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Anticoagulant and application thereof

An anticoagulant and catheter technology, applied in the biological field, can solve problems such as catheter blockage and no anticoagulant catheter, and achieve the effects of preventing blood adhesion, low cost in the preparation process, and reducing friction

Inactive Publication Date: 2018-09-28
北京康博特森科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in some animal surgery experiments, especially in catheterization experiments, catheter blockage is one of the main reasons for the failure of the experiment. So far no effective anticoagulant has been found to prevent catheter blockage

Method used

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  • Anticoagulant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, preparation and concentration comparison of anticoagulant

[0031] 1. Dissolve sodium chloride in distilled water to make physiological saline solution for later use.

[0032] 2. Weigh heparin sodium and dissolve it in normal saline to make base solution.

[0033] 3. Use a graduated cylinder to measure glycerol, dissolve it in the base solution, and mix thoroughly.

[0034] 4. Weigh PVP, trisodium citrate, EDTA, and magnetic stirrer to fully mix and dissolve to make an anticoagulant.

[0035] Six different concentrations of anticoagulants were prepared by the above method:

[0036] Anticoagulant A, the prepared anticoagulant comprising mass percent are 34% glycerol, 1.4% PVP, 1.5% trisodium citrate, 0.1% EDTA, 0.5% sodium chloride, 0.09% heparin sodium, and the balance is water.

[0037] Anticoagulant B, the prepared anticoagulant comprising mass percent are 18% glycerol, 3.8% PVP, 0.2% trisodium citrate, 0.6% EDTA, 0.7% sodium chloride, 0.02% of hepa...

Embodiment 2

[0054] Embodiment two, compare with single-component anticoagulant

[0055] In order to verify the effect, three common single-component blood anticoagulants, namely sodium heparin, EDTA, and trisodium citrate, were prepared in addition, and compared with the anticoagulant C in Example 1 for the catheter anticoagulant effect test.

[0056] 1. Take a 24-well plate, divide the 24 wells into 4 groups, each group has 6 wells, inject the above 4 groups of anticoagulants into the 4 groups of wells, and mark them well. Place a small section of silicone tubing in each well and soak for 10 minutes.

[0057] 2. Take out the soaked silicone tube, blot the surface liquid, put it into another clean 24-well plate, and mark it.

[0058] 3. Take 1 rat, remove the eyeballs and take blood, drop 3 drops of blood into each hole, and make the blood fully mix with the silicone tube.

[0059] 4. After soaking in the blood for 10 minutes, place the silicone tube on the surgical drape to fully ensur...

Embodiment 3

[0063] Embodiment 3, the experiment of inserting the silicone tube soaked in anticoagulant

[0064] Experimental animals: 27 healthy wild-type SPF grade, 8-week-old C57 mice.

[0065] Mouse carotid artery cannulation steps:

[0066] 1) Sterilize surgical instruments with high temperature and high pressure steam.

[0067] 2) Arterial catheter preparation: PE-10 catheter (about 1.2cm in length) was inserted into a 0.02-inch inner diameter silicone tube (about 5cm in length), and alcohol-sterilized for later use.

[0068] 3) Pentobarbital sodium was injected intraperitoneally, and the mouse was fixed in the supine position after anesthetization.

[0069] 4) Shave the surgical site on the neck and disinfect the surgical site with alcohol.

[0070] 5) Make a longitudinal incision about 1 cm long on the midline of the mouse neck, bluntly separate the left carotid artery with forceps, and separate it from the vagus nerve with a glass minute needle, ligate the distal end of the car...

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Abstract

The invention provides an anticoagulant which comprises, in weight percent, 18-34% of glycerol, 1.4-3.8% of PVP (polyvinyl pyrrolidone), 0.2-1.5% of trisodium citrate, 0.1-1% of EDTA (ethylene diaminetetraacetic acid), 0.5-0.7% of sodium chloride, 0.02-0.09% of heparin sodium and the balance water. The invention further provides an application of the anticoagulant to catheter anticoagulation. Various components are reasonably proportioned to prepare the anticoagulant, the preparation process is low in cost and rapid, catheter obstruction caused by coagulation of blood in a catheter can be stopped, the problem of incapability of effectively performing catheter anticoagulation by a common single-component anticoagulant is solved, and the anticoagulant can be widely applied to an animal catheterizing operation experiments.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an anticoagulant and its application in catheter anticoagulation. Background technique [0002] The application of physical or chemical methods to remove or inhibit certain coagulation factors in the blood to prevent blood coagulation is called anticoagulation. The light yellow liquid in the upper layer after centrifugation is plasma. Chemical agents or substances that prevent blood from clotting are called anticoagulants or anticoagulant substances. As far as is known, a variety of substances have been found to have targeted effects on blood anticoagulation, such as natural anticoagulants (heparin, hirudin, etc.), Ca 2+ Chelating agents (sodium citrate, potassium fluoride), commonly used anticoagulants are EDTA (ethylenediaminetetraacetic acid), heparin, citrate, etc. [0003] EDTA is one of the most commonly used and important anticoagulants and reagents in clinical work. Its me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L33/10A61L33/06A61L33/04A61L33/00A61L33/02
CPCA61L33/0011A61L33/027A61L33/04A61L33/064A61L33/08A61L2300/42C08L39/06C08L5/10
Inventor 魏巧芳
Owner 北京康博特森科技有限公司
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