44results about How to "Sensitive capture" patented technology

The invention provides a method and kit for sorting and / or enriching circulating tumor cells. According to the method, by utilizing an extremely and stable affinity among an anti-Her2 monoclonal antibody marked by biotin, an anti-EpCAM monoclonal antibody marked by biotin and an anti-Trop2 monoclonal antibody marked by biotin and magnetic beads covered with avidin, an immunomagnetic bead method is provided for sorting and / or enriching the circulating tumor cells. The invention further provides a kit used for sorting and / or enriching circulating tumor cells. The method for sorting and / or enriching the circulating the tumor cells can sensitively capture tumor cells, is applicable to conducting wide spectrum screening on the tumor cells, overcomes steric effect defects of common magnetic bead screening, and achieves the effects of efficiently capturing the tumor cells.

The invention provides a microbe and solidifying agent combined in-situ remediation method of heavy metal contaminated soil. The microbe and solidifying agent combined in-situ remediation method comprises the following steps: mixing magnetotactic bacteria and heavy metal contaminated soil; irrigating heavy metal contaminated soil mixed with magnetotactic bacteria; arranging a magnetic field on heavy metal contaminated soil; collecting heavy metal contaminated soil containing magnetotactic bacteria; leaching the collected heavy metal contaminated soil and back-filling; adding a solidifying agent into heavy metal contaminated soil subjected to magnetotactic bacteria adsorption treatment, and curing for 30-45 days; and circularly repeating the steps till the content of heavy metals in soil reaches the safe standard, wherein the solidifying agent consists of SiO2, Fe2O3, Al2O3 and C3N3S3Na3. The microbe and solidifying agent combined in-situ remediation method belongs to in-situ remediation, is simple to operate, low in cost and good in remediation effect, avoids secondary contamination of soil, and is suitable for remediation of large-area contaminated soil.

The invention relates to the technical field of an optical fiber induction layer. The optical fiber induction layer provided by the invention comprises an upper pressure-sensitive component thin film and a lower pressure-sensitive component thin film which are superposed, and at least one soft smooth thin film by which a space between the two layers of the pressure-sensitive component thin films is divided into at least two compartments; at least one optical fiber moving segment, which extends in a first direction and comprises a plurality of sections which are subjected to S-shaped serial wiring, is arranged in the same compartment, and wiring elbows of upper and lower adjacent optical fiber moving segments are superposed in a staggered mode. The optical fiber moving segments are uniformly distributed on the pressure-sensitive component thin films of the optical fiber induction layer, so that optical fiber induction regions are formed, and since the wiring elbows of the upper and lower optical fiber moving segments are superposed in a staggered mode, an induction blind area can be mutually filled up by the various optical fiber induction regions, so that the optical fiber induction layer, breaking through the characteristic that an optical fiber is easily broken, can be used for rapidly and accurately detecting breath, heartbeats, sleep, temperature and humidity; and the optical fiber induction layer has the characteristics of being high and adjustable in sensitivity, high in resolution, anti-electromagnetic-interference and uniform in sensitivity. The invention also provides a monitoring system, and the monitoring system comprises the optical fiber induction layer, a light source, a light detection component, an optical fiber transmission loop and a processing circuit.

The invention relates to photo-catalytic composite hydrogel based on cyclodextrin and a preparation method thereof. The cyclodextrin, nanometer titanium dioxide and acrylamide copolymer composite hydrogel synthesized by adopting an instant gel method has a three-dimensional net structure containing an amino group with a large number of chelate heavy metal ions, a carboxylate radical and nanometertitanium dioxide with photocatalysis. The photo-catalytic composite hydrogel based on the cyclodextrin, provided by the invention, can adsorb metal ions in water, is high in adsorption efficiency andhas wide application prospect in the fields of ion adsorption and industrial wastewater treatment.

The invention discloses a vacuum degree on-line detection method and device for a breaker and a potential information capturing sensor. Based on the vacuum insulation and discharge theory and the actual working conditions of a vacuum interrupter, the internal relation among the dielectric constant in the vacuum interrupter, the shielding case potential characteristic and the vacuum degree in the vacuum interrupter is built, and the vacuum degree in the vacuum interrupter can be obtained by measuring the shielding case potential information of the high-voltage vacuum breaker, so that on-line detection of the vacuum degree of the vacuum breaker can be realized.

