultraviolet (UV) spectrometer: Shimadzu UV-210A spectrophotometer

[0072] Nuclear magnetic resonance (NMR) instrument: Bruker AC-300or DRX-600spectrometers with TMS as aninternal standard

[0073] Mass spectrometer (MS) instrument: API QSTAR Pulsar 1spectrometer (HR-ESI-MS)

[0074] Semipreparative HPLC instrument: Agilent 1100liquid chromatograph with aZorbax SB-C 18 ,9.4mm×25cm

[0075] Ultrasonic cleaner: KQ-501E type, Kunshan Ultrasonic Instrument Co., Ltd.

[0076] Column chromatography packing: column chromatography silica gel H (reagent grade, 100-200 mesh, 200-300 mesh), Shandong Qingdao Ocean Chemical Factory; Sephadex LH-20, GE Healthcare Bio-Science AB (Switzerland)

[0077] Thin layer chromatography silica gel plate: HSGF 254 (20×20cm, 5×20cm), Shandong Yantai Zhifu Huangwu Silicone Development a...

Embodiment 2

[0092] The preparation of embodiment 2 total glycosides of red anise

[0093] 30kg of red fennel medicinal material, pulverized, heated and refluxed with 80% ethanol for extraction, the extract was filtered and concentrated under reduced pressure to form an extract (1.52kg), the extract was evenly dispersed in water, and extracted with equal volumes of petroleum ether and ethyl acetate , the combined ethyl acetate parts were concentrated under reduced pressure to obtain extract (251.6g), passed through polyamide column and AB-8 macroporous resin column respectively, and the combined fractions were concentrated to obtain 32.5g of total glycosides TG-IL, which was determined by HPLC , of which quercetin contains 32.1%, maplein 5.6%, anisole 0.41%.

Embodiment 3

[0094] Embodiment 3 red anisoside anti-inflammatory activity

[0095] Effects of anisole and TG-IL on the degree of ear swelling in mice induced by xylene

[0096] 1 Animals, Reagents and Materials

[0097] Experimental animals: ICR male mice (18-22g) were purchased from Shanghai Slack Experimental Animal Co., Ltd.; the mice were kept at room temperature and had free access to food and water; they were fasted for 12 hours before the experiment.

[0098] Positive control drug: Dexamethasone acetate tablets (Dexamethasone), Shanghai Pharmaceutical (Group) Co., Ltd. Xinyi Pharmaceutical General Factory

[0099] Inflammatory agent: Xylene, analytically pure, Sinopharm Chemical Reagent Co., Ltd., batch number F20100303

[0100] The dosages of anisole ZM-IL are both 20mg / kg and 80mg / kg, and the dosages of total anishole glycosides TG-IL and quercetin Q-IL are both 100mg / kg and 400mg / kg, before use Prepare 2mg / ml and 10mg / ml suspensions with 0.5% CMC-Na aqueous solution, and shake...

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Abstract

The invention relates to a red anise fat ether, a red anise glycoside ether, red anise total glycosides, and a preparation method and an application thereof in the treatment of gouty arthritis. A redanise novel lignin component 5,5'-diallyl-2,2',3'-triethoxyl diphenyl ether (red anise fat ether) is obtained, and people find that red anise fat ether has anti-inflammation activity. Red anise totalglycosides are obtained, comprise red anise fat ether, difengpin, and quercitrin, have anti-inflammation activity, and have a curative effect on treating gouty arthritis, and the curative effect is testified by animal tests.

Description

technical field [0001] The invention relates to the technical field of medicines, in particular to the application of red anisoside and its composition in gouty arthritis. Background technique [0002] I.lanceolatum, a plant of the genus Anise in the family Aniseaceae, also known as fennel lanceolatum, red poisonous fennel, and mangcao, is a unique plant in my country. It is distributed in southern China. Its root or root bark is used as medicine, and the fruit is highly poisonous. Red fennel has the effects of dispelling wind and dredging collaterals, dissipating blood stasis and relieving pain; it can be used for bruises, rheumatic arthralgia, carbuncle, swelling and poison, and intestinal parasitic diseases. The clinically used Chinese patent medicine red fennel injection is also extracted from the root bark of lanceolatum (I.lanceolatum). It has the effects of reducing swelling, dissipating blood stasis, promoting blood circulation and relieving pain. It is used for lumb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/09C07C41/34C07C43/215A61K36/57A61K31/7048A61K31/235A61P29/00A61P19/02A61P1/00A61P17/06A61P11/06
CPCA61K31/09A61K31/235A61K31/7048A61K36/57C07C41/34A61K2236/39A61K2236/53A61K2236/51A61K2236/55A61K2236/333C07C43/215A61K2300/00
Inventor 黄宝康杨德俊薛兢兢韩婷郑承剑蒋益萍贾敏
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY