Method for purifying tocotrienol by use of chromatographic column

Abstract

The invention provides a method for purifying tocotrienol by use of a chromatographic column. The method comprises steps as follows: (1) a tocotrienol crude product obtained after molecular distillation is selected, wherein the content of tocotrienol is 30%-70%; (2) packing: the chromatographic column is packed with a stationary phase adsorbent, and the stationary phase adsorbent is soaked in an initial solvent; (3) a raw material and the initial solvent are mixed, and the mixture is added to the upper end of the chromatographic column at the temperature of 30-50 DEG C at the speed of 5%-10% of the total quantity of packing materials each hour; (4) gradient elution is performed on the chromatographic column; (5) the eluted materials are collected section by section; (6) products collectedin each subsection are subjected to reduced pressure distillation, tocotrienol components are combined, and purified tocotrienol is obtained. The provided method is efficient, convenient and high in economic benefits, the purity of purified tocotrienol is high, completely no wastewater or waste residues are produced in the whole process, almost no waste gas is produced through matching of an efficient solvent recovery device, and almost no environmental protection facilities are required for treatment.

Description

technical field [0001] The invention belongs to the field of chemical engineering, and in particular relates to a method for purifying trienol by using a chromatographic column. Background technique [0002] Vitamin E (tocopherol) plays an important role in different growth stages of various animals, and is essential for the functional development of reproductive, muscle, nervous and immune systems. The most effective ingredient in vitamin E is d-alpha tocopherol, which is representative of its high activity and wide distribution, while trienol has stronger efficacy than d-alpha tocopherol, and is effective in anti-oxidation, anti-cancer, lowering cholesterol, etc. All aspects are stronger than d-alpha tocopherol. This is because trienols contain unsaturated side chains, which can more effectively penetrate into tissues containing saturated fatty acid layers, thus having very good antioxidant and free radical scavenging effects. In addition, it has good biocompatibility and...

AI Technical Summary

Problems solved by technology

The core process of this method is to use ion column or chromatographic column to purify the crude product of trienol, and after the trienol is freed, the used ion column and chromatographic column must be regenerated before they can be recycled, and a large amount of The mixed wastewater of waste alkaline water and waste oil is very difficult to follow-up environmental protection treatment, and the investment is large, which will still pollute the environment, which is neither economical nor environmentally friendly.