The invention relates to the technical field of power plant running and maintenance, in particular to a running fault diagnosis system and method of power plant equipment. The running fault diagnosissystem comprises a front-end acquisition system, a relay transmission system and a background concentrated information processing system, wherein the front-end acquisition system comprises an inspection platform and a fixed detection platform and also comprises an industrial control module and an information acquisition module, the industrial control module and the information acquisition module are arranged on the inspection platform and the fixed detection platform, the information acquisition module is connected with the industrial control module, the industrial control module can be used for performing data transmission with the relay transmission system, and the relay transmission system is connected with the background concentrated information processing system. In the running faultdiagnosis system and method of the power plant equipment, provided by the invention, a fault state is effectively analyzed by a distributed architecture of an acquisition front end and a background concentrated information processing system.

The invention relates to a method for preparing multi-amino modified cyclodextrin, wherein the method comprises the steps for preparing multi-amino modified cyclodextrin in parts by mass: (1) dispersing cyclodextrin into water, and slowly adding 10%-15% strong alkali aqueous solution into the water until the system is transparent to obtain a solution I; (2) adding p-toluenesulfonyl chloride and acetonitrile into a reactor; dropwise adding the solution I; continuously carrying out a reaction for 3-5 h at room temperature after finishing dropwise adding for 0.5-1 h, ending the reaction, carryingout suction filtration, putting the filtrate into a refrigerator overnight, separating out a large number of white precipitates, carrying out suction filtration to obtain a white solid, dissolving the white solid with water, carrying out suction filtration, refrigerating the filtrate to separate out a large number of white precipitates, filtering, and carrying out vacuum drying to obtain poly-6-p-toluenesulfonyl cyclodextrin; and (3) dissolving the poly-6-p-toluenesulfonyl cyclodextrin and polyamine into a dipole solvent, carrying out a reaction at the temperature of 50-80 DEG C for 4-6 h, adding glycidyl methacrylate, continuing to carry out a reaction for 2-4 h, cooling to room temperature, adding methanol, adding acetone, precipitating, carrying out suction filtration to obtain a whitesolid, and repeating methanol dissolution and acetone precipitation to obtain the double-bond-containing multi-amino modified cyclodextrin.

The invention relates to the field of biochemical analysis and detection, and in particular relates to a hairpin probe group based on enzyme assisted cascade cycle amplification, a preparation methodand use. According to the hairpin probe group provided by the invention, a biological target to be detected is used as a first-level target to trigger first target cycle amplification, so that enrichment amplification of a secondary target sequence is realized; simultaneously, the secondary target sequence also can trigger second target cycle amplification; fluorescent signal sequences are continuously released; and thus, cascade cycle amplification of the fluorescent signal sequences is realized. By adoption of the hairpin probe group, a lot of fluorescent signal sequences can be triggered and generated only through trace biological targets; the very high fluorescence signal intensity is generated; the detection sensitivity of the biological target is obviously increased; the detection limit is increased; simultaneously, the specificity of the cascade cycle amplification of the probe in combination with the biological target is high; and a detection result is accurate.

The invention provides a manufacturing method of a semiconductor device test sample. The manufacturing method comprises the steps as follows: a substrate is provided; the substrate is subjected to at least one-time oxidation treatment, the oxidation treatment comprises the steps of forming of an oxidation layer on the substrate surface and removing of the oxidation layer, and after the oxidation treatment, when the substrate has a dent mark defect, the size of the dent mark defect is increased; and the test sample is detected, and wafer detection equipment can flexibly capture the defect of a dent mark on the substrate surface on line and timely monitors the defect.

The invention discloses an omni-directional wind speed sensor based on a NTC thermistor. The omni-directional wind speed sensor based on the NTC thermistor comprises a top hood, a cylindrical NTC thermistor, a packaging sensor base, a connecting assembly, a circuit board, a connecting arm, an insulating cap and a cable, wherein a threaded hole is formed in one end of the top hood; outer threads, which are matched with the threaded hole, are arranged at one end, which is close to the top hood, of the connecting assembly; the top hood is connected with the connecting assembly through the outer threads and the threaded hole; and a cavity structure is formed in the middle of the connecting assembly. An end of the cylindrical NTC thermistor is protected by the top hood, meanwhile, air motion in various directions is not affected, the cylindrical NTC thermistor with resistance of 2K ohm is used as a wind sensing probe, large change of a resistance value is realized by low power consumption, so that weak wind speed can be responded quickly, and by a high-precision constant-current source and the combination of an analog amplifying circuit and a high-speed digital acquiring circuit, the weak wind speed can be sensitively captured.

The invention discloses a highly selective, highly sensitive, and universal cell-specific capture device and method. The nucleic acid aptamer of the invention is immobilized at one time, which can achieve high selectivity and high specificity for target cancer cells Capturing and providing a simple and easy elution method. The capture method of the present invention does not require complicated surface modification process, is low in cost, takes less time, is simple to operate, and has extremely wide application prospects.

The invention discloses a cell analysis method based on cell nucleus DNA analysis, computer equipment and a storage medium. The cell analysis method comprises a first appearance form judgment step and a second appearance form judgment step; the first appearance form judgment step is realized by obtaining a parameter of the area size of a cell nucleus, defining the parameter as a DNA parameter 1, respectively presetting a threshold value A, a threshold value B and a threshold value C for the DNA parameter 1, and dividing the DNA parameter 1 into four numerical ranges, thereby dividing the cells to be analyzed into four types: abnormal cells, suspicious abnormal cells, highly suspicious abnormal cells and positive abnormal hyperplasia cells; and further calculating and judging the coincidence probability of the four types of cells by utilizing a second appearance form judgment step. Therefore, by utilizing the method disclosed by the invention, the speed of cell analysis results can be increased, the workload of medical personnel can be reduced, the accuracy of the output analysis results can be ensured, and the problems of missed diagnosis, misdiagnosis and the like can be avoided.

The invention discloses a device and method for sensitively detecting a film penetration pressure value in a film distillation process, wherein the device comprises a gas storage tank, a film penetration test assembly, a direct current power supply, a high-sensitivity electric bridge and a diaphragm to be tested, wherein the film penetration test assembly comprises an accommodating barrel, a flange plate and a bottom plate; the diaphragm to be tested is positioned between the flange plate and the bottom plate; a conducting sheet is arranged between the diaphragm to be tested and the bottom plate; the gas storage tank is connected with the accommodating barrel through a pressure reducing valve, a pressure regulator and a pressure meter; an upper binding post and a lower binding post are further arranged on the upper end of the accommodating barrel and the lower side of the conducting sheet; the direct current power supply is connected with the upper binding post and the lower binding post; two conducting posts are further distributed on the side wall of the accommodating barrel; and the high-sensitivity electric bridge is connected with the two conducting posts. By using the method,the device is used for performing film penetration pressure detection. According to the device and the method provided by the invention, the film soaking phenomenon can be reflected through the electric parameter detection of the film assembly, so that the maximum operation pressure capable of being born by the system can be fast and accurately indicated.

The invention discloses a filter quantity meter. The filter quantity meter comprises a vibration sensor and a metering mechanism. The vibration sensor comprises an output end and a sensing end capable of sensing vibration caused by a to-be-metered medium. The output end of the vibration sensor is connected with the input end of the metering mechanism. The invention further discloses a filter quantity metering method, a filter element with a metering function and a filter with a metering function. The filter quantity meter has the advantages that the small vibration sensor is used, the filter quantity meter is isolated from the to-be-metered medium and does not directly contact with the to-be-meter medium, the filter quantity meter can sensitively capture the vibration caused by the to-be-meter medium, and various parameters of the to-be-meter medium can be metered; the metering mechanism is arranged externally, the liquid to-be-metered medium cannot destroy the metering mechanism easily, water-electricity isolation can be achieved effectively, and use safety is achieved.

