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Photoluminescent citric acid based nanoparticles and application and method for living cell marking

A technology of photoluminescence and citric acid, applied in the direction of luminescent materials, fluorescence/phosphorescence, chemical instruments and methods, etc., can solve the problems of weak fluorescence signal intensity, limit the application of quantum dots, poor photostability, etc., and achieve good biocompatibility properties, excellent photoluminescent properties, and good photostability

Active Publication Date: 2018-07-27
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Inorganic quantum dots have excellent photoluminescence and photostability, and can stably label cells for several months, but their potential cytotoxicity due to their heavy metal elements and non-degradable properties greatly limits the use of quantum dots in living cell labeling. Further applications in
Organic dye cell markers have poor photostability, weak fluorescence signal intensity and certain toxicity, so their application in cell labeling is limited

Method used

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  • Photoluminescent citric acid based nanoparticles and application and method for living cell marking
  • Photoluminescent citric acid based nanoparticles and application and method for living cell marking
  • Photoluminescent citric acid based nanoparticles and application and method for living cell marking

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1 Preparation of photoluminescent citric acid-based nanoparticles

[0032] (1) Preparation of poly(citric acid-siloxane) polymer material: Add 1,8-octanediol, citric acid, and 3-aminopropyltriethoxysilane in a molar ratio of 1:1:0.4 to a 50mL flask and placed in an oil bath, the flask was heated to 160°C with stirring ° C, to be dissolved and then at 140 ° C for one hour to obtain a polymer, the whole process is carried out under the protection of nitrogen, the polymer is washed three times with deionized water, and it is freeze-dried for 48 hours to obtain a poly(citric acid-siloxane) polymer material ;

[0033] (2) Add 200 mg of the poly(citric acid-siloxane) polymer material obtained in step (1) into 10 mL of dimethyl sulfoxide, and stir with a magnetic stirrer for 30 minutes at room temperature to dissolve;

[0034] (3) Add the poly(citric acid-siloxane) solution in step (2) dropwise into 50mL of 1% polyvinyl alcohol solution at a stirring speed of 150 rp...

Embodiment 2

[0036] Example 2 Preparation of photoluminescent citric acid-based nanoparticles

[0037] (1) Preparation of poly(citric acid-siloxane) polymer material: Add 1,8-octanediol, citric acid, and 3-aminopropyltriethoxysilane in a molar ratio of 1.1:1:0.5 to a 50mL flask and placed in an oil bath, the flask was heated to 160°C with stirring ° C, to be dissolved and then at 140 ° C for one hour to obtain a polymer, the whole process is carried out under the protection of nitrogen, the polymer is washed three times with deionized water, and it is freeze-dried for 48 hours to obtain a poly(citric acid-siloxane) polymer material ;

[0038](2) Add 100 mg of the poly(citric acid-siloxane) polymer material obtained in step (1) into 10 mL of dimethyl sulfoxide, and stir with a magnetic stirrer for 30 minutes at room temperature to dissolve;

[0039] (3) Add the poly(citric acid-siloxane) solution in step (2) dropwise into 50mL of 1.5% polyvinyl alcohol solution with a stirring speed of 1...

Embodiment 3

[0041] Example 3 Preparation of photoluminescent citric acid-based nanoparticles

[0042] (1) Preparation of poly(citric acid-siloxane) polymer material: Add 1,8-octanediol, citric acid, and 3-aminopropyltriethoxysilane in a molar ratio of 0.9:1:0.2 to a 50mL flask and placed in an oil bath, the flask was heated to 160°C with stirring ° C, after dissolving at 140 ° C for one hour to obtain a polymer, the whole process is carried out under the protection of nitrogen, the polymer is washed three times with deionized water, and it is freeze-dried for 48 hours to obtain a poly(citric acid-siloxane) polymer material ;

[0043] (2) Add 300 mg of the poly(citric acid-siloxane) polymer material obtained in step (1) into 10 mL of dimethyl sulfoxide, and stir with a magnetic stirrer for 30 minutes at room temperature to dissolve;

[0044] (3) Add the poly(citric acid-siloxane) solution in step (2) dropwise into 50mL 0.5% polyvinyl alcohol solution, stirring at 150 rpm;

[0045] (4) ...

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Abstract

The invention relates to photoluminescent citric acid based nanoparticles and an application and a method for living cell marking. The photoluminescent citric acid based nanoparticles have the advantages as follows: (1) the nanoparticles have excellent photoluminescent performance, can excite fluorescence imaging at the wave band of 360-480 nm and have the fluorescence quantum yield up to 15%; (2)the nanoparticles have good light stability and have the fluorescence emission spectrum not changed obviously and have high brightness after being soaked in a phosphate buffer solution and a completecell culture medium (DMED+10% fetal calf serum); (3) the nanoparticles have good biocompatibility and facilitate adhesion and proliferation of cells; (4) the photoluminescent citric acid based nanoparticles can mark stem cells and tumor cells in real time for a long time and have the marking time in vitro as long as 14 days, which is far longer than that of commercial cell markers; (5) the preparation process is simple and industrial production can be realized.

Description

technical field [0001] The invention relates to a photoluminescence citrate-based nanoparticle, its application and method for living cell labeling. Background technique [0002] Stem cell and tumor cell labeling and imaging are of great significance in the study of stem cell transplantation and tumor cell development. Currently commonly used cell labeling materials mainly include inorganic quantum dot materials and organic dyes such as fluorescein isothiocyanate, rhodamine isothiocyanate, and indole cyanine dye Cy3. Inorganic quantum dots have excellent photoluminescence and photostability, and can stably label cells for several months, but their potential cytotoxicity due to their heavy metal elements and non-degradable properties greatly limits the use of quantum dots in living cell labeling. further applications in. Organic dye cell markers have poor photostability, weak fluorescence signal intensity and certain toxicity, so their application in cell labeling is limite...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/569G01N33/50G01N33/533G01N33/574C09K11/06
CPCC09K11/06C09K2211/14G01N21/64G01N21/6402G01N21/6428G01N21/6458G01N33/5005G01N33/533G01N33/56966G01N33/574G01N2021/6417G01N2021/6439G01N2021/6495
Inventor 毛葱雷波金宛宛王成贵
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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