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Bifidobacterium longum feed additive

A bifidobacterium longum and feed additive technology, which is applied in the field of bifidobacterium longum feed additives and bifidobacterium longum feed additives, can solve the problems of no direct use of nutritional value, short survival time, and strict requirements for nutritional components in farmed animals, and achieve Improving anti-immune ability, improving nutritional function, and increasing food preference

Inactive Publication Date: 2018-11-23
JIANGSU QIANYAOTANG GUOYI RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with lactic acid bacteria, bifidobacteria have the following disadvantages: (1) strict and complex requirements for nutrients, the medium must contain auxin or yeast extract to grow; (2) anaerobic conditions are more stringent; (3) at pH Short survival time in low environment
[0005] In order to keep the pH value in the stomach of the animals relatively constant, so that the fed food can continue to achieve the effect of acidification after entering the small intestine, acidulants such as citric acid, lactic acid, hydrochloric acid, and fumaric acid are often used in the product at the same time, but these acidulants Not only does it directly cause excessive acidity to the part of the microorganisms contained in the additive, which affects the survival of the bacteria, but at the same time, these acidified substances have no nutritional value for direct use of the farmed animals. At the same time, they are harmful to the gastrointestinal The props are very irritating, and its compatible application is not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Preparation of seabuckthorn freeze-dried powder, prickly pear freeze-dried powder, Physalis freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder:

[0053] Take fresh and mature seabuckthorn, prickly pear, physalis, black currant, cactus, remove impurities such as fruit stems and rotten fruits, rinse with 40°C aqueous solution containing 0.1% citric acid by weight, and rinse for 15 minutes under constant stirring. Rinse in pure water for 3 times, remove and dry the water; put seabuckthorn, prickly pear, physalis, blackcurrant, and cactus into plates and put them into the freezer, and quickly cool down to below -45°C; freeze the frozen seabuckthorn and prickly pear respectively , physalis, blackcurrant, and cactus are moved into the sublimation dryer, the sublimation temperature is controlled at -22--32°C, and the vacuum degree is 120-150Pa, so that the material is dried to moisture 100 mesh to obtain freeze-dried seabuckthorn powder, freeze-dr...

Embodiment 2

[0055] Use the corresponding optimized medium, use liquid culture fermentation equipment to separately culture Candida utilis, Candida tropicalis, brewer's yeast, brewer's yeast and feed yeast, and then use a high-speed centrifuge to separate the bacteria in the fermentation broth, Dry the sludge by vacuum freeze-drying to obtain Candida utilis dry powder with a live bacteria content of 20 billion / g, Candida tropicalis dry powder with a live bacteria content of 20 billion / g, and a live bacteria content of 200 Brewer's yeast dry powder with a live bacteria content of 20 billion / gram, brewer's yeast dry powder with a live bacteria content of 20 billion / gram, and feed yeast dry powder with a live bacteria content of 20 billion / gram.

Embodiment 3

[0057] Preparation of fermented eggshell powder:

[0058] Clean egg shells or one or more mixed egg shells of duck egg shells, goose egg shells, and quail egg shells, dry the water, put them in an oven, and dry them with hot air at 260°C for 2 hours, then take them out and let them cool. Grind into 45 mesh powder; weigh 80 kg of eggshell powder, add an appropriate amount of pure water to make the water content of the material 50% (W), then add 20 kg of dry feed yeast powder obtained in Example 2, mix well and put on a plate , placed in a fermentation room, and fermented at a temperature of 31-33°C for 60-72 hours. After the fermentation is over, the fermentation material is adjusted to a temperature of 82°C-92°C and a temperature of 80-90%. Ammonia reaction for 22-28 days, and then the material is dried in an electric oven at no more than 100°C until the moisture content is less than 5% (W), crushed with a universal grinder, and passed through 100 sieves to obtain fermented eg...

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PUM

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Abstract

The invention relates to a bifidobacterium longum feed additive. The bifidobacterium longum feed additive is characterized by being prepared by uniformly mixing, compositing and packaging bifidobacterium longum, hippophae rhamnoides freeze-dried powder, roxburgh rose freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, and cactus freeze-dried powder as well as the balance of fermented eggshell powder, wherein in every 1,000g of feed additive, 1,000 billion to 12,000 billion bifidobacterium longum, 5 to 50 g of hippophae rhamnoides freeze-dried powder, 5 to 50 g of roxburgh rose freeze-dried powder, 3 to 30 g of wintercherry freeze-dried powder, 3 to 30 g of blackcurrant freeze-dried powder, 50 to 150 g of cactus freeze-dried powder and the balance of fermented eggshell powder are added. The bifidobacterium longum feed additive is a single feed additive, is definite in viable count, and favorable for the user to have clear application and quantitativeselection. The freeze-dried powder of hippophae rhamnoides, roxburgh rose, wintercherry, blackcurrant, cactus and the fermented eggshell powder are used as a matrix of the additive, so that not onlycan the nutritional value of the bifidobacterium longum additive be increased, but also the metabolism of the animal can be promoted at the same time, the disease resistance of the body can be improved, the growth of the animal can be promoted, and finally the economic benefit of the breeding industry can be increased.

Description

technical field [0001] The invention belongs to the field of feed additives and relates to a bifidobacterium feed additive for breeding animals. Specifically relates to a bifidobacterium longum feed additive. Background technique [0002] Bifidobacterium was first isolated by Tissier in 1899 and is a Gram-positive bacillus. There are more than 30 known species of Bifidobacterium. Compared with lactic acid bacteria, bifidobacteria have the following disadvantages: (1) strict and complex requirements for nutrients, the medium must contain auxin or yeast extract to grow; (2) anaerobic conditions are more stringent; (3) at pH The survival time is short in low environment. Bifidobacteria can ferment sugars to produce a large amount of acetic acid and lactic acid, thereby inhibiting the growth and reproduction of potentially pathogenic Enterobacteriaceae and gradually achieving the balance of intestinal microorganisms. When the animal breeding environment, feed, disease, abuse...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K10/30A23K10/26A23K10/12
CPCA23K10/12A23K10/18A23K10/26A23K10/30A23V2400/533
Inventor 张奎昌张志年
Owner JIANGSU QIANYAOTANG GUOYI RES INST CO LTD
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