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Method for preparing tea saponin under coupled action of high-voltage pulsed electric field and biological enzymatic hydrolysis

A high-voltage pulsed electric field and tea saponin technology, which is applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., can solve the problem of unextracted tea saponin, achieve no chemical reagent pollution, and realize high-value utilization , the effect of improving the service life

Active Publication Date: 2018-11-09
华大国询(厦门)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no method for extracting tea saponin from tea seed dregs using high-voltage pulsed electric field technology.

Method used

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  • Method for preparing tea saponin under coupled action of high-voltage pulsed electric field and biological enzymatic hydrolysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1) Crush 500g of tea seed meal into powder, pass through a 60-mesh sieve to obtain tea seed meal powder; in a 90°C water bath, use petroleum ether with a boiling range of 60-90°C to remove the tea seed meal powder by Soxhlet extraction Grease, extract for 120 minutes, 5 minutes per siphon; after the Soxhlet extraction, dry naturally until there is no petroleum ether smell; take the degreased dry tea seed meal powder and add distilled water at a solid-to-liquid ratio of 1:20 (g:mL) , using a colloid mill (JMS-50 type, Langfang Guantong Machinery Co., Ltd., the same below) for homogenization for 5 minutes to obtain a uniform tea seed meal homogenate;

[0032] 2) The tea seed meal homogenate obtained in step 1) was pumped into the high-voltage pulsed electric field treatment chamber (SY-2-500 high-voltage pulsed electric field treatment device, South China University of Technology Light Industry and Food Science and Technology Co., Ltd. Researched and developed by the coll...

Embodiment 2

[0037] 1) Crush 500g of tea seed meal into powder, pass through a 60-mesh sieve to obtain tea seed meal powder; in a 90°C water bath, use petroleum ether with a boiling range of 60-90°C to remove the tea seed meal powder by Soxhlet extraction Grease, extract for 120 minutes, 5 minutes per siphon; after the Soxhlet extraction, dry naturally until there is no petroleum ether smell; take the degreased dry tea seed meal powder and add distilled water at a solid-to-liquid ratio of 1:10, and use colloidal grinding Slurry for 5 minutes to obtain a uniform tea seed meal homogenate;

[0038] 2) The tea seed meal homogenate obtained in step 1) is pumped into a high-voltage pulse electric field treatment chamber through a peristaltic pump at a flow rate of 5 mL / min, and subjected to high-voltage pulse electric field treatment with an electric field intensity of 20 kV / cm and a pulse number of 10;

[0039] 3) Take the tea seed dregs solution after the high-voltage pulse battery treatment, an...

Embodiment 3

[0043] 1) Crush 500g of tea seed meal into powder, pass through a 60-mesh sieve to obtain tea seed meal powder; in a 90°C water bath, use petroleum ether with a boiling range of 60-90°C to remove the tea seed meal powder by Soxhlet extraction Grease, extract for 120 minutes, 5 minutes per siphon; after the Soxhlet extraction, dry naturally until there is no petroleum ether smell; take the degreased dry tea seed meal powder and add distilled water at a solid-to-liquid ratio of 1:10, and use colloidal grinding Slurry for 5 minutes to obtain a uniform tea seed meal homogenate;

[0044] 2) The tea seed meal homogenate obtained in step 1) is pumped into a high-voltage pulse electric field treatment chamber through a peristaltic pump at a flow rate of 5 mL / min, and subjected to high-voltage pulse electric field treatment with an electric field intensity of 20 kV / cm and a pulse number of 10;

[0045] 3) Take the tea seed dregs solution after the high-voltage pulse battery treatment, ...

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Abstract

The invention discloses a method for preparing tea saponin under the coupled action of a high-voltage pulsed electric field and biological enzymatic hydrolysis. The method comprises the following steps: subjecting degreased tea seed meal powder to homogenization, then feeding the homogenized tea seed meal powder into a high-voltage pulsed electric field treatment chamber for treatment, and carrying out enzymatic hydrolysis so as to obtain a crude extract of tea saponin; and subjecting the crude extract of tea saponin to flocculation, then to microfiltration for removal of impurities and then to nanofiltration, concentration and purification so as to obtain the tea saponin. The method of the invention carries out deep processing and comprehensive utilization on tea seed meal, a by-product of processing in the tea seed oil industry in China, and the prepared tea saponin can be used as a natural non-ionic surfactant and is widely applied to daily chemicals, medicine, food, pesticides andother industries. The method of the invention is simple in operation process, low in cost and applicable to industrial production of various scales, and effectively solves the problem of discharge ofwaste produced in tea seed oil production and processing.

Description

technical field [0001] The invention belongs to the technical field of deep processing of agricultural and sideline products, and in particular relates to a method for preparing tea saponin through high-voltage pulsed electric field coupling biological enzymolysis. Background technique [0002] Camellia oleifera, also known as tea seed tree, tea oil tree, etc., is mostly distributed in the southern region. The output of camellia oleifera seeds in my country is 1 million tons per year. Camellia oleifera seeds can be pressed to obtain high-quality edible oil. The oleic acid content of camellia oleifera oil can be as high as 86%, and the linoleic acid content is 7-10%, which can be compared with olive oil. The output of camellia seed meal, which is the residue after oil extraction from camellia oleifera, is about 690,000 tons per year, and it shows an increasing trend every year. Tea seed meal is rich in nutrients, such as: about 20% tea saponin, 5-6% residual oil, 12-16% prot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07H15/256C07H1/08
CPCC07H1/08C07H15/256C07J63/008
Inventor 汤须崇颜志永黄志宏杨琼洪俊明邓爱华
Owner 华大国询(厦门)生物科技有限公司
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