The invention relates to an antibacterial photocatalyst composite hydrogel for removing heavy metals, wherein the antibacterial photocatalyst composite hydrogel is cyclodextrin, nano titanium dioxideand acrylamide copolymer composite hydrogel synthesized by adopting an instantaneous gel method, has a three-dimensional network structure, and structurally contains amino groups and carboxylate radicals of a large amount of chelated heavy metal ions and nano titanium dioxide with a photocatalyst effect. The antibacterial photocatalyst composite hydrogel for removing the heavy metals can adsorb metal ions in water and is high in adsorption efficiency; wide application prospects are realized in the fields of ion adsorption and industrial sewage treatment.

The invention discloses a mixed photonic crystal, and a preparation method and applications thereof. The mixed photonic crystal comprises a polyethylene glycol 200 diacrylate inverse opal skeleton andmetacrylic acid ester gelatin and magnetic nanoparticles used for filling apertures of the skeleton. According to the preparation method, silica colloidal microspheres are taken as a template, a pre-gel A is added for reaction and polymerization, and the silica colloidal microspheres are removed so as to obtain the polyethylene glycol 200 diacrylate inverse opal skeleton; pre-gel B is added intothe polyethylene glycol 200 diacrylate inverse opal skeleton for reaction and polymerization so as to obtain a finished product. The mixed photonic crystal can be used in drug resistance cell detection as a carrier. The mixed photonic crystal is high in stability, possesses excellent biocompatibility and magnetic responsiveness; the preparation method is simple; the operationality is high; the preparation method is friendly to the environment; the mixed photonic crystal can be used in drug resistant cell detection; the surface of the mixed photonic crystal can be loaded with folic acid; and simple and effective capturing of myeloid leukemia drug resistance cells can be realized with high specificity and sensitivity.

The invention provides a sensing wire for fitness sportswear which belongs to the field of wires. The sensing wire provided by the invention comprises a core wire and a sensor element; and the core wire comprises an inner core wire and a wire wrapper, and the sensor element comprises a gold-microfilament semi-embedded substrate and a lead. The gold-microfilament semi-embedded substrate adopts a three-wire-system connection way, the top of the lead is coated with conductive silver paste so as to fix the lead, and a connection way between the core wire and the sensor element is to adopt resistance soldering between the inner core wire of the core wire and the corresponding lead of the sensor element. The sensing wire for the fitness sportswear provided by the invention is long in service life and wide in applicability, the 1000-time stretching recovery rate is only 1.84 percent, and meanwhile, the sensing wire can still work normally when being stretched by 3.1cm.

The invention discloses an anti-theft transformer, which includes uprights, steel frames are fixedly connected between the uprights, and an induction box is fixed on the upper end of the steel frames; When taking the transformer, it emits bright lights and harsh alarm sounds to warn offenders, stop illegal activities in time, and avoid excessive damage to transformer-related equipment. Using counter-control motors and cameras, illegal personnel ignore sound and light warnings , when attempting to violently steal the transformer, the visual image of the offender is captured and recorded in time, and the camera device is also prevented from being damaged. In windy weather or other violent vibrations, the source of external force can be detected sensitively to avoid false activation of the anti-theft device, resulting in excessive use of equipment and saving costs.

The invention discloses a device and method for sensitively detecting membrane permeation pressure value in membrane distillation process, wherein, the device includes a gas storage tank, a membrane permeation test component, a DC power supply, a high-sensitivity electric bridge and a diaphragm to be tested; the membrane The soaking test assembly includes an accommodating cylinder, a flange plate and a bottom plate, the diaphragm to be tested is located between the flange plate and the bottom plate, and a conductive sheet is arranged between the diaphragm to be tested and the bottom plate; the gas storage tank is reduced The pressure valve, the pressure regulator and the pressure gauge are connected to the storage cylinder; the upper terminal and the lower terminal are also arranged on the upper end of the storage cylinder and the lower side of the conductive sheet, and the DC power supply is connected with the upper terminal and the lower terminal. The connecting posts are connected; two lead posts are distributed on the side wall of the containing cylinder, and the high-sensitivity electric bridge is connected with the two lead posts; the method is to use the above-mentioned device to detect the membrane penetration pressure value. The invention can reflect the membrane wetting phenomenon through the electrical parameter detection of the membrane module, so as to quickly and accurately indicate that the system can bear the maximum operating pressure.

