A hybrid photonic crystal composite material based on folic acid modification and its application

A technology of photonic crystals and composite materials, applied in animal cells, vertebrate cells, tumor/cancer cells, etc., can solve the problems of large damage to CTCs and limited capture efficiency of CTCs, and achieve high mechanical strength, superior magnetic response, and easy manipulation Effect

Active Publication Date: 2019-10-11
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, nanomaterials are used as carriers, and it is difficult to maintain a stable structure while having good biocompatibility. Therefore, the capture efficiency of CTCs based on these carriers is limited and the damage to CTCs is relatively large.

Method used

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  • A hybrid photonic crystal composite material based on folic acid modification and its application
  • A hybrid photonic crystal composite material based on folic acid modification and its application
  • A hybrid photonic crystal composite material based on folic acid modification and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Preparation of silica colloidal microspheres (SCCBs):

[0039] (1) SiO 2 After the nanoparticles were sonicated for 1 h, they were centrifuged at 8000 rpm for 10 min, and ultrapure water was added after removing the supernatant, and the above steps were repeated 20 times.

[0040] (2) SiO after the above cleaning 2 The nanoparticles were formulated into an emulsion with a mass fraction of 20%, and ultrasonicated for 30 minutes.

[0041] (3) Add an appropriate amount of n-hexadecane (containing 1% Hypermer2296 surfactant) to the collector and the oil phase injector, preheat at 45°C, adjust the oil phase speed to 2ml / h and the water phase speed to 1ml / h; wherein, Liquid volume of n-hexadecane: the distance from the bottom of the collector is about 0.3cm. If the amount is large, the balls will easily float and merge with each other. If the amount is small, the balls will not be in a suspended state and cause collapse; The amount of alkane is more than three times the to...

Embodiment 2

[0062] Preparation of silica colloidal microspheres (SCCBs):

[0063] Step is identical with embodiment 1.

[0064] Preparation of hybrid photonic crystal composites based on folic acid modification:

[0065](1) Preparation of pregel A: polyethylene glycol diacrylate 70%, photoinitiator 2% and water 28%; preparation of pregel B: methacrylate gelatin (GelMA), iron concentration is 4mg / ml Magnetic nano particle suspension, photoinitiator and water, wherein, the volume ratio of magnetic nano particle suspension, water and photoinitiator is 2:60:1, and magnetic nano particle is carboxylated ferroferric oxide magnetic nano particle.

[0066] (2) Preparation of inverse opal framework of polyethylene glycol diacrylate (PEGDA): Wrap the transparent plastic centrifuge tube containing SCCBs with tinfoil, add pre-gel A dropwise until it is completely covered, shake properly, and let stand at room temperature 45min, so that the pregel A is fully immersed in the pores of SCCBs; then use ...

Embodiment 3

[0070] Preparation of silica colloidal microspheres (SCCBs):

[0071] Step is identical with embodiment 1.

[0072] Preparation of hybrid photonic crystal composites based on folic acid modification:

[0073] (1) Preparation of pre-gel A: polyethylene glycol diacrylate 90%, photoinitiator 1% and water 9%; preparation of pre-gel B: methacrylate gelatin (GelMA), iron concentration of 4mg / ml Magnetic nano particle suspension, photoinitiator and water, wherein, the volume ratio of magnetic nano particle suspension, water and photoinitiator is 3:80:1.

[0074] (2) Preparation of inverse opal framework of polyethylene glycol diacrylate (PEGDA): Wrap the transparent plastic centrifuge tube containing SCCBs with tinfoil, add pre-gel A dropwise until it is completely covered, shake properly, and let stand at room temperature 30min, so that the pregel A is fully immersed in the pores of SCCBs; then use ultraviolet light to polymerize for 40s, during the polymerization process, the cen...

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Abstract

The invention discloses a mixed photonic crystal composite material based on folic acid modification and application. The mixed photonic crystal composite material is prepared from a mixed photonic crystal carrier and folic acid loaded on the carrier, wherein the mixed photonic crystal is prepared from polyethyleneglycol diacrylate inverse opal skeleton and methacrylate gelatin and magnetic nanoparticles with which skeleton pores are filled. The mixed photonic crystal composite material is applied to the detection for myeloid leukemia drug-resistance cells. The remarkable advantage of the mixed photonic crystal composite material is that the composite material is of a nano ordered structure, the specific surface area is large, the mechanical strength is high, and the stability is strong, and the composite material has the excellent biological compatibility and the magnetic responsiveness, and is easy to control. The composite material is applied to the detection for the drug-resistancecells, the myeloid leukemia drug-resistance cells can be specifically, sensitively, simply and effectively captured, and drug-resistance cell strains with good biological activity are guaranteed, andthe myeloid leukemia drug-resistance cells can be effectively enriched and separated after being captured only if a magnetic field is applied.

Description

technical field [0001] The invention belongs to the field of photonic crystals, in particular to a folic acid-modified mixed photonic crystal composite material and its application. Background technique [0002] Leukemia is one of the top ten malignant tumors. It is a malignant tumor of the blood system. Although great progress has been made in its diagnosis and treatment, and its five-year survival rate has also been greatly improved, its morbidity and mortality are still It is at a relatively high level, and it is showing an increasing trend in our country, so the prevention and treatment of leukemia is still a major issue facing our public health. The main treatments for leukemia are chemotherapy, targeted drug therapy, stem cell transplantation and immunotherapy. Among them, drug therapy is the cornerstone of leukemia treatment. Chemotherapy is required to induce remission before hematopoietic stem cell transplantation. Targeted drug therapy can greatly improve treatm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09
Inventor 陈宝安
Owner SOUTHEAST UNIV
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