Broad bean natto and preparation method thereof
A technology of natto and broad bean, which is applied in the field of broad bean natto and its preparation, can solve the problems of reduced enzyme activity and viable bacteria count, low consumer acceptance, weakening the advantages of nattokinase, etc., and achieves high enzyme activity and improved stability Sexuality, shelf life, and low cost
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[0047] The present invention also provides a kind of preparation method of broad bean natto, comprises the following steps:
[0048] Raw material screening: remove impurities and bad beans in broad beans and soybeans respectively;
[0049] Soaking: Wash the broad beans, add water 3 to 4 times the weight of the broad beans, and soak until there are no wrinkles on the surface; wash the soybeans, add water 3 to 4 times the weight of the soybeans, and soak at room temperature for 10 to 24 hours;
[0050] Cooking of raw materials: Broad beans and soybeans are cooked with high-pressure steam;
[0051] Mixing: mix the steamed broad beans and soybeans according to the formula while they are hot;
[0052] Inoculation fermentation: the mixed material is cooled to 80-85°C and inserted into Bacillus subtilis natto, the inoculum amount is 6-8wt%, and fermented at 37-40°C for 66-72 hours;
[0053] Post-ripening: post-ripening the fermented product at 2-4°C for 12-24 hours.
[0054] Prefe...
Embodiment 1
[0064] A broad bean natto is prepared by fermenting 25wt% broad bean and 75wt% soybean through Bacillus subtilis natto.
[0065] The preparation method comprises the following steps:
[0066] Raw material screening: remove impurities and bad beans in broad beans and soybeans respectively;
[0067] Soaking: Wash the broad beans, cut the skin, add water three times the weight of the broad beans, and soak until there are no wrinkles on the surface; wash the soybeans, add water three times the weight of the soybeans, and soak for 24 hours at room temperature;
[0068] Cooking of raw materials: broad beans and soybeans are cooked with high-pressure steam; cooking conditions: 100°C, 20min.
[0069] Mixing: mixing 25wt% broad beans and 75wt% soybeans while hot;
[0070] Inoculation fermentation: the mixed material was cooled to 80°C and inserted into Bacillus subtilis natto, the inoculation amount was 6wt%, and fermented at 37°C for 72 hours;
[0071] Post-ripening: post-ripening ...
Embodiment 2
[0073] A broad bean natto is prepared by fermenting 50wt% broad bean and 50wt% soybean through Bacillus subtilis natto.
[0074] The preparation method comprises the following steps:
[0075] Raw material screening: remove impurities and bad beans in broad beans and soybeans respectively;
[0076] Soaking: Wash the broad beans, cut their skins, add water 4 times the weight of the broad beans, and soak until there are no wrinkles on the surface; wash the soybeans, add water 4 times the weight of the soybeans, and soak for 10 hours at room temperature;
[0077] Cooking of raw materials: broad beans and soybeans are cooked by high-pressure steam; cooking conditions: 121°C, 15min.
[0078] Mixing: mixing 50wt% broad beans and 50wt% soybeans while hot;
[0079] Inoculation fermentation: after cooling the mixed material to 85°C, insert Bacillus subtilis natto, the inoculation amount is 8wt%, and ferment at 40°C for 66 hours;
[0080] Post-ripening: post-ripening the fermented pro...
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