Unlock instant, AI-driven research and patent intelligence for your innovation.

Lycopene-loaded self-assembled nanomicelles and preparation method thereof

A technology of lycopene and nano micelles, which is applied in the directions of drug combinations, pharmaceutical formulations, medical preparations of inactive ingredients, etc., can solve the problems such as nano micelles that have not yet been seen, and achieves mild preparation conditions and uniform particle size distribution. , good stability

Active Publication Date: 2020-04-28
XINJIANG AGRI UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the research on polymer drug-loaded micelles involves multiple disciplines such as polymers, pharmaceuticals, pharmacology, pathology, and molecular biology, there have been no reports on self-assembled nanomicelles loaded with lycopene at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lycopene-loaded self-assembled nanomicelles and preparation method thereof
  • Lycopene-loaded self-assembled nanomicelles and preparation method thereof
  • Lycopene-loaded self-assembled nanomicelles and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Weigh polyethylene glycol monomethyl ether-polylactic acid block copolymer (mPEG-PLA, the mass ratio of polyethylene glycol 5000 monomethyl ether to polylactic acid is 45:55) 30mg, lycopene 1.5mg, lecithin 5mg dissolved in 2mL dichloromethane, added 6mL phosphate buffer (pH7.4), emulsified with a high-speed shearing machine at 13000rpm for 1min to form an oil-in-water emulsion, transferred the emulsion to a rotary evaporator, and evaporated at 30℃ The organic solvent was removed, and the unencapsulated free lycopene was filtered through a 0.2μm microporous membrane to prepare a lycopene self-assembled nanomicelle preparation with a micelle particle size of 113nm and a particle size distribution (PDI) of 0.220, the encapsulation rate is 80%.

Embodiment 2

[0046] Weigh 40 mg of mPEG-PLA (the mass ratio of polyethylene glycol 5000 monomethyl ether to polylactic acid is 30:70), 2 mg of lycopene and 5 mg of Tween 80, dissolve in 2 mL of chloroform, mix with 8 mL of normal saline, and use high speed Emulsify at 13000rpm for 1min with a shearing machine to form an oil-in-water emulsion. Transfer the emulsion to a rotary evaporator. Rotary evaporation at 35°C to remove all organic solvents. Filter through a 0.2μm microporous membrane to remove unencapsulated free lycopene. , The lycopene self-assembled nanomicelle preparation was prepared, the micelle particle size was 130 nm, the particle size distribution (PDI) was 0.246, and the encapsulation efficiency was 75%.

Embodiment 3

[0048] Weigh 40 mg of mPEG-PLA (the mass ratio of polyethylene glycol 5000 monomethyl ether to polylactic acid is 40:60), 3 mg of lycopene and 10 mg of Span 80, dissolved in 10 mL of dichloromethane, and mixed with 2 mL of distilled water. Emulsify for 1 min with a high-speed shearing machine at 13000 rpm to form a water-in-oil emulsion. Transfer the emulsion to a rotary evaporator. Rotary evaporation at 30°C to remove all organic solvents. Filter through a 0.2μm microporous membrane to remove unencapsulated free tomato red. The lycopene self-assembled nano-micelle preparation was prepared, the micelle particle size was 120nm, the particle size distribution (PDI) was 0.231, and the encapsulation efficiency was 67%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a self-assembled nano micelle preparation with entrapped fat soluble lycopene. The preparation is composed of following components in percentage by weight: 1 to 40% of lycopene, 60 to 99% of methoxypolyethylene glycol-polylactic acid segmented copolymer (mPEG-PLA), and 1 to 20% of emulsifier. The obtained lycopene self-assembled nano micelle has the advantages of uniform size distribution, high encapsulation rate, and good biocompatibility. The stability of lycopene is improved. The water solubility of lycopene is improved. The bioavailability of lycopene is increased.The invention also discloses a preparation method of the self-assembled nano micelle preparation with entrapped fat soluble lycopene. The preparation technology is simple and controllable. According to the preparation method, lycopene, mPEG-PLA, and an emulsifier are dissolved by an organic solvent, the solution is mixed with a water solution, then the solution is emulsified by a high speed shearing machine, the emulsion is transferred to a rotary evaporator, rotary evaporation is carried out at a temperature of 30 to 35 DEG C to remove the organic solvent, and then the emulsion is filtered bya microporous filter membrane (0.2 [mu]m) to remove non-encapsulated free lycopene to obtain the lycopene loaded self-assembled nano micelle.

Description

Technical field [0001] The invention relates to a micelle preparation for carrying hydrophobic drugs and the preparation field thereof, in particular to a self-assembled nano micelle preparation for carrying lycopene and a preparation method thereof. Background technique [0002] Lycopene is a fat-soluble acyclic polyunsaturated olefin, molecular formula C 40 H 56 , The relative molecular mass is 536.85. It is estimated that there are about 72 lycopene isomers. Naturally sourced lycopene mainly exists in the most stable all-trans structure ( figure 1 ). Lycopene is insoluble in water, hardly soluble in polar organic solvents such as methanol, soluble in ether, petroleum ether, hexane, acetone, and easily soluble in chloroform, dichloromethane, carbon disulfide, benzene, oil, etc., lycopene samples The more pure, the more difficult it is to dissolve. Humans cannot synthesize lycopene by themselves, and can only be supplemented by diet. After absorption, lycopene can be widely dis...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/107A61K47/34A61K31/01A61P39/06A61P9/00A61P9/10A61P35/00A61P17/00C08G63/08C08G65/332
CPCA61K9/1075A61K31/01A61K47/34C08G63/08C08G65/3322
Inventor 朱金芳颜安包晓玮
Owner XINJIANG AGRI UNIV