Preparation method of high-strength hydrogel of surface layer embedding cell

Abstract

The invention provides a preparation method of high-strength hydrogel of a surface layer embedding cell. The preparation method comprises the steps of wrapping the surface of the cell with a layer ofcalcium phosphate shell at first so as to prevent the cell from being damaged in a polymerization process; dissolving sodium silicate, acrylic amide, a cross-linking agent and sodium alginate in waterto obtain a casting solution A, and dispersing the cell wrapped with the calcium phosphate shell in the casting solution A to obtain a casting solution B; scraping a mixed solution A into a film on aglass plate by using a film scraping stick, then pouring a mixed solution B on the film, and scrapping flatly by using the film scraping stick; and triggering acrylamide polymerization in an ultraviolet manner, hydrolyzing and releasing H+ from gluconic acid-delta-lactone by calcium ion aqueous solution cross-linking and soaking via a gluconic acid-delta-lactone aqueous solution, reacting H+ withcalcium metasilicate to generate calcium metasilicate of which the surface contains mesoporous silica gel, and meanwhile, reacting H+ with the calcium phosphate to release the protected cell. The mesoporous silica gel, calcium alginate and polyacrylamide are subjected to hydrogen-bond interaction, and thus, the strength of the hydrogel and the stability in a physiological environment are improved.

Description

technical field [0001] The invention relates to a preparation method of a high-strength hydrogel embedding cells on the surface, and belongs to the fields of biological materials and tissue engineering. Background technique [0002] The three elements of tissue engineering include seed cells, matrix materials and regulatory factors. In the past, tissue engineering scaffolds were usually designed from the perspective of materials science, rather than considering how to integrate implanted materials into tissue reconstruction from a biological perspective, often due to factors such as low cell utilization, fibrosis, rejection, and inflammatory reactions caused by scaffold degradation The expected repair effect cannot be achieved. Natural biomaterials have a wide range of sources, are non-toxic, and have good biocompatibility and biodegradability, but their processing and extraction are difficult and their mechanical properties are poor, which limits their application in tissu...

AI Technical Summary

Problems solved by technology

[0007] Aiming at the deficiencies of the prior art, the technical problem to be solved by the present invention is that it is difficult to control the cell density in two-dimensional culture, cell death is caused by deep cell embedding in three-dimensional culture, the gel strength is low under physiological environment, and the high-strength hydrogel is prepared The harsh environment in the process can damage cells and other problems

[0008] The present invention solves the problems that the two-dimensional culture is difficult to control the cell density, the three-dimensional culture causes cell death due to too deep cell embedding, the gel strength is low under the physiological environment, and the harsh environment of the high-strength hydrogel damages the cells during the preparation process. The technical solution is to provide a method for preparing a high-strength hydrogel with embedded cells on the surface