Anti-plant soft rot protein, coding gene of protein and built disease-resistant strain

A soft rot and plant technology, applied in the field of disease-resistant strains, can solve problems such as reducing the survival rate of banana plants, and achieve significant antibacterial effects, easy crystallization, and high stability

Active Publication Date: 2018-08-24
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the 1980s, it was reported that banana soft rot was endemic in the silted land of West Bengal, greatly reducing the survival rate of banana plants

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Anti-plant soft rot protein, coding gene of protein and built disease-resistant strain
  • Anti-plant soft rot protein, coding gene of protein and built disease-resistant strain
  • Anti-plant soft rot protein, coding gene of protein and built disease-resistant strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] One, the construction method of anti-plant soft rot protein and its genetic engineering strain:

[0024] 1. Amplify the target gene

[0025] According to the plasmid map of the plasmid, suitable enzyme cutting sequences are added to both ends of the artificial synthesis, respectively containing the nucleotide sequences of the genes shown in SEQ ID NO: 1 and SEQ ID NO: 3, and then recovered.

[0026] 2. Plasmid extraction and recombinant transformation

[0027] 2.1 Plasmid extraction

[0028] The strains storing the plasmids were activated in shake flasks and cultured for 12 hours. In order to prevent loss of the plasmids, 100 μL of antibiotics were added to 100 mL of LB liquid medium.

[0029] According to the operation method of the plasmid quick mini-extraction kit, the fresh bacterial liquid was subjected to the steps of harvesting, resuspension, lysis, neutralization, DNA binding, washing and elution in sequence for plasmid extraction, and the obtained plasmid was...

Embodiment 2

[0084] Shake flask fermentation:

[0085] Shake flasks to ferment pMK3BS-1 or pMK3BS-3 strains, the medium is 1.5% sucrose, 1.5% yeast extract powder, MgCl 2 0.5% and the balance in distilled water.

[0086] The optimum temperature for fermentation is 33-35°C, the optimum pH value is 7.0-7.2; the optimum liquid volume is 56mL / 500mL.

[0087] Fermentation tank fermentation:

[0088] Fermentation tank pMK3BS-1 or pMK3BS-3 strains, the medium is 0.75% sucrose, 0.75% corn starch, 0.75% yeast extract powder, 0.75% soybean cake powder, 0.3% MgCl 2 , 0.3%K 2 HPO 4 , 0.3% KH 2 PO 4 and the remainder of distilled water.

[0089] The optimum temperature for fermentation is 33-35°C, the optimum pH value is 7.0-7.2; the optimum stirring speed is 300r / min. During the fermentation process, sterile air was always introduced.

[0090] The growth of pMK3BS-1 or pMK3BS-3 strain in the fermentor was obviously better than that in the shake flask fermentation.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides an anti-plant soft rot protein, a coding gene of the protein and a built disease-resistant strain, and relates to an anti-plant-disease protein, a coding gene of the protein andthe built disease-resistant strain. The path for biologically preventing and treating the plant soft rot diseases is expanded; the novel plant soft rot prevention and treatment measure realizing zerowaste on production, zero ecological environment pollution on application, no adverse results on eating and no causing of bacterium drug resistance is provided. The amino acid sequence of the anti-plant soft rot protein is shown as SEQ ID NO:2; the nucleotide sequence of the gene is shown as SEQ ID NO:1; the amino acid sequence of the anti-plant soft rot protein is shown as SEQ ID NO:4; the nucleotide sequence of the gene is shown as SEQ ID NO:3.

Description

technical field [0001] The invention relates to a plant disease-resistant protein, its coding gene and a disease-resistant bacterial strain constructed therefrom. Background technique [0002] Plant bacterial soft rot, also known as rot, is a very important bacterial disease in the plant kingdom, and it occurs to varying degrees all over the world. Generally, water-soaked, light brown lesions appear at the initial stage after infection, and then the color gradually deepens, and the rot intensifies to complete lesions. [0003] Soft rot occurs in a wide range of varieties. Carrots, potatoes, cucumbers and other vegetable crops and most cruciferous vegetables and flowers, such as chrysanthemums, are all susceptible to soft rot. At the same time, the incidence range of soft rot is also very wide, and it may occur during various growth periods, storage and transportation of plants. The Yangtze River region of my country is a high-incidence area of ​​German iris soft rot. For v...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/24C12N15/31C12P21/02C12R1/125
CPCC07K14/24
Inventor 周晓辉何晓亮苗兰天刘浩
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap