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Method for recycling glutamic acid fermentation waste thalli

A technology of glutamic acid and waste bacteria, which is applied in the field of fermentation engineering, can solve the problems of poor utilization of glutamic acid fermentation waste bacteria, and achieve the effect of saving raw material costs

Inactive Publication Date: 2018-10-26
LOTUS HEALTH GROUP COMPANY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of poor utilization of glutamic acid fermentation waste cells and provide a method for reusing glutamic acid fermentation waste cells

Method used

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  • Method for recycling glutamic acid fermentation waste thalli
  • Method for recycling glutamic acid fermentation waste thalli
  • Method for recycling glutamic acid fermentation waste thalli

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: the preparation of cell protein hydrolyzate

[0018] The method of ceramic membrane filtration is used to separate and obtain the waste cell bacterium slurry from the glutamic acid fermentation liquid, and the water content of the bacterium slurry is 55%. The pH value of the bacteria slurry was adjusted to 4.0 with 0.3mol / L sulfuric acid. Inject the bacteria slurry directly into the steam explosion equipment, set the steam pressure to 0.7Mpa, and maintain the pressure for 8 minutes. The shear force generated by the instantaneous deflation destroys the bacterial cells and completes the conversion of proteins into amino acids. The steam explosion is collected, and the supernatant and sediment are separated by high-speed centrifugation. The supernatant is the bacterial protein hydrolyzate.

Embodiment 2

[0019] Embodiment 2: Experimental comparison of glutamic acid fermentation

[0020] The bacterial protein hydrolyzate obtained in Example 1 was used instead of part of the organic nitrogen source for the glutamic acid fermentation process.

[0021] The fermentation medium composition of the experimental group is: 60g / L glucose, 5g / L dipotassium hydrogen phosphate, 30mg / L manganese sulfate, 30mg / L iron sulfate, 1.5g / L magnesium sulfate, 10mL / L corn steep liquor, 5mL / L Soybean meal hydrolyzate, 5mg / L vitamin mixture, 40mL / L cell protein hydrolyzate.

[0022] The composition of the fermentation medium of the control group was: 60g / L glucose, 5g / L dipotassium hydrogen phosphate, 30mg / L manganese sulfate, 30mg / L iron sulfate, 1.5g / L magnesium sulfate, 40mL / L corn steep liquor, 20mL / L Soybean meal hydrolyzate, 1g / L yeast powder, 5mg / L vitamin mixture.

[0023] The seed solution of the glutamic acid producing strain was inserted into the fermentation medium of the experimental grou...

Embodiment 3

[0026] Embodiment 3: the preparation of cell protein hydrolyzate

[0027] The method of ceramic membrane filtration is used to separate and obtain the waste cell bacteria slurry from the glutamic acid fermentation liquid, and the water content of the bacteria slurry is 60%. The pH value of the bacteria slurry was adjusted to 5.0 with 0.4mol / L hydrochloric acid. Inject the bacteria slurry directly into the steam explosion equipment, set the steam pressure to 0.8Mpa, and the pressure maintenance time to 10min, destroy the bacteria cells by the shear force generated by the instantaneous deflation, and complete the conversion of protein to amino acid. The steam explosion is collected, and the supernatant and sediment are separated by high-speed centrifugation. The supernatant is the bacterial protein hydrolyzate.

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Abstract

Disclosed is a method for recycling glutamic acid fermentation waste thalli. The method comprises the steps of separating waste thallus bacterial slurry from a glutamic acid fermentation broth; adjusting a pH value of the bacterial slurry; using a steam explosion method for treatment to obtain a thallus protein hydrolysate solution; using the thallus protein hydrolysate solution for preparing a glutamic acid fermentation culture medium; using the glutamic acid fermentation culture medium containing the thallus protein hydrolysate solution for glutamic acid fermentation. According to the method, the waste thalli do not need to be dried, or crushed or homogenized under high pressure, thallus protein does not need to be treated by high-concentration organic acid or expensive protease, the treatment cost of the waste thalli in glutamic acid fermentation production is significantly reduced accordingly, and secondary pollution caused in the treatment process is avoided. The glutamic acid fermentation culture medium containing the thallus protein hydrolysate solution can serve as an organic nitrogen source for promoting the growth of microorganisms and can be used for glutamic acid fermentation production. Accordingly, the raw material cost of the glutamic acid fermentation production is reduced. The method is especially suitable for popularization in production enterprises of glutamic acids and other amino acids.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a method for reusing glutamic acid fermentation waste cells. Background technique [0002] Glutamic acid and sodium glutamate (monosodium glutamate) are important bulk amino acid fermentation products in my country, with an annual output of 2.7 million tons, ranking first in the world. During the fermentation and extraction of glutamic acid and monosodium glutamate, a large amount of waste bacteria will be produced. According to statistics, every ton of product produced can produce 30-50 kg of waste cells, if discharged directly, it will cause serious environmental pollution and waste of resources. [0003] The waste cells of glutamic acid fermentation are rich in various nutrients such as protein, nucleic acid, sugar and vitamins. Therefore, in addition to being used as feed, glutamic acid cell proteins can also be used as raw materials to develop a...

Claims

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Application Information

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IPC IPC(8): C12P13/14
CPCC12P13/14
Inventor 范晓光韩洪军陈宁袁启发高立栋张顺棠井金峰
Owner LOTUS HEALTH GROUP COMPANY