Method for preparing RGD-polypeptide grafted poly(maleidohexanediamine acid-D,L-lactic acid)/beta-TCP composite material

A technology of adipamide acid and porous composite materials, applied in medical science, prostheses, etc., can solve the problems of loss of mechanical strength of matrix materials, excessive molecular weight drop, and different material properties, so as to achieve controllable reactions and products, The modification process is simple and the effect of improving mechanical properties

Inactive Publication Date: 2018-09-04
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After chemical modification, the modified product generally has a large molecular weight drop and loses the original mechanical strength of the matrix material; while the blended modified product has different properties due to uneven mixing.

Method used

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  • Method for preparing RGD-polypeptide grafted poly(maleidohexanediamine acid-D,L-lactic acid)/beta-TCP composite material
  • Method for preparing RGD-polypeptide grafted poly(maleidohexanediamine acid-D,L-lactic acid)/beta-TCP composite material
  • Method for preparing RGD-polypeptide grafted poly(maleidohexanediamine acid-D,L-lactic acid)/beta-TCP composite material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1) First, dissolve 2.335g of hexamethylenediamine in 10mL of dichloromethane, then add 6.106mL of triethylamine and stir evenly; dissolve 1.6335g of maleic anhydride in 5mL of dichloromethane. Under the condition of stirring at room temperature, the dichloromethane solution of maleic anhydride was added dropwise to the dichloromethane solution of hexamethylenediamine within 0.5h, and the reaction was continued at room temperature for 2h after the dropwise addition was completed. After the reaction is completed, the obtained mixed solution is separated by a THF-absolute ethanol co-precipitation method to obtain the maleamide hexamethylene diamine monomer, which is vacuum-dried for future use. The specific operation of the THF-absolute ethanol co-precipitation method is as follows: dissolve the reacted substance in an appropriate amount of THF, then drop excess absolute ethanol into the mixed solution, and collect the precipitate.

[0029] The infrared spectrogram of the ...

Embodiment 2

[0037] 1) First, dissolve 2.365g of hexamethylenediamine in 10mL of dichloromethane, then add 4.64mL of triethylamine and stir evenly; dissolve 1.25g of maleic anhydride in 5mL of dichloromethane. Under the condition of stirring at room temperature, the dichloromethane solution of maleic anhydride was added dropwise to the dichloromethane solution of hexamethylenediamine within 0.5h, and the reaction was continued at room temperature for 2h after the dropwise addition was completed. After the reaction is completed, the obtained mixed solution is separated by a THF-absolute ethanol co-precipitation method to obtain the maleamide hexamethylene diamine monomer, which is vacuum-dried for future use.

[0038] 2) Dissolve 5g of poly-D,L-lactide in an appropriate amount of tetrahydrofuran, and dissolve 0.504g of maleic hexamethylene diamic acid in an ethanol solution with a volume fraction of 90%. Under stirring conditions, drop the ethanol solution of maleic hexamethylene diamic aci...

Embodiment 3

[0042] 1) First, dissolve 2.5g of hexamethylenediamine in 10mL of dichloromethane, then add 5.9mL of triethylamine and stir evenly; dissolve 1.32g of maleic anhydride in 5mL of dichloromethane. Under the condition of stirring at room temperature, the dichloromethane solution of maleic anhydride was added dropwise to the dichloromethane solution of hexamethylenediamine within 0.5h, and the reaction was continued at room temperature for 2h after the dropwise addition was completed. After the reaction is completed, the obtained mixed solution is separated by a THF-absolute ethanol co-precipitation method to obtain the maleamide hexamethylene diamine monomer, which is vacuum-dried for future use.

[0043] 2) Dissolve 5g of poly-D,L-lactide in an appropriate amount of tetrahydrofuran, and dissolve 0.504g of maleic hexamethylene diamic acid in an ethanol solution with a volume fraction of 90%. Under stirring conditions, drop the ethanol solution of maleic hexamethylene diamic acid i...

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Abstract

The invention relates to a method for preparing a RGD-polypeptide grafted poly(maleidohexanediamine acid-D,L-lactic acid) / beta-TCP composite material. According to the method, maleidohexanediamine acid and RGD-polypeptide are adopted to modify polylactic acid, then cell affinity of the polylactic acid can be improved, and the polylactic acid is beneficial to adhesive growth of cells; hexamethylendiamine has the main function of controlling accumulation of acidity in the degradation process, and sterile necrosis of implanted tissue can be prevented; maleidohexanediamine acid is firstly synthesized, a polylactic acid side chain is further introduced, then the reaction times of polylactic acid can be reduced, molecular weight reduction of the polylactic acid can be avoided, and the mechanicalproperties of the composite material can be ensured; calcium and phosphorous ions generated from degradation of beta-TCP as a biodegradable material with good biocompatibility are also essential substances for growth of bone tissue, nerve cells and the like.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a method for preparing an RGD polypeptide grafted poly(maleyl adipamide-D, L-lactic acid) / β-TCP porous composite material that can be used for tissue defect repair. Background technique [0002] Peripheral nerve injury is a common clinical disease, and finding suitable repair materials has always been an urgent clinical problem to be solved. More and more attention has been paid to degradable polymer materials, such as polylactic acid (PLA), polyurethane (PU), polyamide (PA), among which polylactic acid and its copolymers with other materials are more commonly used. Polylactic acid has passed the FDA standard, because its degradation products are mostly small molecules and carbon dioxide, non-toxic, can be absorbed by the human body or excreted through metabolism, and because of its good biocompatibility and biodegradability, it is widely used in Tissue engineering,...

Claims

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Application Information

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IPC IPC(8): C08L87/00C08K3/32C08J9/28C08G81/02C08F283/02C08F222/38A61L27/56A61L27/54A61L27/50A61L27/46
CPCA61L27/46A61L27/50A61L27/54A61L27/56A61L2300/252A61L2300/604C08F283/02C08G81/02C08J9/0066C08J9/28C08J2387/00C08K2003/325C08F222/38
Inventor 王友法徐文磊
Owner WUHAN UNIV OF TECH
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