Quantitative rapid deoxynivalenol detection card and detection method thereof

Abstract

The invention discloses a quantitative rapid deoxynivalenol detection card which comprises a sample pad, a nitrocellulose membrane and an absorbent pad arranged on a bottom plate in sequence, whereina detection line coated with a DON hapten-carrier protein coupling agent and a quality control line coated with a goat anti-rat antibody are arranged on the nitrocellulose membrane; a to-be-detected sample solution is mixed and incubated with latex microspheres coupled with a DON antibody before dropping to the sample pad. The detection method comprises the steps: preheating, adding liquid, incubating, reacting and reading the result. The detection card disclosed by the invention does not need a release pad containing a gold-labelled antibody and is used for detecting the latex microspheres coupled with the DON antibody mixed with the sample solution, the color is bright and easy to determine, and the coupling antibody is in a chemical bond coupling state, has excellent physical adsorptionstability with colloidal gold and is high in detection accuracy and precision degree; the batch-to-batch difference is small, and the cost is low; in addition, the detection method of the detection card disclosed by the invention is simple in operation and suitable for batch production and application.

Description

technical field [0001] The present invention relates to a quantitative rapid detection card and its detection method, in particular to a deoxynivalenol quantitative rapid detection card and its detection method, belonging to the technical field of biological detection, especially suitable for grain, oil and grain Quantitative and rapid detection of a class of deoxynivalenol. Background technique [0002] Vomitoxin (vomitoxin), also known as deoxynivalenol (deoxynivalenol, DON), chemical name 3α, 7α, 15-trihydroxy Fusarium-9-en-8-one, belongs to Trichothecenes mycophenolic compounds. It gets its name because it can cause pigs to vomit, and it also has certain harmful effects on the human body. The EU classification standard is a third-class carcinogen. Vomitoxin is highly toxic to both humans and animals, and can cause vomiting, diarrhea, skin irritation, food refusal, nervous disorders, miscarriage, stillbirth, etc. Pigs are the most sensitive animals to vomitoxin, followe...

AI Technical Summary

Problems solved by technology

[0005] (1) Colloidal gold itself is dark purple red, and the color is relatively light, so it is difficult to distinguish the positive and negative of the test results;

[0006] (2) The amount of gold produced in one batch of colloidal gold is at most about 10L, and the particle size deviation is difficult to accurately control. Quantitative products are mass-produced, and different batches are likely to cause large batch-to-batch differences, which will affect the accuracy and stability of the test results;

[0007] (3) Colloidal gold labeling technology is physical adsorption, which is less stable than chemical bond coupling;

[0008] (4) At present, colloidal gold test strips in the market are mainly qualitative products, which cannot meet people's application requirements for quantitative products that are fast, simple to operate, low in personnel requirements, low in cost, and visible to the naked eye. High false positive;

[0009] (5) The workload of burning gold and labeling antibodies is large, the process is cumbersome, the output is small, and waste of manpower and material resources

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