The invention discloses a tuning fork density meter and its coefficient calibration method. The tuning fork density meter includes a transmitter, a tuning fork sensing device, a connecting column connecting the transmitter and the tuning fork sensing device, and a bubble eliminating device. The tuning fork sensing device includes a tuning fork seat fixed on the quick-connect flange of the connecting column, a tuning fork body integrally connected with the tuning fork body, a strain gauge arranged at the inner end of the bottom hole at the root of the tuning fork body, and A temperature sensor, the tuning fork sensing device is arranged inside the air bubble eliminating device. Compared with the prior art, the strain gauge is closely attached to the root of the tuning fork fork body, which can more sensitively capture the change signal of the resonant frequency of the tuning fork fork body, and improves the accuracy and stability of density measurement. Calibrate the coefficient of the tuning fork density meter, eliminate the interference of external factors (such as temperature, current, etc.) on the density meter, and improve the accuracy of measurement.

The invention provides a non-volatile echo wall mode all-optical switch based on a Ge2Sb2Te5 phase change material and a preparation method thereof. The preparation method comprises the following steps that S1, an echo wall mode initial optical fiber microsphere resonant cavity with the diameter of 125 [mu] m is prepared by using a standard single-mode optical fiber; S2, a Ge2Sb2Te5 phase change material with the thickness of 50 nm + / -1 nm is sputtered on the optical fiber microspheres through a magnetron sputtering system, and a Ge2Sb2Te5 functionalized microsphere resonant cavity is prepared; and S3, the optical fiber probe guides 532nm pulse laser with different powers and a constant pulse number to carry out optical switch modulation on the Ge2Sb2Te5 functionalized microsphere resonant cavity. According to the invention, by means of the advantages of small size and easy integration of the optical fiber, cascade and integration of the material echo wall mode optical fiber microspheres based on the GST phase change material can be realized, collaborative regulation and control of multiple all-optical switches are realized, and an all-optical network switch system is constructed.

The invention discloses a mixed photonic crystal composite material based on folic acid modification and application. The mixed photonic crystal composite material is prepared from a mixed photonic crystal carrier and folic acid loaded on the carrier, wherein the mixed photonic crystal is prepared from polyethyleneglycol diacrylate inverse opal skeleton and methacrylate gelatin and magnetic nanoparticles with which skeleton pores are filled. The mixed photonic crystal composite material is applied to the detection for myeloid leukemia drug-resistance cells. The remarkable advantage of the mixed photonic crystal composite material is that the composite material is of a nano ordered structure, the specific surface area is large, the mechanical strength is high, and the stability is strong, and the composite material has the excellent biological compatibility and the magnetic responsiveness, and is easy to control. The composite material is applied to the detection for the drug-resistancecells, the myeloid leukemia drug-resistance cells can be specifically, sensitively, simply and effectively captured, and drug-resistance cell strains with good biological activity are guaranteed, andthe myeloid leukemia drug-resistance cells can be effectively enriched and separated after being captured only if a magnetic field is applied.

The invention discloses an intelligent closestool flushing system based on a UWB positioning technology, which comprises a base station, a positioning label and a control device, and is characterized in that the control device comprises a CPU control unit, a closestool control unit and a power supply module, and the power supply module is used for supplying power to the control device and the base station; wherein the base station and the positioning label are in signal communication, the base station is electrically connected with the control device, and the CPU control unit is used for controlling opening and closing of a toilet lid and flushing of a toilet through the toilet control unit. According to the intelligent closestool flushing system based on the UWB positioning technology, the corresponding flushing amount can be intelligently provided, the dynamic state of people is sensitively captured, and the sensing capacity of a closestool is improved; and moreover, energy is saved, and the limitation problem of the toilet position of people can be reasonably improved according to the AOA and TOF technologies of UWB.

