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Aquaculture antidote

A technology of aquaculture and antidote, applied in the direction of disinfectant, biocide, bactericide, etc., can solve the problems of lack of theoretical guidance, microbial demise, failure to reach, etc., achieve high safety performance, improve water quality, and good stability Effect

Active Publication Date: 2018-11-02
江苏远山生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although microecological preparations have achieved certain results in aquaculture, these strains are not inherent bacteria in the water environment, and may not survive in the water environment and the intestinal tract of farmed animals, let alone successfully reproduce and become the dominant flora for regulation. Added microbes could easily die out over time, study says
At the same time, even if indigenous bacteria are isolated from the aquaculture water environment or the intestinal tract of aquatic animals as beneficial bacteria, the expected effect will not be achieved due to the lack of sufficiently mature theoretical guidance.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of Bacillus coagulans seed solution

[0037] The Bacillus coagulans in this example was purchased from the Guangdong Microbial Culture Collection Center, and the strain number is GDMCC 1.645.

[0038] a. Strain cultivation: Transfer the preserved Bacillus coagulans to the slant of a test tube made of freshly prepared solid medium, and cultivate it at 37°C for 24 hours. Take the slant and transfer it to two 250ml eggplant bottle slants. Incubate at 37°C for 24 hours.

[0039] b. Seed tank cultivation: add liquid culture medium to the seed tank, the liquid content is 40%, adjust the pH to 7.6, stir, sterilize at 121°C for 30 minutes, turn on the cooling water to cool down, and control the pressure of the tank with sterile air. 0.05MPa, when the temperature drops to 36°C, wash the bacteria on the 2 eggplant bottles with 200ml sterile normal saline, collect the bacterial lawn lotion, inoculate it into the liquid medium in the seed tank, and keep it for 37 ℃, t...

Embodiment 2

[0043] Preparation of Enterococcus faecium seed solution

[0044] Enterococcus faecium in this example was purchased from the Guangdong Microbial Culture Collection Center, and the strain number is GDMCC 1.388.

[0045] a. Erlenmeyer flask seed culture: prepare liquid culture medium, adjust the pH value to 7, divide into 2000ml Erlenmeyer flasks, fill each bottle with 1500ml, sterilize at 121°C for 20 minutes, and inoculate the preserved Enterococcus faecium into the Erlenmeyer flasks at the same time after cooling In this method, cultured at 38°C for 20 hours under anaerobic conditions, transferred the grown Enterococcus faecium seeds into a 2000ml Erlenmeyer flask with 10% inoculum size, and cultured them statically at 37°C for 20 hours.

[0046] b. Seed tank cultivation: add liquid culture medium to the seed tank, the liquid content is 70%, adjust the pH value to 7, start stirring, sterilize at 121°C for 30 minutes, turn on cooling water to cool down, and pass sterile nitro...

Embodiment 3

[0049] Preparation of compound fermentation broth

[0050] a. Transfer the Enterococcus faecium seed liquid into a fermenter with 10% inoculum amount, 30-38°C, 0.04-0.06MPa, and culture statically until the Enterococcus faecium reaches the logarithmic growth phase;

[0051] B adjust the pH value in the fermenter at this time to be 7.2; and add the dipotassium hydrogen phosphate of calcium carbonate 5g / L and 1g / L;

[0052] c. The Bacillus coagulans seed solution was transferred to a fermenter with 10% inoculum amount, maintained at 37° C., 0.05 MPa, and 120 rpm, and carried out deep aeration and stirring culture for 26 hours.

[0053] When the spore rate of Bacillus coagulans in the fermentation tank liquid reaches 90%-95%, centrifuge to collect the concentrated bacterial liquid and fermentation supernatant; the concentrated bacterial liquid is used to prepare feed additives after drying; the fermentation supernatant is used Hydrochloric acid is used to adjust the pH value to ...

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Abstract

The invention discloses an aquaculture antidote which comprises, by weight, 5-15% of activated carbon, 5-15% of bioenzyme, 20-30% of diatomite, 10-20% of antibacterial peptide and the balance of compound fermentation liquid, wherein the compound fermentation liquid is supernate of mixing fermentation of bacillus coagulans and enterococcus faecium. The supernate obtained by in-depth fermentation ofbacillus coagulans and enterococcus faecium through co-culture is utilized and combined with the activated carbon, the bioenzyme, diatomite and the antibacterial peptide, so that the aquaculture antidote has effects of degrading organic matter in culture water, decompose and convert harmful substances, stabilizing pH value of the water and inhibiting pathogenic microorganisms.

Description

technical field [0001] The invention relates to the technical field of aquaculture preparations, in particular to an aquaculture antidote. Background technique [0002] With the emergence of a large number of large-scale aquaculture, the breeding density continues to increase, the excrement in the aquaculture water and the residue of the bait continue to increase, the pathogenic microorganisms multiply, the eutrophication of the aquaculture water is intensified, and the ecological environment is seriously damaged. , which has led to large-scale and frequent occurrence of various diseases and emergency situations in aquatic animals. At the same time, the long-term use and abuse of antibiotics and fungicides has further led to the residues of various drugs in the aquaculture water and the resistance and resistance of pathogenic microorganisms. The improvement of medicinal properties further destroys the ecological environment of the aquaculture water body. [0003] As we all ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34A01N59/00A01N37/46A01P1/00A01P3/00C02F103/20C02F101/30
CPCA01N37/46A01N59/00C02F1/50C02F1/52C02F3/34C02F2101/30C02F2103/20
Inventor 胡浩徐亚飞
Owner 江苏远山生物技术有限公司