Method for extracting plant polyphenol through combination of pulse electric field, double water phases and column chromatography

A technology of pulsed electric field and plant polyphenols, applied in plant raw materials, plant/algae/fungi/moss components, food science, etc., can solve problems such as high cost, low yield, complex process, etc., to improve extraction efficiency and product The effect of purity

Inactive Publication Date: 2018-08-28
TAIZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The invention provides a method for extracting plant polyphenols by combining pulsed electric field-two-phase-column chromatography, which couples a variety of advanced extraction methods, has the characteristics of high extraction efficiency and high purity of extracted products, and overcomes the existing The process has the problems of long process flow, complicated process, high cost and low yield

Method used

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  • Method for extracting plant polyphenol through combination of pulse electric field, double water phases and column chromatography
  • Method for extracting plant polyphenol through combination of pulse electric field, double water phases and column chromatography

Examples

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Embodiment 1

[0037] Embodiment 1: Utilize this process to extract bayberry polyphenols from red bayberry pulp

[0038] Waxberry (Myrica.rubra Sieb.et Zucc.) belongs to the Myrica family Myrica genus. The fruit is bright in color, juicy, sweet and sour, and has high nutritional value. Therefore, it is favored by consumers. It is concentrated from mid-to-late June to early-to-mid-July. At this moment, it is the rainy season in midsummer, and the market is concentrated, and it is extremely intolerant to storage and transportation. Red bayberry pulp is rich in polyphenols, so it can be considered to extract bayberry polyphenols from bayberry pulp for food antioxidants or biomedicine. combined with figure 1 , specify the processing steps of bayberry polyphenol extraction:

[0039] (1) Raw material pretreatment. Take 250g of bayberry frozen fruit, thaw it in the refrigerator at 5°C, wash it with water, remove the nucleus and beat it with a fruit and vegetable beater, then add 1L of 70% ethanol ...

Embodiment 2

[0044] Embodiment 2: Utilize this process to extract Cyclocaryaurol from Cyclocarya paliurus leaves

[0045] Cylocayapalirus, also known as the money tree, is a plant of the genus Cylocayapalirus in the family Juglandaceae. It is a rare tree species that integrates timber, greening, tea health care, and medicinal treatment. Cyclocarya paliurus leaves are rich in flavonoids, polysaccharides, sterols and other active organic ingredients, which have the effects of lowering blood sugar, blood fat, blood pressure, anti-aging, and anti-tumor. In recent years, it has received increasing attention in the fields of biomedicine and food health care. use as figure 2 The process flow shown is to extract polyphenols from Cyclocarya paliurus leaves.

[0046] (1) Raw material pretreatment. Take 20g of dried leaves of Cyclocarya paliurus, remove the stems, dry in a 65°C electric constant temperature blast drying oven for 6 hours, then pulverize with an ultrafine pulverizer, and pass throug...

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Abstract

The invention discloses a method for extracting plant polyphenol through the combination of a pulse electric field, double water phases and column chromatography. The method comprises the following steps of 1, pretreating plants to be extracted to obtain a raw material liquid; 2, with ethyl alcohol being solvent, utilizing the pulse electric field to extract active substances in the raw material liquid to obtain an extracting liquid; 3, after filtering the extracting liquid, adopting the double water phases to extract active components in the extracting liquid to obtain a plant polyphenol coarse extracting liquid; 4, after conducting rotary evaporation and alcohol removal on the plant polyphenol coarse extracting liquid, adopting a column chromatography method to purify the active substances in extract; 5, concentrating and drying to obtain a plant polyphenol product. Multiple advanced extracting methods are coupled, and the method has the advantages of being high in extracting efficiency and high in extracted product purity and solves the problems that an existing technology is long in technological process and complex, the component content is high, and the yield is low.

Description

technical field [0001] The invention belongs to the field of natural product chemistry and relates to methods for extracting, separating and purifying natural products, in particular to a method for extracting plant polyphenols by combining pulsed electric field-two aqueous phase-column chromatography. Background technique [0002] Plant polyphenols are polyphenolic secondary metabolites that widely exist in plants. They are rich in types, including anthocyanins, flavonols, flavonoids, gallic acid, and protocatechuic acid. With the discovery and elucidation of the activities of plant polyphenols in enzyme activity inhibition, anti-oxidation, anti-inflammation, antibacterial, anti-virus, anti-mutagenic, radiation protection, regulation of blood sugar and blood lipids, and improvement of immunity, plant polyphenols It is widely used in agriculture, ecological environment, food additives, biomedicine, cosmetics and other fields. As a pure natural extract obtained from nature, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/52A61K36/185A23L33/105
CPCA23L33/105A23V2002/00A61K36/185A61K36/52A61K2236/15A61K2236/17A61K2236/333A61K2236/51A61K2236/53A61K2236/55A23V2250/2132
Inventor 章志成袁玲姚俊孔庆娜
Owner TAIZHOU UNIV
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