Method for efficiently and rapidly preparing free astaxanthin

A kind of astaxanthin, fast technology, applied in the biological field, can solve the problems of long reaction time, difficult to control the product, low hydrolysis efficiency of free astaxanthin, etc., and achieve the effect of improving low efficiency and increasing conversion rate

Active Publication Date: 2018-10-12
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the hydrolysis of astaxanthin ester mainly adopts the saponification method. The saponification method takes a long time to react, the reaction process is too violent, and the product is difficult to control. Moreover, high-concentration alkali will damage astaxanthin and produce more similar astaxanthin and semi-astaxanthin. By-products, and the alkali and organi

Method used

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  • Method for efficiently and rapidly preparing free astaxanthin
  • Method for efficiently and rapidly preparing free astaxanthin
  • Method for efficiently and rapidly preparing free astaxanthin

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Example 1

[0032] Screening of strains that hydrolyze astaxanthin esters:

[0033] The medium formula used in this example is as follows:

[0034] Screening flat solid medium:

[0035] Haematococcus pluvialis oil, 0.05%; Triton X-100, 1.0%; KNO 3 , 0.1%; K 2 HPO 4 , 0.05%; MgSO 4 ·7H 2 O, 0.05%; NaCl, 0.05%; FeSO 4 ·7H 2 O, 0.001%; ​​Peptone, 1.0%; Agar powder, 2.0%; H 2 O, 100 mL; pH 7.0.

[0036] Seed medium:

[0037] Peptone, 1.0%; Yeast powder, 0.5%; NaCl, 1.0%; Water, 100 mL; pH 7.0; Sterilize at 121°C for 20 minutes.

[0038] Fermentation medium:

[0039] Peptone, 1.0%; Yeast powder, 0.5%; NaCl, 1.0%; Water, 1L; pH 7.0; Sterilize at 121°C for 20 minutes.

[0040] Enrichment culture:

[0041] The preserved glycerol tube bacteria solution was connected to the seed culture medium, mixed evenly, and cultured at 37°C in a shaker at 180 rpm for 24 hours.

[0042] Plate screen bacteria:

[0043] Take the activated bacteria liquid for 100-fold moderate dilution, and spread it on a solid ...

Example Embodiment

[0063] Example 2

[0064] Process optimization of astaxanthin ester hydrolysis:

[0065] 1) The effect of reaction pH on the hydrolysis of astaxanthin esters

[0066] Weigh 2 mg of Haematococcus pluvialis oil, dissolve it with 0.5 mL of absolute ethanol, then weigh an appropriate amount of extracellular crude enzyme preparation, and dissolve the enzyme powder in buffer solutions of different pH values. The buffer solution used is as follows: citric acid buffer solution (100mM, pH 5.0-6.0), sodium phosphate buffer solution (100mM, pH 6.0-8.0), Tris-HCl buffer solution (100mM, pH 7.0-9.0) and glycine-sodium hydroxide buffer solution (100mM, pH9.0- 10.0), added to the reaction flask, filled with nitrogen, and placed in a 37°C water bath shaker to react for 30 minutes in the dark, that is, absolute ethanol: buffer solution = 1:10 (v / v), 5.5 mL of reaction system in total.

[0067] Such as Image 6 As shown, by adding different buffer solutions to the reaction system, the effect of differ...

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Abstract

The invention discloses a method for efficiently and rapidly preparing free astaxanthin. The strain for producing lipase and esterase is pseudomonas aeruginosa. Conditions such as the pH, the ratio ofa reaction solution, the temperature, the enzyme addition amount and time are determined. There is no need to add any acid or alkali in a reaction process of the technology, not only is the shortcoming that byproducts including astacin and semi-astacin are easily generated in the process of preparing the astaxanthin by means of a traditional saponification method overcome, but also the problems that the efficiency of preparing the astaxanthin by means of a single enzymolysis method is low and the cost is high are solved, and a novel approach is provided for environment-friendly scale production of the natural free astaxanthin.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for efficiently and rapidly preparing free astaxanthin by using an extracellular crude enzyme preparation of an astaxanthin esterase production strain. Background technique [0002] Natural astaxanthin (also known as 3,3′-dihydroxy-4,4′-diketone-β,β′-carotene) is an unsaturated terpene compound and a carotenoid, the main source In crustaceans such as Haematococcus pluvialis, Phaffia rhodozyme, shrimps, and crabs, it is purple-red and easily oxidized into astaxanthin (Astacene). Astaxanthin can quench singlet oxygen and scavenge free radicals. Its antioxidant capacity is 10 times that of other carotenoids and 500 times that of vitamin E. Astaxanthin has good physiological functions, the main functions include: anti-oxidation, anti-aging, protection of optic nerve, enhancement of human immune function, reduction of cerebral infarction, anti-inflammatory effect, pr...

Claims

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Application Information

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IPC IPC(8): C12P23/00
CPCC12P23/00
Inventor 毛相朝高新炜孙建安刘振薛长湖
Owner OCEAN UNIV OF CHINA
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