Bismuth, copper mixed coating test strip and its preparation method and application

Abstract

The invention relates to a bismuth-copper hybrid coating test strip and a preparation method and application thereof. The preparation method includes the steps of S101 adding buffer solution to bloodsample, then adding blood digestion liquid and mixing uniformly to obtain pretreatment blood sample, wherein the raw material component of the blood digestion liquid includes one or several of TritonX-100, SDS (Sodium Dodecyl Sulfonate), proteinase K and concentrated nitric acid; S102 placing the bismuth-copper hybrid coating test strip in the pretreatment blood sample, and performing anodic teststripping voltammetry to obtain lead content data of the blood sample. According to the preparation method, bismuth and copper serve as main materials, and an environment-friendly bismuth / copper hybrid pre-coating printing test strip is developed, thus the using of mercury membrane electrode in a micro electrochemical detection device is replaced; moreover, the bismuth / copper printing test striphas the advantages of high reliability, high selectivity and high utility, and is capable of detecting the concentration of lead ions in blood rapidly and accurately, and the amount of the blood is less, the operation of treating blood is easy, and the manufacture cost is low.

Description

technical field [0001] The invention relates to the technical field of electrochemical detection, in particular to a bismuth and copper mixed coating test strip, a preparation method and application thereof. Background technique [0002] Lead is the only trace element that the human body does not need. Lead and lead compounds pose a great threat to human health and can enter the human body through the digestive system and respiratory system. With the rapid development of industrialization, lead pollution in the environment is becoming more and more serious. After lead is absorbed into human blood, it will quickly deposit into soft tissue and enter bone tissue. Its residual time is as long as ten years, and it will pose a threat to all human organs, especially for children (damage to nerves and intelligence). Even if people get out of the lead-polluted environment or undergo treatment to significantly reduce the blood lead level, the damage caused by lead to human organs is...

AI Technical Summary

Problems solved by technology

While these methods can obtain reliable test results, they also have certain defects, such as low lead atomization rate, strict requirements on sample processing and working environment, secondary pollution caused by sample processing and nitrification process, need for professionals, measurement accuracy and range There are limitations, not suitable for large-scale sample screening, etc.

Existing commercialized blood lead analyzers (such as ESA Company, etc.) mainly use electrochemical analysis methods, such as square wave voltammetry, potential stripping method, etc., which have small volume and low consumption compared with traditional electrochemical workstations. It has the advantages of less time and high detection accuracy, but its application range is still in hospitals, disease control centers and research centers, while domestic instruments are only suitable for laboratory environments, and there are problems such as backward technology, large size, and inconvenient operation, so they are not suitable for clinical practice. And portable use and requirements, it is more difficult to apply to the deletion of large batches of samples

Most of the electrodes used in this type of instrument are mercury film electrodes, and the preparation process is complicated and the cost is high. At the same time, mercury is a heavy metal harmful to the human body and needs to be strictly controlled.

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