The invention discloses a double-hearth lime kiln suspension cylinder temperature monitoring method and system and a storage medium. The double-hearth lime kiln suspension cylinder temperature monitoring method comprises the following steps that S1, the air temperature ti of each temperature measuring point i in an airflow cooling flow channel of a suspension cylinder is obtained; s2, the estimated average wall surface temperature T'w of the suspension cylinder is obtained through calculation; s3, calculating logarithmic temperature rise aj of the temperature measurement interval j; and according to the logarithmic temperature rise curve corresponding to the logarithmic temperature rise aj, determining that the logarithmic temperature rise aj deviating from a preset threshold value on the logarithmic temperature rise curve is abnormal temperature rise, and obtaining a temperature measurement interval corresponding to the abnormal temperature rise as an overtemperature area. According to the double-hearth lime kiln suspension cylinder temperature monitoring method, temperature rise signals between local overtemperature areas are dominantly changed by using a mean value checking method and logarithmic temperature rise changes, the local overtemperature areas are determined, the capture and monitoring of the local overtemperature of the suspension cylinder are more sensitive, timely early warning can be provided for a production field, and the production efficiency is improved. Therefore, the service life of the suspension cylinder is effectively prolonged.

The invention discloses an anti-theft transformer. The anti-theft transformer comprises stand columns, a steel frame is fixedly connected between the stand columns, and an induction box is fixedly arranged on the upper end face of the steel frame; through cooperation of the alarm motor and the sound-light alarm assembly, when a law-breaking person tries to steal the transformer, bright light and harsh alarm sound are given out, the law-breaking person is warned, illegal behaviors are stopped in time, excessive damage of the law-breaking person to related equipment of the transformer is avoided, and by means of the counter motor and the camera, when a law-breaking person does not take care of acousto-optic warning and attempts to steal the transformer violently, visual images of the law-breaking person are shot and recorded in time, the camera device is prevented from being damaged, meanwhile, the shooting device does not need to be connected with a power source when being in a standby state, energy is saved, and by means of cooperation of a buffer block and a buffer rod, in windy weather or other strenuous vibration, the external force source is sensitively captured, the anti-theft device is prevented from being mistakenly started to cause overuse of equipment, and the cost is saved.

The invention discloses a double-hearth lime kiln suspension cylinder temperature monitoring method and system and a storage medium. The double-hearth lime kiln suspension cylinder temperature monitoring method comprises the following steps that S1, the air temperature ti of each temperature measuring point i in an airflow cooling flow channel of a suspension cylinder is obtained; s2, an estimated heat transfer constant const is obtained; s3, obtaining the proportional temperature rise aj of the temperature measurement interval j; and according to a proportional temperature rise curve corresponding to the proportional temperature rise aj, determining that the proportional temperature rise aj deviating from a preset threshold value on the proportional temperature rise curve is abnormal temperature rise, and obtaining an area corresponding to the abnormal temperature rise as an overtemperature area. According to the method for monitoring the temperature of the double-hearth lime kiln suspension cylinder, the abnormal temperature rise is dominantly changed through the mean value checking method and the change of the proportional temperature rise, the local overtemperature area is determined, capturing and monitoring of the local overtemperature of the suspension cylinder are more sensitive, and timely early warning can be provided for a production site, so that the service life of the suspension cylinder is effectively prolonged.

The invention relates to a cold-rolled silicon steel quality defect prediction method based on a neural network, and the method comprises the following steps: S1, carrying out the full-flow material tracking of cold-rolled silicon steel, completing the collection of the process data of each unit experienced by a steel coil and the collection of the silicon steel quality defect result, and carrying out the length mapping between the units, obtaining the length position of each unit corresponding to each part of the raw material and the process parameter and quality defect result at the position; s2, determining influence factors of cold-rolled silicon steel quality defects, and constructing a data set; S3, performing K-means clustering analysis on the input items of the data set; S4, selecting a data set with the most characteristic from the clustered data; S5, establishing BP neural network training data; and S6, predicting the cold-rolled silicon steel quality defect probability by using a neural network. According to the method, the technical bottleneck in the mechanism model research process is overcome, and the influence of the change of the process parameters on the quality defect can be better and more sensitively captured.