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Plant extract for promoting seed germination, and preparation method and application thereof

A plant extract and seed technology, applied in botany equipment and methods, seed and rhizome treatment, application, etc., can solve problems such as albinism, inconspicuousness, leggy crops, etc., and achieve the effect of improving efficiency

Inactive Publication Date: 2018-11-20
JILIN LIZE BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems that the current use of gibberellin to promote seed germination, too high concentration of gibberellin will cause excessive growth, albinism, deformity or withering of crops, while the effect is not obvious if the concentration is too low, the present invention provides a plant extract for promoting seed germination substances and their preparation methods and applications

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The preparation of embodiment 1 plant extract

[0045] Step 1, cultivating gibberellin-producing microbial strain Gibberella in LB medium;

[0046] Step 2, adjusting the pH of the fermentation medium to 6.5, and obtaining filter cake and filtrate containing microbial mycelium through filtration;

[0047] Step 3, washing the filter cake with water, combining the washing liquid and the filtrate to obtain an aqueous solution;

[0048] Step 4, the aqueous solution obtained in step 3 is concentrated by vacuum evaporation;

[0049] Step 5. Mix the solution obtained in Step 4 with methyl acetate at 5°C, and adjust the pH of the mixture to less than 2.0;

[0050] Step 6, separating the mixture obtained in step 5 into an aqueous phase and the first organic phase, and removing the first organic phase;

[0051] Step 7, using methyl acetate to extract the aqueous phase obtained in step 6 to obtain the second organic phase;

[0052] Step 8, combining the first organic phase and ...

Embodiment 2

[0057] The preparation of embodiment 2 plant extracts

[0058] Step 1, cultivating gibberellin-producing microbial strain Gibberella in LB medium;

[0059] Step 2, adjusting the pH of the fermentation medium to 6.8, and obtaining filter cake and filtrate containing microbial mycelium through filtration;

[0060] Step 3, washing the filter cake with water, combining the washing liquid and the filtrate to obtain an aqueous solution;

[0061] Step 4, the aqueous solution obtained in step 3 is concentrated by vacuum evaporation;

[0062] Step 5. Mix the solution obtained in Step 4 with ethyl acetate at 8°C, and adjust the pH of the mixture to less than 2.0;

[0063] Step 6, separating the mixture obtained in step 5 into an aqueous phase and the first organic phase, and removing the first organic phase;

[0064] Step 7, using ethyl acetate to extract the aqueous phase obtained in step 6 to obtain the second organic phase;

[0065] Step 8, combining the first organic phase and t...

Embodiment 3

[0070] The preparation of embodiment 3 plant extracts

[0071] Step 1, cultivating gibberellin-producing microbial strain Gibberella in LB medium;

[0072] Step 2, adjusting the pH of the fermentation medium to 7.0, and obtaining filter cake and filtrate containing microbial mycelium through filtration;

[0073] Step 3, washing the filter cake with water, combining the washing liquid and the filtrate to obtain an aqueous solution;

[0074] Step 4, the aqueous solution obtained in step 3 is concentrated by vacuum evaporation;

[0075] Step 5. Mix the solution obtained in Step 4 with an organic solvent (a mixture of propyl acetate, ethylene glycol monomethyl ether, and ethylene glycol monoethyl ether) at 10° C., and adjust the pH of the mixture to less than 2.0;

[0076] Step 6, separating the mixture obtained in step 5 into an aqueous phase and the first organic phase, and removing the first organic phase;

[0077] Step 7, using an organic solvent (propyl acetate, ethylene g...

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PUM

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Abstract

The invention discloses a plant extract for promoting seed germination, and a preparation method and the application thereof, and relates to the field of the seed cultivation. The preparation method comprises the following steps: cultivating gibberella, adjusting the pH value and filtering to obtain a filter cake and filtrate containing microorganism mycelium; washing the filter cake, combining the washing liquor and the filtrate; mixing with an organic solvent at 5-10 DEG C after evaporating and concentrating in vacuum; separating the mixture into an aqueous phase and a first organic phase, and removing the first organic phase; extracting the aqueous phase by adopting the organic solvent to obtain the second organic phase; combining the first organic phase and the second organic phase toform a concentrated organic solution; heating at 60-70 DEG C, stirring until the precipitation of the solid substance is terminated; cooling to a room temperature, filtering to obtain the precipitate;washing the precipitate by using the organic solvent, and drying to obtain a grey-white solid; and dissolving the grey-white solid to obtain the plant extract. The injury on the seed germination by excessive gibberella concentration is solved, the plant extract and the ordinary pesticide can be mixed and have a mutual synergy effect; the preparation method is simple, convenient, economical and labor-saving.

Description

technical field [0001] The invention relates to the technical field of seed cultivation, in particular to a plant extract for promoting seed germination, a preparation method and application thereof. Background technique [0002] Gibberellin is a plant growth regulator, mainly used to promote the growth and development of crops, early maturity, increase yield and break the dormancy of seeds, tubers, bulbs and other organs, promote germination, tillering, bolting, and increase fruit setting rate. However, gibberellic acid is insoluble in water, easily hydrolyzed, not resistant to high temperature, and has a short shelf life; the concentration of gibberellin is high, and if the concentration is too high, it will appear leggy, albino, until deformed or withered, and the effect is not obvious if the concentration is too low. [0003] Seed germination refers to a series of orderly physiological and morphogenetic processes of seeds starting from imbibition. Seed germination requi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/12A01P21/00A01C1/00
CPCA01C1/00A01N43/12
Inventor 朱博邹建国张中成崔朝贺张艳玲徐贺隋原邹丽辉
Owner JILIN LIZE BIOLOGICAL SCI & TECH CO LTD
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