Method for separating chiral eriodictyol from peanut shells and application of chiral eriodictyol

A peanut shell and eriodictyol technology, which is applied in the field of separation of flavonoids chiral eriodictyol in peanut shells, can solve the problems of high price and limited application of chiral columns, and achieve improved comprehensive utilization and low dosage , The effect of short component analysis time

Active Publication Date: 2018-10-09
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, studies have reported that many enantiomer separation methods of chiral drugs, such as high performance liquid chromatography (HPLC), gas chromatography (GC), thin layer chromatography (TLC), supercritical fluid chromatography (SFC), etc. respective disadvantages
The chiral columns used in HPLC and GC are expensive, and in most cases, a specific chiral column can only analyze a certain type of substance, and its application is often limited

Method used

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  • Method for separating chiral eriodictyol from peanut shells and application of chiral eriodictyol
  • Method for separating chiral eriodictyol from peanut shells and application of chiral eriodictyol
  • Method for separating chiral eriodictyol from peanut shells and application of chiral eriodictyol

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specific Embodiment approach 1

[0019] Specific implementation mode one: what this implementation mode records is a kind of method for separating chiral eriodictyol in peanut shells, and the described method steps are as follows:

[0020] Step 1: Processing of peanut shells: remove impurities, wash, and dry until the surface is dry or there is no clear water on the surface, then crush and pass through a 40-100 mesh sieve;

[0021] Step 2: Add 10-20 times the weight of 40-90wt.% ethanol to the peanut shell powder for reflux extraction for 1-3 times, 1-2h / time, and combine the extracts;

[0022] Step 3: Concentrate the extract from step 2 and use acetic acid to adjust the pH to be weakly acidic, add deionized water to dilute, pass through a macroporous resin column, quickly elute with water to remove impurities, then add 60~90wt.% ethanol to elute and collect The eluate was concentrated to obtain the purified product;

[0023] Step 4: Prepare ammonium acetate buffer solution with a pH of 8.0~9.0, add MAH-β-CD...

specific Embodiment approach 2

[0025] Specific embodiment two: a method for separating chiral eriodictyol in peanut shells described in specific embodiment one, in step two, 15 times the weight of 60wt.% ethanol is added to the peanut shell powder for reflux extraction twice , 1~2 h / time, combine the extracts; in step 3, concentrate the extract in step 2 to 60% of the original volume, add acetic acid to adjust the pH to 5.5, add deionized water to dilute 2~4 times, pass 0.10 ~0.20 g medicinal material / mL resin column, quickly eluted with water to remove impurities, then added 60~90wt.% ethanol for elution, collected the eluate, concentrated to 20~50% of the original volume, and obtained the purified product.

specific Embodiment approach 3

[0026] Embodiment 3: The method for separating chiral eriodictyol in peanut shells described in Embodiment 1, in step 3, the macroporous resin column is HPD300, HPD500 or D101.

[0027] Embodiment 4: A method for separating chiral eriodictyol in peanut shells described in Embodiment 1. In step 4, the synthesis method of the MAH-β-CD is to weigh maleic anhydride dissolved In dimethylformamide (DMF), add β-CD with stirring at room temperature, and react in a constant temperature water bath at 80°C±2°C for 8-12 hours. After the reaction, the product is cooled to room temperature, and the product is precipitated with chloroform. Wash thoroughly with acetone, drain with a suction filter bottle, dry in vacuum, and set aside.

[0028] Specific embodiment five: a method for separating chiral eriodictyol in peanut shells described in specific embodiment four, the synthetic method of the MAH-β-CD is to take 5.0 g maleic anhydride and dissolve it in 30 Add 5.6 g β-CD to mL DMF with stir...

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Abstract

The invention discloses a method for separating a flavonoids compound chiral eriodictyol from peanut shells and application of the chiral eriodictyol, and belongs to the technical field of plant extraction and separation drugs. The method comprises the following steps: treating the peanut shells; performing reflux extraction on peanut shell powder with ethyl alcohol for once to three times to obtain a concentrated solution; concentrating an extracting solution, adjusting the pH, diluting the solution, filtering the solution through a macroporous resin column, performing water elution and ethanol elution, collecting elutent, and concentrating the eluent to obtain a purified substance; preparing ammonium acetate, adjusting the pH of the system, and adding MAH-beta-CD into the prepared buffersolution for mixing, dissolving and volume metering to obtain a running buffer solution; filtering the purified substance with a filter membrane, and separating the purified substance by using a capillary electrophoresis method to obtain two eriodictyol enantiomers of R-structured 2R-eriodictyol and S-structured 2S-eriodictyol. The method disclosed by the invention has the advantages that the comprehensive utilization rate of the peanut shells is increased, the extraction and separation operation is simple, the analysis time is short, and the efficiency is high; by selection of a proper chiral selective agent, the separation efficiency is high, the use amount of the chiral selective agent is small, the cost performance is high, and the separation degree is up to 4.17.

Description

technical field [0001] The invention belongs to the technical field of extracting and separating medicines from plants, and in particular relates to a method for separating chiral eriodictyol, a flavonoid compound in peanut shells, and an application thereof. Background technique [0002] Peanuts are derived from the dried pods of peanuts, an annual herbaceous plant of the family Fabaceae, and the peanut shell is the shell of the pods. my country's peanut shells are rich in resources and low in price, and have broad application prospects in the fields of medicine, industry, agriculture, and health food. Peanut shell is a Chinese herbal medicine in the folk, which can treat asthma, phlegm, high blood pressure and so on. The flavonoids in peanut shells have various pharmacological activities such as lowering blood pressure, lowering blood fat, dilating coronary arteries, anti-oxidation, antitussive, expectorant and asthma, anti-bacterial and anti-inflammatory, enhancing immun...

Claims

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Application Information

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IPC IPC(8): C07D311/32C07D311/40C07D311/30A61P9/00A61P11/00
CPCA61P9/00A61P11/00C07B57/00C07D311/30C07D311/32C07D311/40C07B2200/07
Inventor 徐蕾高立娣陈林刘树仁王宁
Owner QIQIHAR UNIVERSITY
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